Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1639 and Psyr_1640 overlap by 11 nucleotides Psyr_1640 and Psyr_1641 are separated by 24 nucleotides Psyr_1641 and Psyr_1642 are separated by 55 nucleotides Psyr_1642 and Psyr_1643 are separated by 31 nucleotides
Psyr_1639: Psyr_1639 - ribosomal large subunit pseudouridine synthase C, at 1,846,318 to 1,847,274
_1639
Psyr_1640: Psyr_1640 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 1,847,264 to 1,847,926
_1640
Psyr_1641: Psyr_1641 - Peptidase S49, SppA, at 1,847,951 to 1,848,949
_1641
Psyr_1642: Psyr_1642 - Maf-like protein, at 1,849,005 to 1,849,658
_1642
Psyr_1643: Psyr_1643 - Protein of unknown function DUF177, at 1,849,690 to 1,850,217
_1643
Position (kb)
1847
1848
1849 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1847.090 kb on + strand, within Psyr_1639 at 1847.107 kb on + strand, within Psyr_1639 at 1847.107 kb on + strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.211 kb on + strand at 1847.212 kb on - strand at 1847.212 kb on - strand at 1847.277 kb on - strand at 1847.486 kb on + strand, within Psyr_1640 at 1847.487 kb on - strand, within Psyr_1640 at 1847.487 kb on - strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.651 kb on + strand, within Psyr_1640 at 1847.651 kb on + strand, within Psyr_1640 at 1847.652 kb on - strand, within Psyr_1640 at 1847.652 kb on - strand, within Psyr_1640 at 1847.652 kb on - strand at 1848.019 kb on + strand at 1848.019 kb on + strand at 1848.020 kb on - strand at 1848.020 kb on - strand at 1848.345 kb on - strand, within Psyr_1641 at 1848.350 kb on + strand, within Psyr_1641 at 1848.351 kb on - strand, within Psyr_1641 at 1848.398 kb on + strand, within Psyr_1641 at 1848.398 kb on + strand, within Psyr_1641 at 1848.399 kb on - strand, within Psyr_1641 at 1848.399 kb on - strand, within Psyr_1641 at 1848.434 kb on + strand, within Psyr_1641 at 1848.434 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.437 kb on + strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.438 kb on - strand, within Psyr_1641 at 1848.464 kb on + strand, within Psyr_1641 at 1848.464 kb on + strand, within Psyr_1641 at 1848.464 kb on + strand, within Psyr_1641 at 1848.465 kb on - strand, within Psyr_1641 at 1848.465 kb on - strand, within Psyr_1641 at 1848.559 kb on + strand, within Psyr_1641 at 1848.559 kb on + strand, within Psyr_1641 at 1848.560 kb on - strand, within Psyr_1641 at 1848.560 kb on - strand, within Psyr_1641 at 1848.560 kb on - strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.563 kb on + strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.564 kb on - strand, within Psyr_1641 at 1848.565 kb on + strand, within Psyr_1641 at 1848.565 kb on + strand, within Psyr_1641 at 1848.565 kb on + strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.801 kb on - strand, within Psyr_1641 at 1848.842 