Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1620 and Psyr_1621 are separated by 219 nucleotides Psyr_1621 and Psyr_1622 are separated by 1 nucleotides Psyr_1622 and Psyr_1623 are separated by 287 nucleotides
Psyr_1620: Psyr_1620 - conserved hypothetical protein, at 1,821,315 to 1,822,511
_1620
Psyr_1621: Psyr_1621 - Autoinducer synthesis protein, at 1,822,731 to 1,823,411
_1621
Psyr_1622: Psyr_1622 - regulatory protein, LuxR:Autoinducer-binding protein, at 1,823,413 to 1,824,156
_1622
Psyr_1623: Psyr_1623 - Pyruvate dehydrogenase (lipoamide), at 1,824,444 to 1,825,547
_1623
Position (kb) 1823
1824
1825 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 1822.455 kb on - strand at 1822.499 kb on - strand at 1822.499 kb on - strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.505 kb on + strand at 1822.506 kb on - strand at 1822.506 kb on - strand at 1822.611 kb on + strand at 1822.611 kb on + strand at 1822.612 kb on - strand at 1822.612 kb on - strand at 1822.615 kb on + strand at 1822.615 kb on + strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.616 kb on - strand at 1822.663 kb on + strand at 1822.667 kb on + strand at 1822.673 kb on - strand at 1822.691 kb on - strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.694 kb on + strand at 1822.727 kb on + strand at 1822.728 kb on - strand at 1822.764 kb on + strand at 1822.764 kb on + strand at 1822.800 kb on + strand, within Psyr_1621 at 1822.801 kb on - strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.878 kb on + strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.879 kb on - strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.899 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1822.965 kb on + strand, within Psyr_1621 at 1823.255 kb on - strand, within Psyr_1621 at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.427 kb on + strand at 1823.428 kb on - strand at 1823.431 kb on - strand at 1823.441 kb on + strand at 1823.441 kb on + strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.442 kb on - strand at 1823.546 kb on + strand, within Psyr_1622 at 1823.582 kb on - strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.810 kb on + strand, within Psyr_1622 at 1823.811 kb on - strand, within Psyr_1622 at 1823.895 kb on + strand, within Psyr_1622 at 1823.896 kb on - strand, within Psyr_1622 at 1823.925 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.933 kb on + strand, within Psyr_1622 at 1823.934 kb on - strand, within Psyr_1622 at 1823.934 kb on - strand, within Psyr_1622 at 1823.947 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.949 kb on - strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1823.972 kb on + strand, within Psyr_1622 at 1824.023 kb on + strand, within Psyr_1622 at 1824.024 kb on - strand, within Psyr_1622 at 1824.024 kb on - strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.029 kb on + strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.030 kb on - strand, within Psyr_1622 at 1824.046 kb on - strand, within Psyr_1622 at 1824.144 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.158 kb on + strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.159 kb on - strand at 1824.161 kb on + strand at 1824.162 kb on - strand at 1824.162 kb on - strand at 1824.162 kb on - strand at 1824.170 kb on + strand at 1824.170 kb on + strand at 1824.171 kb on - strand at 1824.225 kb on + strand at 1824.286 kb on + strand at 1824.286 kb on + strand at 1824.374 kb on - strand at 1824.390 kb on + strand at 1824.390 kb on + strand at 1824.390 kb on + strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.391 kb on - strand at 1824.439 kb on + strand at 1824.439 kb on + strand at 1824.439 kb on + strand at 1824.440 kb on - strand at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.732 kb on + strand, within Psyr_1623 at 1824.733 kb on - strand, within Psyr_1623 at 1824.957 kb on + strand, within Psyr_1623 at 1824.958 kb on - strand, within Psyr_1623 at 1824.958 kb on - strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.981 kb on + strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.982 kb on - strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.984 kb on + strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1824.985 kb on - strand, within Psyr_1623 at 1825.108 kb on + strand, within Psyr_1623
