Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2854 and Psyr_2855 are separated by 23 nucleotides Psyr_2855 and Psyr_2856 are separated by 158 nucleotides
Psyr_2854: Psyr_2854 - conserved hypothetical protein, at 3,425,751 to 3,426,734
_2854
Psyr_2855: Psyr_2855 - methionine synthase (B12-independent), at 3,426,758 to 3,427,789
_2855
Psyr_2856: Psyr_2856 - Chitin-binding, domain 3, at 3,427,948 to 3,428,691
_2856
Position (kb)
3426
3427
3428 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3425.802 kb on + strand at 3425.883 kb on - strand, within Psyr_2854 at 3425.885 kb on + strand, within Psyr_2854 at 3425.954 kb on + strand, within Psyr_2854 at 3425.996 kb on + strand, within Psyr_2854 at 3426.109 kb on - strand, within Psyr_2854 at 3426.238 kb on - strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.298 kb on - strand, within Psyr_2854 at 3426.328 kb on + strand, within Psyr_2854 at 3426.373 kb on - strand, within Psyr_2854 at 3426.403 kb on - strand, within Psyr_2854 at 3426.468 kb on + strand, within Psyr_2854 at 3426.469 kb on - strand, within Psyr_2854 at 3426.486 kb on - strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.618 kb on + strand, within Psyr_2854 at 3426.619 kb on - strand, within Psyr_2854 at 3426.690 kb on + strand at 3426.690 kb on + strand at 3426.740 kb on + strand at 3426.751 kb on + strand at 3426.751 kb on + strand at 3426.757 kb on + strand at 3426.970 kb on + strand, within Psyr_2855 at 3426.970 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3427.021 kb on + strand, within Psyr_2855 at 3427.194 kb on - strand, within Psyr_2855 at 3427.369 kb on + strand at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.461 kb on - strand, within Psyr_2855 at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.800 kb on - strand at 3427.800 kb on - strand at 3427.800 kb on - strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.877 kb on - strand at 3427.941 kb on + strand at 3428.079 kb on + strand, within Psyr_2856 at 3428.080 kb on - strand, within Psyr_2856 at 3428.080 kb on - strand, within Psyr_2856 at 3428.192 kb on + strand, within Psyr_2856 at 3428.192 kb on + strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand at 3428.193 kb on - strand, within Psyr_2856 at 3428.256 kb on + strand, within Psyr_2856 at 3428.257 kb on - strand, within Psyr_2856 at 3428.284 kb on + strand, within Psyr_2856 at 3428.284 kb on + strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.294 kb on + strand, within Psyr_2856 at 3428.304 kb on + strand, within Psyr_2856 at 3428.304 kb on + strand, within Psyr_2856 at 3428.305 kb on - strand, within Psyr_2856 at 3428.377 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.447 kb on - strand at 3428.447 kb on - strand, within Psyr_2856 at 3428.447 kb on - strand, within Psyr_2856 at 3428.505 kb on + strand, within Psyr_2856 at 3428.508 kb on + strand, within Psyr_2856 at 3428.509 kb on - strand, within Psyr_2856 at 3428.509 kb on - strand, within Psyr_2856 at 3428.624 kb on + strand at 3428.625 kb on - strand at 3428.625 kb on - strand at 3428.625 kb on - strand at 3428.650 kb on + strand at 3428.697 kb on + strand at 3428.698 kb on - strand at 3428.707 kb on + strand at 3428.708 kb on - strand at 3428.767 kb on - strand at 3428.770 kb on + strand at 3428.771 kb on - strand at 3428.771 kb on - strand at 3428.