Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0547 and Psyr_0548 overlap by 10 nucleotides Psyr_0548 and Psyr_0549 are separated by 145 nucleotides Psyr_0549 and Psyr_0550 are separated by 88 nucleotides Psyr_0550 and Psyr_0551 are separated by 76 nucleotides
Psyr_0547: Psyr_0547 - Conserved hypothetical protein 52, at 619,676 to 620,323
_0547
Psyr_0548: Psyr_0548 - Protein of unknown function DUF1249, at 620,314 to 620,766
_0548
Psyr_0549: Psyr_0549 - Metallophosphoesterase, at 620,912 to 621,718
_0549
Psyr_0550: Psyr_0550 - Protein of unknown function UPF0227, at 621,807 to 622,424
_0550
Psyr_0551: Psyr_0551 - DNA topoisomerase IV subunit B, at 622,501 to 624,405
_0551
Position (kb) 620
621
622 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.313 kb on + strand at 620.314 kb on - strand at 620.314 kb on - strand at 620.327 kb on + strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.387 kb on - strand, within Psyr_0548 at 620.478 kb on - strand, within Psyr_0548 at 620.510 kb on - strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.584 kb on + strand, within Psyr_0548 at 620.585 kb on - strand, within Psyr_0548 at 620.647 kb on + strand, within Psyr_0548 at 620.647 kb on + strand, within Psyr_0548 at 620.647 kb on + strand, within Psyr_0548 at 620.647 kb on + strand, within Psyr_0548 at 620.648 kb on - strand, within Psyr_0548 at 620.648 kb on - strand, within Psyr_0548 at 620.648 kb on - strand, within Psyr_0548 at 621.117 kb on - strand, within Psyr_0549 at 621.448 kb on - strand, within Psyr_0549 at 621.486 kb on + strand, within Psyr_0549 at 621.733 kb on - strand at 621.768 kb on + strand at 621.773 kb on + strand at 621.773 kb on + strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.832 kb on + strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 622.030 kb on - strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.140 kb on + strand, within Psyr_0550 at 622.287 kb on + strand, within Psyr_0550 at 622.422 kb on + strand at 622.422 kb on + strand at 622.422 kb on + strand at 622.422 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 620,081 + Psyr_0547 0.62 -0.5 620,081 + Psyr_0547 0.62 -1.4 620,081 + Psyr_0547 0.62 +2.7 620,081 + Psyr_0547 0.62 -2.9 620,081 + Psyr_0547 0.62 +0.6 620,081 + Psyr_0547 0.62 -1.0 620,081 + Psyr_0547 0.62 -2.1 620,082 - Psyr_0547 0.63 -3.8 620,082 - Psyr_0547 0.63 -0.9 620,082 - Psyr_0547 0.63 -0.3 620,082 - Psyr_0547 0.63 -0.3 620,082 - Psyr_0547 0.63 -4.1 620,082 - Psyr_0547 0.63 +0.3 620,082 - Psyr_0547 0.63 -0.7 620,082 - Psyr_0547 0.63 -0.6 620,082 - Psyr_0547 0.63 -1.0 620,313 + +0.6 620,314 - +0.2 620,314 - +1.2 620,327 + -2.2 620,328 - -0.9 620,328 - -0.5 620,328 - -0.4 620,387 - Psyr_0548 0.16 -2.7 620,478 - Psyr_0548 0.36 -1.7 620,510 - Psyr_0548 0.43 +1.1 620,584 + Psyr_0548 0.60 -0.7 620,584 + Psyr_0548 0.60 -0.8 620,584 + Psyr_0548 0.60 -2.3 620,584 + Psyr_0548 0.60 -0.6 620,584 + Psyr_0548 0.60 +1.8 620,584 + Psyr_0548 0.60 -0.1 620,585 - Psyr_0548 0.60 +0.6 620,647 + Psyr_0548 0.74 -2.3 620,647 + Psyr_0548 0.74 -1.3 620,647 + Psyr_0548 0.74 -1.7 620,647 + Psyr_0548 0.74 -0.3 620,648 - Psyr_0548 0.74 -1.0 620,648 - Psyr_0548 0.74 -0.3 620,648 - Psyr_0548 0.74 -1.5 621,117 - Psyr_0549 0.25 +0.7 621,448 - Psyr_0549 0.66 -2.1 621,486 + Psyr_0549 0.71 -2.8 621,733 - -0.6 621,768 + +0.8 621,773 + +1.4 621,773 + +0.5 621,774 - -0.9 621,774 - -3.1 621,774 - -1.6 621,774 - -2.1 621,774 - -2.2 621,832 + -0.0 621,833 - +0.1 621,833 - -0.4 621,833 - -2.7 621,833 - -0.9 621,834 + -2.6 621,834 + -2.7 621,834 + -3.6 621,834 + -1.5 621,834 + +1.1 621,834 + -2.0 621,834 + +1.1 621,834 + -1.9 621,834 + -4.3 621,834 + -1.8 621,834 + -2.1 621,834 + -2.2 621,834 + -1.4 621,834 + -1.7 621,834 + -1.6 621,834 + -2.6 621,834 + -0.3 621,834 + -2.2 621,834 + +1.1 621,835 - -0.5 621,835 - -2.3 621,835 - -1.8 621,835 - +1.1 621,835 - -2.1 621,835 - -1.2 621,835 - -1.4 621,835 - -1.2 621,835 - -2.1 621,835 - -4.5 621,835 - -2.7 621,835 - -2.4 621,835 - -0.9 621,835 - +0.1 621,835 - -2.3 621,835 - -1.0 621,835 - -3.0 621,835 - -3.3 621,835 - +0.3 621,835 - -3.0 621,835 - -3.4 621,835 - -1.0 621,835 - -1.5 621,835 - -1.2 621,835 - -0.9 621,835 - -1.9 621,835 - +0.1 621,835 - -1.2 621,835 - -2.3 621,835 - -2.3 622,030 - Psyr_0550 0.36 -0.4 622,032 + Psyr_0550 0.36 -1.7 622,032 + Psyr_0550 0.36 -1.0 622,032 + Psyr_0550 0.36 -3.0 622,140 + Psyr_0550 0.54 -1.9 622,287 + Psyr_0550 0.78 -2.0 622,422 + -0.4 622,422 + +0.8 622,422 + +0.7 622,422 + -2.4
Or see this region's nucleotide sequence