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 1,822,455 - +0.9 1,822,499 - -2.0 1,822,499 - -0.9 1,822,505 + +0.8 1,822,505 + -0.2 1,822,505 + +0.8 1,822,506 - -1.4 1,822,506 - -0.1 1,822,611 + -0.4 1,822,611 + +1.2 1,822,612 - +2.4 1,822,612 - +0.8 1,822,615 + +0.1 1,822,615 + +0.1 1,822,616 - +1.2 1,822,616 - +3.5 1,822,616 - +2.0 1,822,663 + +1.6 1,822,667 + +2.1 1,822,673 - -0.2 1,822,691 - -1.2 1,822,694 + -0.2 1,822,694 + -0.1 1,822,694 + +0.8 1,822,694 + -1.0 1,822,727 + +0.4 1,822,728 - -1.8 1,822,764 + -1.1 1,822,764 + +2.6 1,822,800 + Psyr_1621 0.10 -1.8 1,822,801 - Psyr_1621 0.10 +0.2 1,822,878 + Psyr_1621 0.22 -0.8 1,822,878 + Psyr_1621 0.22 -1.2 1,822,878 + Psyr_1621 0.22 +0.0 1,822,878 + Psyr_1621 0.22 +2.0 1,822,879 - Psyr_1621 0.22 +1.4 1,822,879 - Psyr_1621 0.22 -0.8 1,822,899 + Psyr_1621 0.25 -0.6 1,822,899 + Psyr_1621 0.25 -1.4 1,822,965 + Psyr_1621 0.34 +1.2 1,822,965 + Psyr_1621 0.34 +0.2 1,823,255 - Psyr_1621 0.77 -2.2 1,823,427 + +0.2 1,823,427 + -0.6 1,823,427 + -2.4 1,823,428 - +2.0 1,823,431 - +0.2 1,823,441 + +0.6 1,823,441 + +0.5 1,823,442 - +2.5 1,823,442 - -0.4 1,823,442 - +0.4 1,823,442 - -2.2 1,823,546 + Psyr_1622 0.18 -0.7 1,823,582 - Psyr_1622 0.23 +2.6 1,823,810 + Psyr_1622 0.53 +0.9 1,823,810 + Psyr_1622 0.53 -1.8 1,823,810 + Psyr_1622 0.53 -0.6 1,823,810 + Psyr_1622 0.53 -0.9 1,823,811 - Psyr_1622 0.53 +0.7 1,823,895 + Psyr_1622 0.65 -2.3 1,823,896 - Psyr_1622 0.65 -1.8 1,823,925 + Psyr_1622 0.69 -3.6 1,823,933 + Psyr_1622 0.70 +0.8 1,823,933 + Psyr_1622 0.70 -0.5 1,823,933 + Psyr_1622 0.70 -1.4 1,823,933 + Psyr_1622 0.70 -1.6 1,823,934 - Psyr_1622 0.70 +1.1 1,823,934 - Psyr_1622 0.70 +0.4 1,823,947 - Psyr_1622 0.72 -1.5 1,823,949 - Psyr_1622 0.72 -2.4 1,823,949 - Psyr_1622 0.72 +0.6 1,823,949 - Psyr_1622 0.72 +0.2 1,823,949 - Psyr_1622 0.72 -0.0 1,823,972 + Psyr_1622 0.75 -0.1 1,823,972 + Psyr_1622 0.75 -0.0 1,823,972 + Psyr_1622 0.75 -1.6 1,824,023 + Psyr_1622 0.82 -0.3 1,824,024 - Psyr_1622 0.82 +0.8 1,824,024 - Psyr_1622 0.82 +0.4 1,824,029 + Psyr_1622 0.83 -2.3 1,824,029 + Psyr_1622 0.83 +0.6 1,824,029 + Psyr_1622 0.83 +0.7 1,824,029 + Psyr_1622 0.83 +1.2 1,824,030 - Psyr_1622 0.83 -1.0 1,824,030 - Psyr_1622 0.83 +2.2 1,824,030 - Psyr_1622 0.83 +0.1 1,824,030 - Psyr_1622 0.83 -4.2 1,824,046 - Psyr_1622 0.85 -0.4 1,824,144 + +0.5 1,824,158 + -0.6 1,824,158 + -0.2 1,824,158 + +2.8 1,824,158 + +0.5 1,824,158 + +1.0 1,824,158 + -0.2 1,824,158 + +1.4 1,824,158 + +1.2 1,824,159 - -2.6 1,824,159 - +2.0 1,824,159 - +1.5 1,824,159 - -0.0 1,824,159 - +1.2 1,824,161 + +0.0 1,824,162 - -1.4 1,824,162 - +0.9 1,824,162 - -0.6 1,824,170 + +3.8 1,824,170 + -0.6 1,824,171 - -0.2 1,824,225 + -0.1 1,824,286 + -0.3 1,824,286 + -1.4 1,824,374 - +0.1 1,824,390 + -0.6 1,824,390 + +1.4 1,824,390 + -0.4 1,824,391 - -1.7 1,824,391 - +1.2 1,824,391 - +0.0 1,824,391 - +0.3 1,824,439 + +1.3 1,824,439 + +0.8 1,824,439 + -2.7 1,824,440 - -0.3 1,824,732 + Psyr_1623 0.26 +1.3 1,824,732 + Psyr_1623 0.26 +0.4 1,824,732 + Psyr_1623 0.26 +4.0 1,824,732 + Psyr_1623 0.26 -0.2 1,824,733 - Psyr_1623 0.26 -0.1 1,824,957 + Psyr_1623 0.46 -0.0 1,824,958 - Psyr_1623 0.47 -1.0 1,824,958 - Psyr_1623 0.47 -0.8 1,824,981 + Psyr_1623 0.49 +0.9 1,824,981 + Psyr_1623 0.49 -1.7 1,824,981 + Psyr_1623 0.49 -0.5 1,824,982 - Psyr_1623 0.49 +0.3 1,824,982 - Psyr_1623 0.49 +1.0 1,824,982 - Psyr_1623 0.49 -0.0 1,824,984 + Psyr_1623 0.49 -1.0 1,824,984 + Psyr_1623 0.49 -0.1 1,824,984 + Psyr_1623 0.49 -1.8 1,824,984 + Psyr_1623 0.49 +0.3 1,824,984 + Psyr_1623 0.49 -0.7 1,824,984 + Psyr_1623 0.49 -0.3 1,824,984 + Psyr_1623 0.49 +3.9 1,824,984 + Psyr_1623 0.49 -0.3 1,824,984 + Psyr_1623 0.49 +0.8 1,824,985 - Psyr_1623 0.49 -0.7 1,824,985 - Psyr_1623 0.49 +1.6 1,824,985 - Psyr_1623 0.49 -0.5 1,824,985 - Psyr_1623 0.49 +0.7 1,824,985 - Psyr_1623 0.49 -0.4 1,824,985 - Psyr_1623 0.49 +0.9 1,824,985 - Psyr_1623 0.49 +0.3 1,824,985 - Psyr_1623 0.49 -0.4 1,824,985 - Psyr_1623 0.49 +1.0 1,824,985 - +1.2 1,824,985 - Psyr_1623 0.49 +0.5 1,824,985 - Psyr_1623 0.49 -0.4 1,824,985 - Psyr_1623 0.49 -0.6 1,824,985 - Psyr_1623 0.49 +3.2 1,825,108 + Psyr_1623 0.60 +2.2
Or see this region's nucleotide sequence