Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1039 and Psyr_1040 overlap by 4 nucleotides Psyr_1040 and Psyr_1041 are separated by 210 nucleotides Psyr_1041 and Psyr_1042 are separated by 136 nucleotides
Psyr_1039: Psyr_1039 - FecR protein, at 1,174,500 to 1,175,477
_1039
Psyr_1040: Psyr_1040 - RNA polymerase sigma factor, ECF subfamily, at 1,175,474 to 1,175,977
_1040
Psyr_1041: Psyr_1041 - transcriptional regulator, AraC family, at 1,176,188 to 1,177,063
_1041
Psyr_1042: Psyr_1042 - Protein of unknown function DUF6, at 1,177,200 to 1,178,102
_1042
Position (kb)
1176
1177
1178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1175.203 kb on - strand, within Psyr_1039 at 1175.478 kb on - strand at 1175.487 kb on + strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.488 kb on - strand at 1175.721 kb on + strand, within Psyr_1040 at 1175.721 kb on + strand, within Psyr_1040 at 1175.721 kb on + strand, within Psyr_1040 at 1175.722 kb on - strand, within Psyr_1040 at 1175.722 kb on - strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.724 kb on + strand, within Psyr_1040 at 1175.725 kb on - strand, within Psyr_1040 at 1175.819 kb on - strand, within Psyr_1040 at 1175.842 kb on + strand, within Psyr_1040 at 1175.931 kb on + strand at 1175.931 kb on + strand at 1175.931 kb on + strand at 1175.931 kb on + strand at 1176.125 kb on - strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.189 kb on + strand at 1176.190 kb on - strand at 1176.190 kb on - strand at 1176.210 kb on + strand at 1176.210 kb on + strand at 1176.211 kb on - strand at 1176.211 kb on - strand at 1176.361 kb on - strand, within Psyr_1041 at 1176.361 kb on - strand, within Psyr_1041 at 1176.492 kb on + strand, within Psyr_1041 at 1176.762 kb on + strand, within Psyr_1041 at 1176.762 kb on + strand, within Psyr_1041 at 1176.762 kb on + strand, within Psyr_1041 at 1176.763 kb on - strand, within Psyr_1041 at 1176.763 kb on - strand, within Psyr_1041 at 1176.763 kb on - strand, within Psyr_1041 at 1176.824 kb on + strand, within Psyr_1041 at 1176.825 kb on - strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.897 kb on + strand, within Psyr_1041 at 1176.898 kb on - strand, within Psyr_1041 at 1176.898 kb on - strand, within Psyr_1041 at 1176.898 kb on - strand, within Psyr_1041 at 1176.898 kb on - strand, within Psyr_1041 at 1176.898 kb on - strand, within Psyr_1041 at 1176.910 kb on + strand, within Psyr_1041 at 1176.911 kb on - strand, within Psyr_1041 at 1176.930 kb on + strand, within Psyr_1041 at 1176.931 kb on - strand, within Psyr_1041 at 1177.076 kb on - strand at 1177.084 kb on + strand at 1177.085 kb on - strand at 1177.089 kb on + strand at 1177.216 kb on - strand at 1177.343 kb on - strand, within Psyr_1042 at 1177.343 kb on - strand, within Psyr_1042 at 1177.440 kb on + strand, within Psyr_1042 at 1177.440 kb on + strand, within Psyr_1042 at 1177.440 kb on + strand, within Psyr_1042 at 1177.441 kb on - strand, within Psyr_1042 at 1177.441 kb on - strand, within Psyr_1042 at 1177.762 kb on - strand, within Psyr_1042 at 1177.762 kb on - strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.785 kb on + strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.786 kb on - strand, within Psyr_1042 at 1177.812 kb on - strand, within Psyr_1042 at 1177.834 kb on - strand, within Psyr_1042 at 1177.834 kb on - strand, within Psyr_1042 at 1177.834 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.838 kb on - strand, within Psyr_1042 at 1177.890 kb on + strand, within Psyr_1042 at 1177.891 kb on - strand, within Psyr_1042 at 1177.904 kb on - strand, within Psyr_1042 at 1177.933 kb on - strand, within Psyr_1042
