Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1234 and Psyr_1235 overlap by 1 nucleotides Psyr_1235 and Psyr_1236 are separated by 159 nucleotides Psyr_1236 and Psyr_1237 are separated by 44 nucleotides
Psyr_1234: Psyr_1234 - RNA methyltransferase TrmH, group 1, at 1,392,536 to 1,393,300
_1234
Psyr_1235: Psyr_1235 - serine O-acetyltransferase, at 1,393,300 to 1,394,076
_1235
Psyr_1236: Psyr_1236 - transcriptional regulator, BadM/Rrf2 family, at 1,394,236 to 1,394,727
_1236
Psyr_1237: Psyr_1237 - cysteine desulfurase IscS, at 1,394,772 to 1,395,986
_1237
Position (kb) 1394
1395 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1393.280 kb on + strand at 1393.280 kb on + strand at 1393.281 kb on - strand at 1393.281 kb on - strand at 1393.281 kb on - strand at 1393.281 kb on - strand at 1393.694 kb on + strand, within Psyr_1235 at 1393.694 kb on + strand, within Psyr_1235 at 1393.694 kb on + strand, within Psyr_1235 at 1393.695 kb on - strand, within Psyr_1235 at 1393.695 kb on - strand, within Psyr_1235 at 1393.695 kb on - strand, within Psyr_1235 at 1393.887 kb on + strand, within Psyr_1235 at 1394.105 kb on + strand at 1394.105 kb on + strand at 1394.106 kb on - strand at 1394.121 kb on + strand at 1394.122 kb on - strand at 1394.200 kb on + strand at 1394.200 kb on + strand at 1394.200 kb on + strand at 1394.247 kb on + strand at 1394.247 kb on + strand at 1394.343 kb on + strand, within Psyr_1236 at 1394.343 kb on + strand, within Psyr_1236 at 1394.356 kb on + strand, within Psyr_1236 at 1394.639 kb on + strand, within Psyr_1236 at 1394.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 1,393,280 + -0.8 1,393,280 + -2.1 1,393,281 - +0.3 1,393,281 - +0.0 1,393,281 - +0.7 1,393,281 - +2.0 1,393,694 + Psyr_1235 0.51 +0.3 1,393,694 + Psyr_1235 0.51 -0.9 1,393,694 + Psyr_1235 0.51 +0.0 1,393,695 - Psyr_1235 0.51 -0.6 1,393,695 - Psyr_1235 0.51 +0.9 1,393,695 - Psyr_1235 0.51 -0.0 1,393,887 + Psyr_1235 0.76 +0.7 1,394,105 + +0.8 1,394,105 + -2.0 1,394,106 - -2.8 1,394,121 + +0.5 1,394,122 - -0.2 1,394,200 + +0.6 1,394,200 + -0.8 1,394,200 + -0.5 1,394,247 + +0.3 1,394,247 + -1.3 1,394,343 + Psyr_1236 0.22 -2.3 1,394,343 + Psyr_1236 0.22 -3.2 1,394,356 + Psyr_1236 0.24 -4.0 1,394,639 + Psyr_1236 0.82 +1.0 1,394,700 + -0.4
Or see this region's nucleotide sequence