Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2022

Experiment: KB with Plumbagin 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2021 and Psyr_2022 are separated by 284 nucleotidesPsyr_2022 and Psyr_2023 are separated by 24 nucleotidesPsyr_2023 and Psyr_2024 overlap by 4 nucleotidesPsyr_2024 and Psyr_2025 are separated by 62 nucleotides Psyr_2021: Psyr_2021 - Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,345,809 to 2,347,746 _2021 Psyr_2022: Psyr_2022 - Glycerol-3-phosphate dehydrogenase (NAD(P)+), at 2,348,031 to 2,349,056 _2022 Psyr_2023: Psyr_2023 - conserved hypothetical protein, at 2,349,081 to 2,349,425 _2023 Psyr_2024: Psyr_2024 - Phosphohistidine phosphatase SixA, at 2,349,422 to 2,349,877 _2024 Psyr_2025: Psyr_2025 - Phenylacetic acid degradation-related protein, at 2,349,940 to 2,350,383 _2025 Position (kb) 2348 2349 2350Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2347.109 kb on + strand, within Psyr_2021at 2347.110 kb on - strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.468 kb on - strand, within Psyr_2021at 2347.493 kb on + strand, within Psyr_2021at 2347.493 kb on + strand, within Psyr_2021at 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.653 kb on + strandat 2347.654 kb on + strandat 2347.722 kb on + strandat 2347.722 kb on + strandat 2347.985 kb on + strandat 2347.986 kb on - strandat 2347.988 kb on - strandat 2348.030 kb on + strandat 2348.926 kb on - strand, within Psyr_2022at 2349.050 kb on + strandat 2349.050 kb on + strandat 2349.207 kb on + strand, within Psyr_2023at 2349.270 kb on + strand, within Psyr_2023at 2349.653 kb on - strand, within Psyr_2024at 2349.714 kb on + strand, within Psyr_2024at 2349.948 kb on - strandat 2349.948 kb on - strandat 2349.948 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM
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2,347,109 + Psyr_2021 0.67 +2.1
2,347,110 - Psyr_2021 0.67 +0.3
2,347,244 - Psyr_2021 0.74 +1.7
2,347,244 - Psyr_2021 0.74 -0.2
2,347,304 + Psyr_2021 0.77 -2.0
2,347,304 + Psyr_2021 0.77 +0.1
2,347,304 + Psyr_2021 0.77 +0.9
2,347,450 + Psyr_2021 0.85 +1.7
2,347,450 + Psyr_2021 0.85 +1.8
2,347,450 + Psyr_2021 0.85 -0.2
2,347,451 - Psyr_2021 0.85 +1.7
2,347,451 - Psyr_2021 0.85 -0.5
2,347,451 - Psyr_2021 0.85 -0.6
2,347,451 - Psyr_2021 0.85 -2.1
2,347,451 - Psyr_2021 0.85 +0.8
2,347,454 + Psyr_2021 0.85 -1.5
2,347,454 + Psyr_2021 0.85 -1.7
2,347,454 + Psyr_2021 0.85 +0.2
2,347,454 + Psyr_2021 0.85 +1.8
2,347,454 + Psyr_2021 0.85 -2.7
2,347,454 + Psyr_2021 0.85 -2.7
2,347,454 + Psyr_2021 0.85 +1.7
2,347,454 + Psyr_2021 0.85 +0.6
2,347,454 + Psyr_2021 0.85 +0.1
2,347,455 - Psyr_2021 0.85 +1.4
2,347,455 - Psyr_2021 0.85 +1.1
2,347,455 - Psyr_2021 0.85 -3.3
2,347,455 - Psyr_2021 0.85 +1.8
2,347,455 - Psyr_2021 0.85 +1.3
2,347,455 - Psyr_2021 0.85 +1.7
2,347,455 - Psyr_2021 0.85 +1.0
2,347,455 - Psyr_2021 0.85 +1.6
2,347,468 - Psyr_2021 0.86 +0.8
2,347,493 + Psyr_2021 0.87 +0.9
2,347,493 + Psyr_2021 0.87 +0.0
2,347,641 + +1.4
2,347,641 + -0.6
2,347,641 + -0.4
2,347,641 + -1.0
2,347,645 + +0.2
2,347,645 + +1.4
2,347,645 + -0.5
2,347,645 + +1.1
2,347,645 + +1.4
2,347,645 + -0.2
2,347,646 - +0.8
2,347,646 - -1.0
2,347,646 - +0.3
2,347,646 - -1.9
2,347,646 - +0.8
2,347,653 + -0.4
2,347,654 + +0.7
2,347,722 + -0.9
2,347,722 + +0.2
2,347,985 + +2.9
2,347,986 - -0.7
2,347,988 - -0.9
2,348,030 + -2.0
2,348,926 - Psyr_2022 0.87 +0.1
2,349,050 + +0.1
2,349,050 + -1.4
2,349,207 + Psyr_2023 0.37 +1.1
2,349,270 + Psyr_2023 0.55 +0.1
2,349,653 - Psyr_2024 0.51 +1.4
2,349,714 + Psyr_2024 0.64 +0.7
2,349,948 - +0.5
2,349,948 - -1.6
2,349,948 - -2.8

Or see this region's nucleotide sequence