Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2926 and Psyr_2927 are separated by 110 nucleotides Psyr_2927 and Psyr_2928 are separated by 79 nucleotides Psyr_2928 and Psyr_2929 are separated by 58 nucleotides Psyr_2929 and Psyr_2930 are separated by 54 nucleotides
Psyr_2926: Psyr_2926 - General substrate transporter:Major facilitator superfamily, at 3,514,660 to 3,515,865
_2926
Psyr_2927: Psyr_2927 - transcriptional regulator, TetR family, at 3,515,976 to 3,516,602
_2927
Psyr_2928: Psyr_2928 - ABC transporter, transmembrane region:ABC transporter, at 3,516,682 to 3,518,535
_2928
Psyr_2929: Psyr_2929 - transcriptional regulator, LysR family, at 3,518,594 to 3,519,466
_2929
Psyr_2930: Psyr_2930 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 3,519,521 to 3,519,946
_2930
Position (kb)
3516
3517
3518
3519 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3515.694 kb on - strand, within Psyr_2926 at 3515.859 kb on + strand at 3515.860 kb on - strand at 3515.934 kb on - strand at 3516.016 kb on + strand at 3516.124 kb on - strand, within Psyr_2927 at 3516.186 kb on + strand, within Psyr_2927 at 3516.408 kb on - strand, within Psyr_2927 at 3516.500 kb on + strand, within Psyr_2927 at 3516.793 kb on + strand at 3516.794 kb on - strand at 3516.835 kb on + strand at 3516.925 kb on - strand, within Psyr_2928 at 3516.925 kb on - strand, within Psyr_2928 at 3517.004 kb on + strand, within Psyr_2928 at 3517.004 kb on + strand, within Psyr_2928 at 3517.187 kb on - strand, within Psyr_2928 at 3517.187 kb on - strand, within Psyr_2928 at 3517.187 kb on - strand, within Psyr_2928 at 3517.187 kb on - strand, within Psyr_2928 at 3517.282 kb on + strand, within Psyr_2928 at 3517.282 kb on + strand, within Psyr_2928 at 3517.282 kb on + strand, within Psyr_2928 at 3517.283 kb on - strand, within Psyr_2928 at 3517.283 kb on - strand, within Psyr_2928 at 3517.363 kb on + strand, within Psyr_2928 at 3517.363 kb on + strand, within Psyr_2928 at 3517.364 kb on - strand, within Psyr_2928 at 3517.364 kb on - strand, within Psyr_2928 at 3517.364 kb on - strand, within Psyr_2928 at 3517.382 kb on + strand, within Psyr_2928 at 3517.851 kb on - strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3517.912 kb on + strand, within Psyr_2928 at 3518.050 kb on + strand, within Psyr_2928 at 3518.461 kb on + strand at 3518.461 kb on + strand at 3518.461 kb on + strand at 3518.461 kb on + strand at 3518.462 kb on - strand at 3518.582 kb on + strand at 3518.618 kb on - strand at 3518.830 kb on + strand, within Psyr_2929 at 3518.831 kb on - strand, within Psyr_2929 at 3518.886 kb on + strand, within Psyr_2929 at 3518.886 kb on + strand, within Psyr_2929 at 3519.091 kb on + strand, within Psyr_2929 at 3519.361 kb on + strand, within Psyr_2929 at 3519.361 kb on + strand, within Psyr_2929 at 3519.362 kb on - strand, within Psyr_2929 at 3519.362 kb on - strand, within Psyr_2929 at 3519.362 kb on - strand, within Psyr_2929 at 3519.387 kb on + strand at 3519.387 kb on + strand at 3519.388 kb on - strand at 3519.388 kb on - strand at 3519.388 kb on - strand at 3519.388 kb on - strand at 3519.519 kb on + strand at 3519.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 3,515,694 - Psyr_2926 0.86 +2.3 3,515,859 + -0.1 3,515,860 - +0.3 3,515,934 - -1.5 3,516,016 + +2.3 3,516,124 - Psyr_2927 0.24 -1.0 3,516,186 + Psyr_2927 0.33 +0.1 3,516,408 - Psyr_2927 0.69 -2.0 3,516,500 + Psyr_2927 0.84 +0.7 3,516,793 + +1.9 3,516,794 - +1.6 3,516,835 + +0.1 3,516,925 - Psyr_2928 0.13 -0.6 3,516,925 - Psyr_2928 0.13 -0.4 3,517,004 + Psyr_2928 0.17 -1.0 3,517,004 + Psyr_2928 0.17 -2.2 3,517,187 - Psyr_2928 0.27 -1.2 3,517,187 - Psyr_2928 0.27 -0.7 3,517,187 - Psyr_2928 0.27 -0.3 3,517,187 - Psyr_2928 0.27 -1.0 3,517,282 + Psyr_2928 0.32 +1.5 3,517,282 + Psyr_2928 0.32 +0.3 3,517,282 + Psyr_2928 0.32 +2.0 3,517,283 - Psyr_2928 0.32 +1.8 3,517,283 - Psyr_2928 0.32 -2.0 3,517,363 + Psyr_2928 0.37 -1.9 3,517,363 + Psyr_2928 0.37 +1.3 3,517,364 - Psyr_2928 0.37 +1.6 3,517,364 - Psyr_2928 0.37 -0.7 3,517,364 - Psyr_2928 0.37 +0.4 3,517,382 + Psyr_2928 0.38 +0.3 3,517,851 - Psyr_2928 0.63 +1.8 3,517,912 + Psyr_2928 0.66 +1.5 3,517,912 + Psyr_2928 0.66 -1.9 3,517,912 + Psyr_2928 0.66 +0.7 3,517,912 + Psyr_2928 0.66 +1.4 3,517,912 + Psyr_2928 0.66 -0.3 3,517,912 + Psyr_2928 0.66 +1.0 3,517,912 + Psyr_2928 0.66 -1.4 3,517,912 + Psyr_2928 0.66 -0.9 3,518,050 + Psyr_2928 0.74 +0.4 3,518,461 + -1.4 3,518,461 + -1.5 3,518,461 + +0.5 3,518,461 + +2.2 3,518,462 - -1.1 3,518,582 + -0.9 3,518,618 - +0.2 3,518,830 + Psyr_2929 0.27 -0.0 3,518,831 - Psyr_2929 0.27 -0.9 3,518,886 + Psyr_2929 0.33 -2.5 3,518,886 + Psyr_2929 0.33 +0.3 3,519,091 + Psyr_2929 0.57 -0.0 3,519,361 + Psyr_2929 0.88 +0.1 3,519,361 + Psyr_2929 0.88 +1.6 3,519,362 - Psyr_2929 0.88 +3.2 3,519,362 - Psyr_2929 0.88 -2.7 3,519,362 - Psyr_2929 0.88 +2.5 3,519,387 + -1.3 3,519,387 + -0.0 3,519,388 - +0.3 3,519,388 - +1.0 3,519,388 - +1.3 3,519,388 - +0.1 3,519,519 + +2.3 3,519,520 - -0.7
Or see this region's nucleotide sequence