kb on + strand, within Psyr_1641 at 1848.842 kb on + strand, within Psyr_1641 at 1848.964 kb on + strand at 1848.964 kb on + strand at 1848.964 kb on + strand at 1848.964 kb on + strand at 1848.965 kb on - strand at 1848.966 kb on + strand at 1848.966 kb on + strand at 1848.966 kb on + strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1848.967 kb on - strand at 1849.029 kb on + strand at 1849.029 kb on + strand at 1849.032 kb on + strand at 1849.032 kb on + strand at 1849.032 kb on + strand at 1849.032 kb on + strand at 1849.033 kb on - strand at 1849.033 kb on - strand at 1849.033 kb on - strand at 1849.050 kb on + strand at 1849.051 kb on - strand at 1849.051 kb on - strand at 1849.150 kb on + strand, within Psyr_1642 at 1849.214 kb on + strand, within Psyr_1642 at 1849.214 kb on + strand, within Psyr_1642 at 1849.215 kb on - strand, within Psyr_1642 at 1849.215 kb on - strand, within Psyr_1642 at 1849.449 kb on + strand, within Psyr_1642 at 1849.450 kb on - strand, within Psyr_1642 at 1849.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 1,847,090 + Psyr_1639 0.81 +1.2 1,847,107 + Psyr_1639 0.82 +2.4 1,847,107 + Psyr_1639 0.82 +3.2 1,847,108 - Psyr_1639 0.83 +0.5 1,847,108 - Psyr_1639 0.83 -0.2 1,847,108 - Psyr_1639 0.83 +1.2 1,847,173 + Psyr_1639 0.89 -2.0 1,847,173 + Psyr_1639 0.89 +1.9 1,847,173 + Psyr_1639 0.89 +0.5 1,847,173 + Psyr_1639 0.89 +0.2 1,847,173 + Psyr_1639 0.89 +0.2 1,847,174 - Psyr_1639 0.89 -0.7 1,847,174 - Psyr_1639 0.89 +1.0 1,847,174 - Psyr_1639 0.89 +1.3 1,847,174 - Psyr_1639 0.89 -2.3 1,847,174 - Psyr_1639 0.89 -0.2 1,847,174 - Psyr_1639 0.89 +0.3 1,847,174 - Psyr_1639 0.89 +2.4 1,847,174 - Psyr_1639 0.89 -0.0 1,847,174 - Psyr_1639 0.89 +0.5 1,847,174 - Psyr_1639 0.89 -0.7 1,847,174 - Psyr_1639 0.89 +1.2 1,847,174 - Psyr_1639 0.89 +2.0 1,847,174 - Psyr_1639 0.89 -0.0 1,847,174 - Psyr_1639 0.89 -0.2 1,847,174 - Psyr_1639 0.89 -2.5 1,847,174 - Psyr_1639 0.89 -2.2 1,847,211 + +0.9 1,847,212 - -0.7 1,847,212 - -1.0 1,847,277 - +1.1 1,847,486 + Psyr_1640 0.33 -1.4 1,847,487 - Psyr_1640 0.34 +1.8 1,847,487 - Psyr_1640 0.34 +1.1 1,847,498 + Psyr_1640 0.35 +0.5 1,847,498 + Psyr_1640 0.35 +0.1 1,847,498 + Psyr_1640 0.35 +0.6 1,847,498 + Psyr_1640 0.35 -0.2 1,847,498 + Psyr_1640 0.35 +0.2 1,847,498 + Psyr_1640 0.35 -2.7 1,847,498 + Psyr_1640 0.35 +0.9 1,847,498 + Psyr_1640 0.35 +1.4 1,847,498 + Psyr_1640 0.35 -1.1 1,847,498 + Psyr_1640 0.35 -2.3 1,847,498 + Psyr_1640 0.35 +0.2 1,847,498 + Psyr_1640 0.35 -0.9 1,847,498 + Psyr_1640 0.35 +0.6 1,847,498 + Psyr_1640 0.35 +1.8 1,847,498 + Psyr_1640 0.35 +0.4 1,847,499 - Psyr_1640 0.35 +1.1 1,847,499 - Psyr_1640 0.35 +1.0 1,847,499 - Psyr_1640 0.35 +0.4 1,847,499 - Psyr_1640 0.35 -0.2 1,847,499 - Psyr_1640 0.35 -0.4 1,847,499 - Psyr_1640 0.35 -1.9 1,847,651 + Psyr_1640 0.58 -1.4 1,847,651 + Psyr_1640 0.58 -0.3 1,847,652 - Psyr_1640 0.59 +1.3 