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 3,425,802 + -1.6 3,425,883 - Psyr_2854 0.13 -3.4 3,425,885 + Psyr_2854 0.14 -1.0 3,425,954 + Psyr_2854 0.21 -0.8 3,425,996 + Psyr_2854 0.25 -1.3 3,426,109 - Psyr_2854 0.36 +1.1 3,426,238 - Psyr_2854 0.49 +2.1 3,426,297 + Psyr_2854 0.55 -1.1 3,426,297 + Psyr_2854 0.55 -0.7 3,426,298 - Psyr_2854 0.56 -0.1 3,426,328 + Psyr_2854 0.59 +1.5 3,426,373 - Psyr_2854 0.63 -2.4 3,426,403 - Psyr_2854 0.66 -0.1 3,426,468 + Psyr_2854 0.73 -1.7 3,426,469 - Psyr_2854 0.73 -0.3 3,426,486 - Psyr_2854 0.75 +0.1 3,426,540 + Psyr_2854 0.80 +0.5 3,426,540 + Psyr_2854 0.80 +2.1 3,426,540 + Psyr_2854 0.80 +1.3 3,426,541 - Psyr_2854 0.80 -1.8 3,426,541 - Psyr_2854 0.80 +0.3 3,426,541 - Psyr_2854 0.80 +0.3 3,426,541 - Psyr_2854 0.80 -1.1 3,426,618 + Psyr_2854 0.88 -1.0 3,426,619 - Psyr_2854 0.88 -0.6 3,426,690 + +0.8 3,426,690 + +3.0 3,426,740 + +1.7 3,426,751 + -1.6 3,426,751 + +2.1 3,426,757 + +0.6 3,426,970 + Psyr_2855 0.21 -2.0 3,426,970 + Psyr_2855 0.21 +0.3 3,426,976 + Psyr_2855 0.21 -3.5 3,426,976 + Psyr_2855 0.21 -2.0 3,427,021 + Psyr_2855 0.25 -0.7 3,427,194 - Psyr_2855 0.42 +0.2 3,427,369 + +1.3 3,427,390 + Psyr_2855 0.61 -1.5 3,427,390 + Psyr_2855 0.61 -0.8 3,427,390 + Psyr_2855 0.61 -1.5 3,427,391 - Psyr_2855 0.61 -2.1 3,427,391 - Psyr_2855 0.61 +1.0 3,427,461 - Psyr_2855 0.68 -1.3 3,427,799 + -1.0 3,427,799 + -0.5 3,427,799 + -2.1 3,427,799 + -0.8 3,427,800 - -3.8 3,427,800 - -1.2 3,427,800 - +0.7 3,427,876 + -0.7 3,427,876 + -0.4 3,427,876 + -1.1 3,427,876 + -1.0 3,427,876 + -0.6 3,427,876 + -0.2 3,427,876 + -0.7 3,427,877 - +1.0 3,427,941 + +0.2 3,428,079 + Psyr_2856 0.18 +1.3 3,428,080 - Psyr_2856 0.18 -0.8 3,428,080 - Psyr_2856 0.18 +0.9 3,428,192 + Psyr_2856 0.33 +1.2 3,428,192 + Psyr_2856 0.33 -2.6 3,428,193 - Psyr_2856 0.33 +1.2 3,428,193 - Psyr_2856 0.33 +0.2 3,428,193 - Psyr_2856 0.33 +0.9 3,428,193 - Psyr_2856 0.33 -2.4 3,428,193 - -2.4 3,428,193 - Psyr_2856 0.33 -1.3 3,428,256 + Psyr_2856 0.41 +1.6 3,428,257 - Psyr_2856 0.42 -0.7 3,428,284 + Psyr_2856 0.45 -0.0 3,428,284 + Psyr_2856 0.45 +0.4 3,428,285 - Psyr_2856 0.45 -3.1 3,428,285 - Psyr_2856 0.45 +0.4 3,428,285 - Psyr_2856 0.45 -0.7 3,428,294 + Psyr_2856 0.47 -2.3 3,428,304 + Psyr_2856 0.48 +0.3 3,428,304 + Psyr_2856 0.48 +0.2 3,428,305 - Psyr_2856 0.48 +1.9 3,428,377 + Psyr_2856 0.58 -3.3 3,428,446 + Psyr_2856 0.67 +0.7 3,428,446 + Psyr_2856 0.67 +2.1 3,428,446 + Psyr_2856 0.67 +0.4 3,428,446 + Psyr_2856 0.67 +0.7 3,428,446 + Psyr_2856 0.67 +1.5 3,428,447 - -0.5 3,428,447 - Psyr_2856 0.67 -1.5 3,428,447 - Psyr_2856 0.67 -1.7 3,428,505 + Psyr_2856 0.75 -0.9 3,428,508 + Psyr_2856 0.75 -2.3 3,428,509 - Psyr_2856 0.75 -0.3 3,428,509 - Psyr_2856 0.75 -1.1 3,428,624 + -0.1 3,428,625 - -1.8 3,428,625 - +0.4 3,428,625 - +1.4 3,428,650 + +1.3 3,428,697 + +0.4 3,428,698 - +0.2 3,428,707 + +1.3 3,428,708 - -1.2 3,428,767 - +0.8 3,428,770 + -1.7 3,428,771 - -0.3 3,428,771 - -1.0 3,428,771 - -0.4
Or see this region's nucleotide sequence