Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1890 and Psyr_1891 are separated by 218 nucleotides Psyr_1891 and Psyr_RNA35 are separated by 205 nucleotides Psyr_RNA35 and Psyr_1892 are separated by 178 nucleotides Psyr_1892 and Psyr_1893 are separated by 113 nucleotides Psyr_1893 and Psyr_1894 are separated by 13 nucleotides
Psyr_1890: Psyr_1890 - type III effector HopAP1, at 2,160,584 to 2,161,180
_1890
Psyr_1891: Psyr_1891 - hypothetical protein, at 2,161,399 to 2,161,701
_1891
Psyr_RNA35: Psyr_RNA35 - tRNA-Ser, at 2,161,907 to 2,161,996
_RNA35
Psyr_1892: Psyr_1892 - Short-chain dehydrogenase/reductase SDR, at 2,162,175 to 2,162,924
_1892
Psyr_1893: Psyr_1893 - Isochorismatase hydrolase, at 2,163,038 to 2,163,604
_1893
Psyr_1894: Psyr_1894 - SNF2-related:Helicase, C-terminal:SWIM Zn-finger, at 2,163,618 to 2,166,362
_1894
Position (kb)
2162
2163 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2161.229 kb on + strand at 2161.229 kb on + strand at 2161.229 kb on + strand at 2161.229 kb on + strand at 2161.229 kb on + strand at 2161.229 kb on + strand at 2161.230 kb on - strand at 2161.230 kb on - strand at 2161.230 kb on - strand at 2161.230 kb on - strand at 2161.230 kb on - strand at 2161.230 kb on - strand at 2161.240 kb on - strand at 2161.290 kb on + strand at 2161.290 kb on + strand at 2161.291 kb on - strand at 2161.291 kb on - strand at 2161.291 kb on - strand at 2161.316 kb on + strand at 2161.317 kb on - strand at 2161.317 kb on - strand at 2161.317 kb on - strand at 2161.317 kb on - strand at 2161.350 kb on + strand at 2161.350 kb on + strand at 2161.350 kb on + strand at 2161.351 kb on - strand at 2161.351 kb on - strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.361 kb on + strand at 2161.362 kb on - strand at 2161.362 kb on - strand at 2161.362 kb on - strand at 2161.362 kb on - strand at 2161.362 kb on - strand at 2161.366 kb on - strand at 2161.366 kb on - strand at 2161.366 kb on - strand at 2161.512 kb on + strand, within Psyr_1891 at 2161.512 kb on + strand, within Psyr_1891 at 2161.512 kb on + strand, within Psyr_1891 at 2161.512 kb on + strand, within Psyr_1891 at 2161.513 kb on - strand, within Psyr_1891 at 2161.602 kb on + strand, within Psyr_1891 at 2161.606 kb on - strand, within Psyr_1891 at 2161.606 kb on - strand, within Psyr_1891 at 2161.612 kb on + strand, within Psyr_1891 at 2161.612 kb on + strand, within Psyr_1891 at 2161.613 kb on - strand, within Psyr_1891 at 2161.613 kb on - strand, within Psyr_1891 at 2161.655 kb on - strand, within Psyr_1891 at 2161.760 kb on + strand at 2161.760 kb on + strand at 2161.760 kb on + strand at 2161.760 kb on + strand at 2161.760 kb on + strand at 2161.761 kb on - strand at 2161.761 kb on - strand at 2161.761 kb on - strand at 2161.805 kb on + strand at 2161.899 kb on + strand at 2162.221 kb on + strand at 2162.222 kb on - strand at 2162.222 kb on - strand at 2162.222 kb on - strand at 2162.528 kb on + strand, within Psyr_1892 at 2162.528 kb on + strand, within Psyr_1892 at 2162.528 kb on + strand, within Psyr_1892 at 2162.528 kb on + strand, within Psyr_1892 at 2162.528 kb on + strand, within Psyr_1892 at 2162.528 kb on + strand, within Psyr_1892 at 2162.529 kb on - strand, within Psyr_1892 at 2162.529 kb on - strand, within Psyr_1892 at 2162.529 kb on - strand, within Psyr_1892 at 2162.529 kb on - strand, within Psyr_1892 at 2162.806 kb on + strand, within Psyr_1892 at 2162.806 kb on + strand, within Psyr_1892 at 2162.806 kb on + strand, within Psyr_1892 at 2162.806 kb on + strand, within Psyr_1892 at 2162.806 kb on + strand, within Psyr_1892 at 2162.810 kb on + strand, within Psyr_1892 at 2162.810 kb on + strand, within Psyr_1892 at 2162.892 kb on + strand at 2162.893 kb on - strand at 2162.909 kb on + strand at 2162.909 kb on + strand at 2162.910 kb on - strand at 2162.910 kb on - strand at 2162.910 kb on - strand at 2162.986 kb on + strand at 2163.141 kb on + strand, within Psyr_1893 at 2163.141 kb on + strand, within Psyr_1893 at 2163.141 kb on + strand, within Psyr_1893 at 2163.141 kb on + strand, within Psyr_1893 at 2163.141 kb on + strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.142 kb on - strand, within Psyr_1893 at 2163.466 kb on + strand, within Psyr_1893 at 2163.467 kb on - strand, within Psyr_1893 at 2163.551 kb on + strand at 2163.551 kb on + strand at 2163.619 kb on + strand at 2163.619 kb on + strand at 2163.619 kb on + strand at 2163.620 kb on - strand at 2163.796 kb on + strand at 2163.796 kb on + strand at 2163.796 kb on + strand at 2163.797 kb on - strand at 2163.797 kb on - strand at 2163.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 2,161,229 + +2.7 2,161,229 + +1.3 2,161,229 + +0.7 2,161,229 + -1.2 2,161,229 + +1.8 2,161,229 + +0.9 2,161,230 - +2.0 2,161,230 - -1.1 2,161,230 - -0.8 2,161,230 - +0.7 2,161,230 - +0.3 2,161,230 - +3.8 2,161,240 - +1.4 2,161,290 + +1.9 2,161,290 + +0.4 2,161,291 - +0.1 2,161,291 - +0.0 2,161,291 - +1.7 2,161,316 + +0.2 2,161,317 - +0.0 2,161,317 - -0.4 2,161,317 - -0.4 2,161,317 - +0.2 2,161,350 + -4.3 2,161,350 + -2.2 2,161,350 + -1.1 2,161,351 - +0.8 2,161,351 - -0.3 2,161,361 + +1.0 2,161,361 + +0.2 2,161,361 + +0.6 2,161,361 + +0.8 2,161,361 + +0.2 2,161,361 + -2.8 2,161,361 + -1.2 2,161,362 - +2.0 2,161,362 - -0.8 2,161,362 - +1.3 2,161,362 - +0.7 2,161,362 - -1.6 2,161,366 - -0.5 2,161,366 - +0.8 2,161,366 - -1.1 2,161,512 + Psyr_1891 0.37 -1.1 2,161,512 + Psyr_1891 0.37 +0.5 2,161,512 + Psyr_1891 0.37 -2.3 2,161,512 + Psyr_1891 0.37 +0.0 2,161,513 - Psyr_1891 0.38 -0.3 2,161,602 + Psyr_1891 0.67 +0.8 2,161,606 - Psyr_1891 0.68 -0.7 2,161,606 - Psyr_1891 0.68 +2.6 2,161,612 + Psyr_1891 0.70 -0.6 2,161,612 + Psyr_1891 0.70 +1.8 2,161,613 - Psyr_1891 0.71 +0.9 2,161,613 - Psyr_1891 0.71 -1.3 2,161,655 - Psyr_1891 0.84 -1.4 2,161,760 + +1.0 2,161,760 + -0.1 2,161,760 + -0.6 2,161,760 + +0.3 2,161,760 + +0.2 2,161,761 - +0.1 2,161,761 - +1.4 2,161,761 - +0.2 2,161,805 + -2.0 2,161,899 + -1.0 2,162,221 + -1.3 2,162,222 - +0.0 2,162,222 - +1.5 2,162,222 - -0.6 2,162,528 + Psyr_1892 0.47 +1.8 2,162,528 + Psyr_1892 0.47 +0.1 2,162,528 + Psyr_1892 0.47 -1.5 2,162,528 + Psyr_1892 0.47 +1.6 2,162,528 + Psyr_1892 0.47 -2.0 2,162,528 + Psyr_1892 0.47 +0.7 2,162,529 - Psyr_1892 0.47 -1.7 2,162,529 - Psyr_1892 0.47 -1.2 2,162,529 - Psyr_1892 0.47 -0.6 2,162,529 - Psyr_1892 0.47 +0.4 2,162,806 + Psyr_1892 0.84 +1.4 2,162,806 + Psyr_1892 0.84 -0.2 2,162,806 + Psyr_1892 0.84 -0.6 2,162,806 + Psyr_1892 0.84 -4.1 2,162,806 + Psyr_1892 0.84 +0.8 2,162,810 + Psyr_1892 0.85 -2.9 2,162,810 + Psyr_1892 0.85 -1.3 2,162,892 + +3.7 2,162,893 - +0.0 2,162,909 + +0.3 2,162,909 + -0.7 2,162,910 - -0.5 2,162,910 - +4.3 2,162,910 - +0.0 2,162,986 + +0.8 2,163,141 + Psyr_1893 0.18 +0.8 2,163,141 + Psyr_1893 0.18 -0.2 2,163,141 + Psyr_1893 0.18 +1.0 2,163,141 + Psyr_1893 0.18 +0.3 2,163,141 + Psyr_1893 0.18 +0.7 2,163,142 - Psyr_1893 0.18 +0.4 2,163,142 - Psyr_1893 0.18 +0.7 2,163,142 - Psyr_1893 0.18 -2.6 2,163,142 - Psyr_1893 0.18 +1.0 2,163,142 - Psyr_1893 0.18 -0.3 2,163,142 - Psyr_1893 0.18 +0.5 2,163,142 - Psyr_1893 0.18 +0.2 2,163,142 - Psyr_1893 0.18 +1.9 2,163,142 - Psyr_1893 0.18 -3.2 2,163,466 + Psyr_1893 0.75 +0.9 2,163,467 - Psyr_1893 0.76 +0.8 2,163,551 + +0.0 2,163,551 + -1.4 2,163,619 + +1.7 2,163,619 + -2.0 2,163,619 + -0.6 2,163,620 - -0.1 2,163,796 + +0.7 2,163,796 + +1.4 2,163,796 + -0.5 2,163,797 - +0.6 2,163,797 - +0.4 2,163,797 - -0.8
Or see this region's nucleotide sequence