1,847,652 - Psyr_1640 0.59 -0.9 1,847,652 - -1.4 1,848,019 + -3.8 1,848,019 + +0.5 1,848,020 - +0.8 1,848,020 - +0.2 1,848,345 - Psyr_1641 0.39 +0.3 1,848,350 + Psyr_1641 0.40 +0.1 1,848,351 - Psyr_1641 0.40 +0.6 1,848,398 + Psyr_1641 0.45 +2.0 1,848,398 + Psyr_1641 0.45 -0.0 1,848,399 - Psyr_1641 0.45 +1.1 1,848,399 - Psyr_1641 0.45 +0.1 1,848,434 + Psyr_1641 0.48 -0.4 1,848,434 + Psyr_1641 0.48 -1.0 1,848,437 + Psyr_1641 0.49 +1.1 1,848,437 + Psyr_1641 0.49 -1.7 1,848,437 + Psyr_1641 0.49 +2.6 1,848,437 + Psyr_1641 0.49 +0.1 1,848,437 + Psyr_1641 0.49 -3.0 1,848,437 + Psyr_1641 0.49 +0.5 1,848,437 + Psyr_1641 0.49 +3.1 1,848,437 + Psyr_1641 0.49 +0.6 1,848,437 + Psyr_1641 0.49 -1.0 1,848,437 + Psyr_1641 0.49 -2.6 1,848,437 + Psyr_1641 0.49 -1.0 1,848,438 - Psyr_1641 0.49 +0.9 1,848,438 - Psyr_1641 0.49 +0.7 1,848,438 - Psyr_1641 0.49 -0.1 1,848,438 - Psyr_1641 0.49 -0.9 1,848,438 - Psyr_1641 0.49 +2.2 1,848,438 - Psyr_1641 0.49 +0.3 1,848,464 + Psyr_1641 0.51 +0.8 1,848,464 + Psyr_1641 0.51 +0.5 1,848,464 + Psyr_1641 0.51 +0.9 1,848,465 - Psyr_1641 0.51 -1.9 1,848,465 - Psyr_1641 0.51 -1.9 1,848,559 + Psyr_1641 0.61 -0.7 1,848,559 + Psyr_1641 0.61 -2.0 1,848,560 - Psyr_1641 0.61 +0.9 1,848,560 - Psyr_1641 0.61 +0.9 1,848,560 - Psyr_1641 0.61 -2.2 1,848,563 + Psyr_1641 0.61 +0.7 1,848,563 + Psyr_1641 0.61 +1.3 1,848,563 + Psyr_1641 0.61 +2.1 1,848,563 + Psyr_1641 0.61 +1.3 1,848,563 + Psyr_1641 0.61 +0.2 1,848,563 + Psyr_1641 0.61 +0.2 1,848,563 + Psyr_1641 0.61 +0.3 1,848,563 + Psyr_1641 0.61 -2.4 1,848,563 + Psyr_1641 0.61 +2.0 1,848,564 - Psyr_1641 0.61 -2.2 1,848,564 - Psyr_1641 0.61 +0.3 1,848,564 - Psyr_1641 0.61 +1.5 1,848,564 - Psyr_1641 0.61 -1.0 1,848,564 - Psyr_1641 0.61 -2.9 1,848,564 - Psyr_1641 0.61 +2.0 1,848,565 + Psyr_1641 0.61 -0.6 1,848,565 + Psyr_1641 0.61 -0.6 1,848,565 + Psyr_1641 0.61 +0.9 1,848,801 - Psyr_1641 0.85 +0.6 1,848,801 - Psyr_1641 0.85 +0.1 1,848,801 - Psyr_1641 0.85 +1.6 1,848,801 - Psyr_1641 0.85 -0.3 1,848,801 - Psyr_1641 0.85 +1.2 1,848,801 - Psyr_1641 0.85 -2.4 1,848,801 - Psyr_1641 0.85 +0.3 1,848,842 + Psyr_1641 0.89 -0.5 1,848,842 + Psyr_1641 0.89 -0.1 1,848,964 + -2.6 1,848,964 + +3.0 1,848,964 + -0.3 1,848,964 + -0.6 1,848,965 - -1.2 1,848,966 + +0.7 1,848,966 + -0.4 1,848,966 + -2.0 1,848,967 - +1.1 1,848,967 - -2.7 1,848,967 - -1.4 1,848,967 - -1.9 1,848,967 - +0.1 1,848,967 - +0.4 1,848,967 - +0.6 1,849,029 + -1.2 1,849,029 + +1.5 1,849,032 + +1.9 1,849,032 + -1.0 1,849,032 + +0.6 1,849,032 + +0.7 1,849,033 - -0.1 1,849,033 - +0.8 1,849,033 - +0.5 1,849,050 + -0.7 1,849,051 - +0.7 1,849,051 - +1.1 1,849,150 + Psyr_1642 0.22 +1.1 1,849,214 + Psyr_1642 0.32 -1.0 1,849,214 + Psyr_1642 0.32 -1.7 1,849,215 - Psyr_1642 0.32 +0.5 1,849,215 - Psyr_1642 0.32 -1.0 1,849,449 + Psyr_1642 0.68 +0.9 1,849,450 - Psyr_1642 0.68 -2.0 1,849,614 - +2.0
Or see this region's nucleotide sequence