Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1722 and Psyr_1723 are separated by 47 nucleotides Psyr_1723 and Psyr_1724 are separated by 367 nucleotides Psyr_1724 and Psyr_1725 are separated by 86 nucleotides Psyr_1725 and Psyr_1726 are separated by 314 nucleotides
Psyr_1722: Psyr_1722 - Amidohydrolase 2, at 1,951,605 to 1,952,351
_1722
Psyr_1723: Psyr_1723 - conserved hypothetical protein, at 1,952,399 to 1,952,758
_1723
Psyr_1724: Psyr_1724 - glutamate dehydrogenase (NAD), at 1,953,126 to 1,957,982
_1724
Psyr_1725: Psyr_1725 - Protein of unknown function DUF204, at 1,958,069 to 1,958,605
_1725
Psyr_1726: Psyr_1726 - aconitase, at 1,958,920 to 1,961,535
_1726
Position (kb) 1953
1954
1955
1956
1957
1958 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1952.174 kb on - strand, within Psyr_1722 at 1952.251 kb on + strand, within Psyr_1722 at 1952.418 kb on + strand at 1952.419 kb on - strand at 1952.419 kb on - strand at 1952.448 kb on - strand at 1952.709 kb on + strand, within Psyr_1723 at 1952.818 kb on + strand at 1952.818 kb on + strand at 1952.818 kb on + strand at 1952.844 kb on + strand at 1952.845 kb on - strand at 1952.854 kb on - strand at 1952.874 kb on + strand at 1952.874 kb on + strand at 1952.875 kb on - strand at 1952.875 kb on - strand at 1952.875 kb on - strand at 1952.875 kb on - strand at 1952.939 kb on + strand at 1952.939 kb on + strand at 1952.978 kb on + strand at 1952.978 kb on + strand at 1952.978 kb on + strand at 1952.979 kb on - strand at 1952.979 kb on - strand at 1952.979 kb on - strand at 1952.979 kb on - strand at 1953.013 kb on + strand at 1953.013 kb on + strand at 1953.014 kb on - strand at 1953.014 kb on - strand at 1953.014 kb on - strand at 1953.014 kb on - strand at 1953.014 kb on - strand at 1953.018 kb on + strand at 1953.018 kb on + strand at 1953.018 kb on + strand at 1953.018 kb on + strand at 1953.019 kb on - strand at 1953.019 kb on - strand at 1953.024 kb on + strand at 1953.024 kb on + strand at 1953.102 kb on - strand at 1953.119 kb on - strand at 1953.363 kb on + strand at 1953.376 kb on - strand at 1953.404 kb on + strand at 1953.405 kb on - strand at 1953.405 kb on - strand at 1953.405 kb on - strand at 1953.600 kb on + strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.601 kb on - strand at 1953.680 kb on - strand, within Psyr_1724 at 1953.885 kb on + strand, within Psyr_1724 at 1953.975 kb on + strand, within Psyr_1724 at 1953.975 kb on + strand, within Psyr_1724 at 1953.975 kb on + strand, within Psyr_1724 at 1953.975 kb on + strand, within Psyr_1724 at 1953.976 kb on - strand, within Psyr_1724 at 1954.054 kb on - strand, within Psyr_1724 at 1954.119 kb on + strand, within Psyr_1724 at 1954.119 kb on + strand, within Psyr_1724 at 1954.120 kb on - strand, within Psyr_1724 at 1954.120 kb on - strand, within Psyr_1724 at 1954.134 kb on + strand, within Psyr_1724 at 1954.161 kb on + strand, within Psyr_1724 at 1954.395 kb on + strand, within Psyr_1724 at 1954.395 kb on + strand, within