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 1,175,203 - Psyr_1039 0.72 +1.1 1,175,478 - -1.9 1,175,487 + -0.8 1,175,488 - -1.6 1,175,488 - +0.0 1,175,488 - +0.0 1,175,488 - -2.7 1,175,488 - -1.7 1,175,721 + Psyr_1040 0.49 +0.5 1,175,721 + Psyr_1040 0.49 +0.2 1,175,721 + Psyr_1040 0.49 +0.1 1,175,722 - Psyr_1040 0.49 +0.9 1,175,722 - Psyr_1040 0.49 -0.1 1,175,724 + Psyr_1040 0.50 +0.7 1,175,724 + Psyr_1040 0.50 -0.5 1,175,724 + Psyr_1040 0.50 -0.1 1,175,725 - Psyr_1040 0.50 -2.9 1,175,819 - Psyr_1040 0.68 +0.4 1,175,842 + Psyr_1040 0.73 -0.5 1,175,931 + +0.9 1,175,931 + +0.4 1,175,931 + -0.6 1,175,931 + +0.2 1,176,125 - -0.9 1,176,189 + +0.3 1,176,189 + +1.4 1,176,189 + -3.0 1,176,189 + -1.5 1,176,189 + +2.9 1,176,190 - +2.1 1,176,190 - -1.5 1,176,210 + -1.9 1,176,210 + +1.6 1,176,211 - -0.9 1,176,211 - -2.0 1,176,361 - Psyr_1041 0.20 +1.3 1,176,361 - Psyr_1041 0.20 +0.3 1,176,492 + Psyr_1041 0.35 -1.4 1,176,762 + Psyr_1041 0.66 -2.2 1,176,762 + Psyr_1041 0.66 -2.7 1,176,762 + Psyr_1041 0.66 -0.6 1,176,763 - Psyr_1041 0.66 +0.1 1,176,763 - Psyr_1041 0.66 -0.2 1,176,763 - Psyr_1041 0.66 -1.2 1,176,824 + Psyr_1041 0.73 -0.5 1,176,825 - Psyr_1041 0.73 -0.9 1,176,897 + Psyr_1041 0.81 -0.8 1,176,897 + Psyr_1041 0.81 -3.4 1,176,897 + Psyr_1041 0.81 +0.1 1,176,897 + Psyr_1041 0.81 -1.0 1,176,897 + Psyr_1041 0.81 -0.2 1,176,897 + Psyr_1041 0.81 -1.0 1,176,897 + Psyr_1041 0.81 -0.6 1,176,897 + Psyr_1041 0.81 +0.4 1,176,897 + Psyr_1041 0.81 -0.9 1,176,897 + Psyr_1041 0.81 +1.5 1,176,897 + Psyr_1041 0.81 +2.8 1,176,897 + Psyr_1041 0.81 +0.4 1,176,898 - Psyr_1041 0.81 -0.5 1,176,898 - Psyr_1041 0.81 -0.5 1,176,898 - Psyr_1041 0.81 -1.8 1,176,898 - Psyr_1041 0.81 +0.6 1,176,898 - Psyr_1041 0.81 +1.3 1,176,910 + Psyr_1041 0.82 -1.9 1,176,911 - Psyr_1041 0.83 -1.1 1,176,930 + Psyr_1041 0.85 -1.3 1,176,931 - Psyr_1041 0.85 -0.1 1,177,076 - -0.7 1,177,084 + -1.1 1,177,085 - -0.4 1,177,089 + -0.6 1,177,216 - +1.4 1,177,343 - Psyr_1042 0.16 -0.1 1,177,343 - Psyr_1042 0.16 -1.5 1,177,440 + Psyr_1042 0.27 -2.9 1,177,440 + Psyr_1042 0.27 -0.2 1,177,440 + Psyr_1042 0.27 +0.4 1,177,441 - Psyr_1042 0.27 +0.8 1,177,441 - Psyr_1042 0.27 -2.2 1,177,762 - Psyr_1042 0.62 +1.2 1,177,762 - Psyr_1042 0.62 -0.2 1,177,785 + Psyr_1042 0.65 -0.8 1,177,785 + Psyr_1042 0.65 +0.3 1,177,785 + Psyr_1042 0.65 -0.1 1,177,785 + Psyr_1042 0.65 -0.6 1,177,785 + Psyr_1042 0.65 -1.9 1,177,785 + Psyr_1042 0.65 +2.4 1,177,785 + Psyr_1042 0.65 -0.2 1,177,785 + Psyr_1042 0.65 +0.3 1,177,785 + Psyr_1042 0.65 +0.4 1,177,785 + Psyr_1042 0.65 -0.0 1,177,785 + Psyr_1042 0.65 +0.3 1,177,785 + Psyr_1042 0.65 -1.3 1,177,785 + Psyr_1042 0.65 -0.5 1,177,785 + Psyr_1042 0.65 +1.4 1,177,786 - Psyr_1042 0.65 -0.3 1,177,786 - Psyr_1042 0.65 -0.4 1,177,786 - Psyr_1042 0.65 -0.2 1,177,786 - Psyr_1042 0.65 -2.1 1,177,786 - Psyr_1042 0.65 +0.7 1,177,786 - Psyr_1042 0.65 -0.8 1,177,786 - Psyr_1042 0.65 +0.9 1,177,786 - Psyr_1042 0.65 +0.4 1,177,786 - Psyr_1042 0.65 -3.1 1,177,786 - Psyr_1042 0.65 -1.9 1,177,786 - Psyr_1042 0.65 -0.1 1,177,786 - Psyr_1042 0.65 -0.2 1,177,786 - Psyr_1042 0.65 -2.1 1,177,786 - Psyr_1042 0.65 -1.6 1,177,786 - Psyr_1042 0.65 -0.7 1,177,812 - Psyr_1042 0.68 -0.9 1,177,834 - Psyr_1042 0.70 -0.7 1,177,834 - Psyr_1042 0.70 +2.1 1,177,834 - Psyr_1042 0.70 -0.4 1,177,838 - Psyr_1042 0.71 +0.5 1,177,838 - Psyr_1042 0.71 +0.2 1,177,838 - Psyr_1042 0.71 -0.4 1,177,838 - Psyr_1042 0.71 -0.9 1,177,838 - Psyr_1042 0.71 +0.1 1,177,838 - Psyr_1042 0.71 +0.1 1,177,838 - Psyr_1042 0.71 -2.6 1,177,890 + Psyr_1042 0.76 -1.9 1,177,891 - Psyr_1042 0.77 -0.3 1,177,904 - Psyr_1042 0.78 +0.1 1,177,933 - Psyr_1042 0.81 +3.7
Or see this region's nucleotide sequence