Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2749 and Psyr_2750 overlap by 8 nucleotides Psyr_2750 and Psyr_2751 are separated by 333 nucleotides
Psyr_2749: Psyr_2749 - Methionine--tRNA ligase, at 3,337,287 to 3,338,786
_2749
Psyr_2750: Psyr_2750 - conserved hypothetical protein, at 3,338,779 to 3,339,171
_2750
Psyr_2751: Psyr_2751 - Phage integrase:Phage integrase, N-terminal SAM-like protein, at 3,339,505 to 3,340,470
_2751
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3337.999 kb on + strand, within Psyr_2749 at 3337.999 kb on + strand, within Psyr_2749 at 3338.021 kb on - strand, within Psyr_2749 at 3338.021 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.645 kb on - strand at 3338.730 kb on + strand at 3338.730 kb on + strand at 3338.730 kb on + strand at 3338.731 kb on - strand at 3338.731 kb on - strand at 3338.731 kb on - strand at 3338.780 kb on + strand at 3338.781 kb on - strand at 3338.781 kb on - strand at 3338.781 kb on - strand at 3338.834 kb on + strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3339.124 kb on + strand, within Psyr_2750 at 3339.148 kb on - strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.261 kb on - strand at 3339.261 kb on - strand at 3339.304 kb on - strand at 3339.304 kb on - strand at 3339.304 kb on - strand at 3339.341 kb on - strand at 3339.365 kb on - strand at 3339.460 kb on - strand at 3339.469 kb on - strand at 3339.713 kb on + strand, within Psyr_2751 at 3339.713 kb on + strand, within Psyr_2751 at 3339.713 kb on + strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.747 kb on + strand, within Psyr_2751 at 3339.866 kb on + strand, within Psyr_2751 at 3339.977 kb on + strand, within Psyr_2751
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 3,337,999 + Psyr_2749 0.47 +3.3 3,337,999 + Psyr_2749 0.47 -2.1 3,338,021 - Psyr_2749 0.49 -1.4 3,338,021 - Psyr_2749 0.49 -0.3 3,338,351 - Psyr_2749 0.71 +3.9 3,338,351 - Psyr_2749 0.71 +2.9 3,338,351 - Psyr_2749 0.71 +0.5 3,338,526 + Psyr_2749 0.83 -2.4 3,338,526 + Psyr_2749 0.83 -3.0 3,338,526 + Psyr_2749 0.83 -0.2 3,338,526 + Psyr_2749 0.83 -1.8 3,338,526 + Psyr_2749 0.83 -0.1 3,338,527 - Psyr_2749 0.83 -2.6 3,338,527 - Psyr_2749 0.83 -1.8 3,338,527 - Psyr_2749 0.83 +0.1 3,338,527 - Psyr_2749 0.83 +0.1 3,338,623 + Psyr_2749 0.89 -0.2 3,338,623 + Psyr_2749 0.89 -0.1 3,338,623 + Psyr_2749 0.89 +0.8 3,338,623 + Psyr_2749 0.89 -0.1 3,338,623 + Psyr_2749 0.89 -1.0 3,338,624 - Psyr_2749 0.89 -0.2 3,338,624 - Psyr_2749 0.89 +0.9 3,338,624 - Psyr_2749 0.89 -1.5 3,338,624 - Psyr_2749 0.89 -0.2 3,338,624 - Psyr_2749 0.89 -1.8 3,338,624 - Psyr_2749 0.89 -1.1 3,338,624 - Psyr_2749 0.89 -0.6 3,338,624 - Psyr_2749 0.89 +0.1 3,338,645 - -1.1 3,338,730 + -1.5 3,338,730 + -1.3 3,338,730 + -2.4 3,338,731 - +0.3 3,338,731 - +1.1 3,338,731 - -4.0 3,338,780 + +0.3 3,338,781 - -0.3 3,338,781 - -0.4 3,338,781 - +1.3 3,338,834 + Psyr_2750 0.14 +0.4 3,338,835 - Psyr_2750 0.14 +0.5 3,338,835 - Psyr_2750 0.14 -1.2 3,338,835 - Psyr_2750 0.14 +2.2 3,338,835 - Psyr_2750 0.14 +1.9 3,338,835 - Psyr_2750 0.14 +0.8 3,338,835 - Psyr_2750 0.14 -0.2 3,338,835 - Psyr_2750 0.14 +0.6 3,338,858 + Psyr_2750 0.20 +1.3 3,338,858 + Psyr_2750 0.20 -1.3 3,338,858 + Psyr_2750 0.20 +1.3 3,338,858 + Psyr_2750 0.20 -1.8 3,338,858 + Psyr_2750 0.20 -0.2 3,338,858 + Psyr_2750 0.20 +1.5 3,338,858 + Psyr_2750 0.20 -0.4 3,338,859 - Psyr_2750 0.20 -1.2 3,338,859 - Psyr_2750 0.20 +0.8 3,338,859 - Psyr_2750 0.20 -1.7 3,338,859 - Psyr_2750 0.20 -0.8 3,338,894 + Psyr_2750 0.29 -0.4 3,338,894 + Psyr_2750 0.29 -1.1 3,338,894 + Psyr_2750 0.29 +0.1 3,338,894 + Psyr_2750 0.29 -1.7 3,338,895 - Psyr_2750 0.30 +0.0 3,338,895 - Psyr_2750 0.30 -1.6 3,338,895 - Psyr_2750 0.30 -1.5 3,338,895 - Psyr_2750 0.30 +0.5 3,339,124 + Psyr_2750 0.88 +0.6 3,339,148 - +0.3 3,339,154 + +1.7 3,339,154 + -0.6 3,339,154 + -2.4 3,339,154 + +0.4 3,339,154 + -1.1 3,339,154 + +0.3 3,339,154 + -2.7 3,339,154 + -1.3 3,339,154 + +0.6 3,339,155 - +3.3 3,339,155 - -0.1 3,339,155 - -0.4 3,339,155 - +0.1 3,339,155 - +2.8 3,339,155 - -1.2 3,339,155 - -0.6 3,339,155 - -1.5 3,339,155 - -1.2 3,339,261 - +1.2 3,339,261 - +0.0 3,339,304 - +1.4 3,339,304 - -0.4 3,339,304 - -0.7 3,339,341 - -1.7 3,339,365 - -0.4 3,339,460 - -3.5 3,339,469 - -0.6 3,339,713 + Psyr_2751 0.22 -0.4 3,339,713 + Psyr_2751 0.22 -1.2 3,339,713 + Psyr_2751 0.22 +0.6 3,339,714 - Psyr_2751 0.22 -0.1 3,339,714 - Psyr_2751 0.22 +0.0 3,339,714 - Psyr_2751 0.22 -2.1 3,339,714 - Psyr_2751 0.22 +0.0 3,339,747 + Psyr_2751 0.25 +0.8 3,339,866 + Psyr_2751 0.37 +0.0 3,339,977 + Psyr_2751 0.49 -0.4
Or see this region's nucleotide sequence