Psyr_1724 at 1954.395 kb on + strand, within Psyr_1724 at 1954.395 kb on + strand, within Psyr_1724 at 1954.395 kb on + strand, within Psyr_1724 at 1954.396 kb on - strand, within Psyr_1724 at 1954.396 kb on - strand, within Psyr_1724 at 1954.396 kb on - strand, within Psyr_1724 at 1954.396 kb on - strand, within Psyr_1724 at 1954.396 kb on - strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.411 kb on + strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.412 kb on - strand, within Psyr_1724 at 1954.413 kb on + strand, within Psyr_1724 at 1954.413 kb on + strand, within Psyr_1724 at 1954.426 kb on + strand, within Psyr_1724 at 1954.426 kb on + strand, within Psyr_1724 at 1954.427 kb on - strand, within Psyr_1724 at 1954.795 kb on - strand, within Psyr_1724 at 1954.839 kb on + strand, within Psyr_1724 at 1954.839 kb on + strand, within Psyr_1724 at 1954.980 kb on + strand, within Psyr_1724 at 1955.090 kb on - strand, within Psyr_1724 at 1955.136 kb on + strand, within Psyr_1724 at 1955.181 kb on + strand, within Psyr_1724 at 1955.181 kb on + strand, within Psyr_1724 at 1955.254 kb on - strand, within Psyr_1724 at 1955.374 kb on - strand, within Psyr_1724 at 1955.374 kb on - strand, within Psyr_1724 at 1955.445 kb on + strand, within Psyr_1724 at 1955.446 kb on - strand, within Psyr_1724 at 1955.446 kb on - strand, within Psyr_1724 at 1955.446 kb on - strand, within Psyr_1724 at 1956.255 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.438 kb on + strand, within Psyr_1724 at 1956.439 kb on - strand, within Psyr_1724 at 1956.439 kb on - strand, within Psyr_1724 at 1956.774 kb on + strand, within Psyr_1724 at 1956.774 kb on + strand, within Psyr_1724 at 1956.775 kb on - strand, within Psyr_1724 at 1956.775 kb on - strand, within Psyr_1724 at 1956.775 kb on - strand, within Psyr_1724 at 1956.817 kb on - strand, within Psyr_1724 at 1956.817 kb on - strand, within Psyr_1724 at 1956.975 kb on + strand, within Psyr_1724 at 1956.976 kb on - strand, within Psyr_1724 at 1957.293 kb on + strand, within Psyr_1724 at 1957.294 kb on - strand, within Psyr_1724 at 1957.509 kb on + strand at 1957.510 kb on - strand at 1957.510 kb on - strand at 1957.510 kb on - strand at 1957.557 kb on - strand at 1957.569 kb on + strand at 1957.569 kb on + strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.570 kb on - strand at 1957.571 kb on + strand at 1957.571 kb on + strand at 1957.572 kb on - strand at 1957.572 kb on - strand at 1957.638 kb on + strand at 1957.638 kb on + strand at 1957.638 kb on + strand at 1957.667 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.981 kb on - strand at 1957.992 kb on - strand at 1957.992 kb on - strand at 1958.006 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.246 kb on - strand, within Psyr_1725 at 1958.270 kb on + strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.517 kb on - strand, within Psyr_1725 at 1958.554 kb on + strand at 1958.554 kb on + strand at 1958.555 kb on - strand at 1958.645 kb on + strand at 1958.646 kb on - strand at 1958.647 kb on + strand at 1958.822 kb on + strand at 1958.822 kb on + strand at 1958.823 kb on - strand at 1958.823 kb on - strand at 1958.824 kb on + strand at 1958.825 kb on - strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.847 kb on + strand at 1958.847 kb on + strand at 1958.847 kb on + strand at 1958.848 kb on - strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.852 kb on - strand at 1958.917 kb on + strand at 1958.918 kb on - strand at 1958.921 kb on + strand at 1958.921 kb on + strand at 1958.922 kb on - strand at 1958.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 1,952,174 - Psyr_1722 0.76 -1.7 1,952,251 + Psyr_1722 0.86 -0.5 1,952,418 + -0.2 1,952,419 - -0.0 1,952,419 - -1.0 1,952,448 - -1.5 1,952,709 + Psyr_1723 0.86 +2.2 1,952,818 + -1.1 1,952,818 + +0.1 1,952,818 + -1.0 1,952,844 + -1.2 1,952,845 - -1.1 1,952,854 - +0.3 1,952,874 + +0.7 1,952,874 + -3.3 1,952,875 - +0.6 1,952,875 - -2.0 1,952,875 - -0.1 1,952,875 - -2.3 1,952,939 + -2.0 1,952,939 + -0.1 1,952,978 + +0.1 1,952,978 + +0.5 1,952,978 + +2.7 1,952,979 - -1.4 1,952,979 - -2.6 1,952,979 - -2.6 1,952,979 - -0.9 1,953,013 + +0.2 1,953,013 + +1.8 1,953,014 - -1.2 1,953,014 - -0.1 1,953,014 - +1.1 1,953,014 - -2.6 1,953,014 - +0.1 1,953,018 + -0.7 1,953,018 + -0.5 1,953,018 + -0.5 1,953,018 + -0.3 1,953,019 - -0.1 1,953,019 - -4.1 1,953,024 + -0.7 1,953,024 + +0.7 1,953,102 - -2.8 1,953,119 - +1.1 1,953,363 + -0.3 1,953,376 - -0.5 1,953,404 + -2.9 1,953,405 - -0.7 1,953,405 - -0.8 1,953,405 - -0.1 1,953,600 + +0.8 1,953,601 - -1.6 1,953,601 - -1.7 1,953,601 - +1.1 1,953,601 - -2.5 1,953,601 - -1.3 1,953,601 - -2.6 1,953,601 - +1.1 1,953,680 - Psyr_1724 0.11 +0.7 1,953,885 + Psyr_1724 0.16 -3.8 1,953,975 + Psyr_1724 0.17 +0.1 1,953,975 + Psyr_1724 0.17 -1.5 1,953,975 + Psyr_1724 0.17 -0.1 1,953,975 + Psyr_1724 0.17 -1.4 1,953,976 - Psyr_1724 0.18 -1.9 1,954,054 - Psyr_1724 0.19 +0.2 1,954,119 + Psyr_1724 0.20 -3.6 1,954,119 + Psyr_1724 0.20 -1.1 1,954,120 - Psyr_1724 0.20 -2.1 1,954,120 - Psyr_1724 0.20 -0.1 1,954,134 + Psyr_1724 0.21 +0.1 1,954,161 + Psyr_1724 0.21 -1.5 1,954,395 + Psyr_1724 0.26 +1.1 1,954,395 + Psyr_1724 0.26 +1.1 1,954,395 + Psyr_1724 0.26 -2.5 1,954,395 + Psyr_1724 0.26 -1.2 1,954,395 + Psyr_1724 0.26 -1.1 1,954,396 - Psyr_1724 0.26 -0.8 1,954,396 - Psyr_1724 0.26 -2.7 1,954,396 - Psyr_1724 0.26 -0.9 1,954,396 - Psyr_1724 0.26 -0.1 1,954,396 - Psyr_1724 0.26 +2.1 1,954,411 + Psyr_1724 0.26 -3.3 1,954,411 + Psyr_1724 0.26 +0.6 1,954,411 + Psyr_1724 0.26 -0.2 1,954,411 + Psyr_1724 0.26 -1.4 1,954,411 + Psyr_1724 0.26 -4.0 1,954,411 + Psyr_1724 0.26 -1.4 1,954,411 + Psyr_1724 0.26 -1.8 1,954,411 + Psyr_1724 0.26 -0.0 1,954,411 + Psyr_1724 0.26 -4.1 1,954,412 - Psyr_1724 0.26 +1.9 1,954,412 - Psyr_1724 0.26 +3.3 1,954,412 - Psyr_1724 0.26 -4.6 1,954,412 - Psyr_1724 0.26 -0.7 1,954,412 - Psyr_1724 0.26 -0.5 1,954,412 - Psyr_1724 0.26 -1.7 1,954,412 - Psyr_1724 0.26 +0.5 1,954,412 - Psyr_1724 0.26 -2.1 1,954,412 - Psyr_1724 0.26 -2.3 1,954,413 + Psyr_1724 0.26 -1.2 1,954,413 + Psyr_1724 0.26 +0.3 1,954,426 + Psyr_1724 0.27 +0.5 1,954,426 + Psyr_1724 0.27 -2.1 1,954,427 - Psyr_1724 0.27 +0.1 1,954,795 - Psyr_1724 0.34 -2.4 1,954,839 + Psyr_1724 0.35 -2.6 1,954,839 + Psyr_1724 0.35 -2.2 1,954,980 + Psyr_1724 0.38 -1.1 1,955,090 - Psyr_1724 0.40 +0.1 1,955,136 + Psyr_1724 0.41 -3.0 1,955,181 + Psyr_1724 0.42 -0.3 1,955,181 + Psyr_1724 0.42 -0.6 1,955,254 - Psyr_1724 0.44 -1.5 1,955,374 - Psyr_1724 0.46 -0.8 1,955,374 - Psyr_1724 0.46 -3.7 1,955,445 + Psyr_1724 0.48 -0.4 1,955,446 - Psyr_1724 0.48 -2.2 1,955,446 - Psyr_1724 0.48 -2.5 1,955,446 - Psyr_1724 0.48 -2.8 1,956,255 + Psyr_1724 0.64 +0.0 1,956,438 + Psyr_1724 0.68 -2.2 1,956,438 + Psyr_1724 0.68 -0.2 1,956,438 + Psyr_1724 0.68 -1.7 1,956,438 + Psyr_1724 0.68 -3.1 1,956,438 + Psyr_1724 0.68 -0.5 1,956,438 + Psyr_1724 0.68 -1.1 1,956,438 + Psyr_1724 0.68 -2.0 1,956,438 + Psyr_1724 0.68 -1.3 1,956,438 + Psyr_1724 0.68 -0.8 1,956,439 - Psyr_1724 0.68 -3.3 1,956,439 - Psyr_1724 0.68 +0.9 1,956,774 + Psyr_1724 0.75 -0.9 1,956,774 + Psyr_1724 0.75 -0.6 1,956,775 - Psyr_1724 0.75 -3.3 1,956,775 - Psyr_1724 0.75 +0.6 1,956,775 - Psyr_1724 0.75 +1.6 1,956,817 - Psyr_1724 0.76 -1.9 1,956,817 - Psyr_1724 0.76 -1.2 1,956,975 + Psyr_1724 0.79 -4.6 1,956,976 - Psyr_1724 0.79 +1.5 1,957,293 + Psyr_1724 0.86 -1.4 1,957,294 - Psyr_1724 0.86 -2.2 1,957,509 + -0.2 1,957,510 - -0.9 1,957,510 - -0.1 1,957,510 - -0.5 1,957,557 - -2.5 1,957,569 + -1.8 1,957,569 + -0.5 1,957,570 - -1.9 1,957,570 - -0.4 1,957,570 - -1.2 1,957,570 - -1.6 1,957,570 - -2.4 1,957,571 + -2.1 1,957,571 + -2.4 1,957,572 - +1.1 1,957,572 - -0.7 1,957,638 + -1.2 1,957,638 + +0.1 1,957,638 + +2.1 1,957,667 + -1.9 1,957,920 + -2.1 1,957,920 + -1.7 1,957,920 + -1.5 1,957,920 + +3.7 1,957,920 + +1.5 1,957,920 + -1.3 1,957,920 + +1.2 1,957,920 + +0.8 1,957,921 - -0.0 1,957,921 - -0.9 1,957,921 - +2.1 1,957,921 - +0.1 1,957,921 - +0.0 1,957,921 - -0.9 1,957,921 - +0.6 1,957,981 - -2.5 1,957,992 - -2.5 1,957,992 - +0.3 1,958,006 + +0.7 1,958,052 + -2.2 1,958,052 + +0.4 1,958,052 + -0.0 1,958,052 + -0.9 1,958,246 - Psyr_1725 0.33 -2.3 1,958,270 + Psyr_1725 0.37 -0.5 1,958,386 - Psyr_1725 0.59 +0.2 1,958,517 - Psyr_1725 0.83 -2.3 1,958,517 - Psyr_1725 0.83 -2.2 1,958,517 - Psyr_1725 0.83 +0.6 1,958,554 + -0.4 1,958,554 + +0.9 1,958,555 - -0.2 1,958,645 + -0.7 1,958,646 - +0.3 1,958,647 + +0.4 1,958,822 + +0.7 1,958,822 + -0.2 1,958,823 - -0.3 1,958,823 - +0.1 1,958,824 + +0.7 1,958,825 - -0.3 1,958,845 + +0.3 1,958,845 + -1.6 1,958,845 + -0.6 1,958,845 + -0.7 1,958,845 + +0.4 1,958,846 - +1.1 1,958,846 - +0.0 1,958,846 - +0.1 1,958,846 - +0.7 1,958,847 + -2.1 1,958,847 + -1.8 1,958,847 + +1.1 1,958,848 - -1.6 1,958,851 + +2.1 1,958,851 + +2.7 1,958,851 + -0.5 1,958,852 - -1.2 1,958,852 - +0.5 1,958,852 - -1.3 1,958,852 - +1.1 1,958,917 + -3.6 1,958,918 - -1.8 1,958,921 + -2.5 1,958,921 + -1.7 1,958,922 - +0.8 1,958,922 - +1.1
Or see this region's nucleotide sequence