Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0537 and Psyr_0538 are separated by 108 nucleotides Psyr_0538 and Psyr_0539 are separated by 36 nucleotides Psyr_0539 and Psyr_0540 overlap by 4 nucleotides
Psyr_0537: Psyr_0537 - D-alpha,beta-D-heptose 7-phosphate 1-kinase / D-beta-D-heptose 1-phosphate adenylyltransferase, at 606,836 to 608,260
_0537
Psyr_0538: Psyr_0538 - conserved hypothetical protein, at 608,369 to 609,325
_0538
Psyr_0539: Psyr_0539 - Aldo/keto reductase, at 609,362 to 610,177
_0539
Psyr_0540: Psyr_0540 - oxidoreductase, FAD-binding protein, at 610,174 to 611,349
_0540
Position (kb)
608
609
610 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 608.265 kb on + strand at 608.265 kb on + strand at 608.266 kb on - strand at 608.276 kb on + strand at 608.276 kb on + strand at 608.276 kb on + strand at 608.276 kb on + strand at 608.277 kb on - strand at 608.277 kb on - strand at 608.277 kb on - strand at 608.290 kb on + strand at 608.290 kb on + strand at 608.290 kb on + strand at 608.291 kb on - strand at 608.291 kb on - strand at 608.291 kb on - strand at 608.291 kb on - strand at 608.291 kb on - strand at 608.297 kb on + strand at 608.297 kb on + strand at 608.297 kb on + strand at 608.297 kb on + strand at 608.298 kb on - strand at 608.298 kb on - strand at 608.298 kb on - strand at 608.298 kb on - strand at 608.298 kb on - strand at 608.298 kb on - strand at 608.541 kb on + strand, within Psyr_0538 at 608.541 kb on + strand, within Psyr_0538 at 608.542 kb on - strand, within Psyr_0538 at 608.589 kb on + strand, within Psyr_0538 at 608.590 kb on - strand, within Psyr_0538 at 608.590 kb on - strand, within Psyr_0538 at 608.590 kb on - strand, within Psyr_0538 at 608.590 kb on - strand, within Psyr_0538 at 608.631 kb on + strand, within Psyr_0538 at 608.632 kb on - strand, within Psyr_0538 at 608.632 kb on - strand, within Psyr_0538 at 608.749 kb on - strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.784 kb on + strand, within Psyr_0538 at 608.785 kb on - strand, within Psyr_0538 at 608.785 kb on - strand, within Psyr_0538 at 608.785 kb on - strand, within Psyr_0538 at 609.027 kb on + strand, within Psyr_0538 at 609.028 kb on - strand, within Psyr_0538 at 609.241 kb on - strand at 609.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 608,265 + +0.4 608,265 + -0.8 608,266 - -0.6 608,276 + +0.1 608,276 + +1.1 608,276 + -0.3 608,276 + -0.4 608,277 - -2.0 608,277 - -1.1 608,277 - -0.4 608,290 + +1.6 608,290 + +0.5 608,290 + +0.6 608,291 - +1.1 608,291 - +0.1 608,291 - +0.5 608,291 - +1.9 608,291 - -1.1 608,297 + +0.4 608,297 + -1.0 608,297 + +0.6 608,297 + -2.4 608,298 - -1.9 608,298 - +1.6 608,298 - +2.9 608,298 - -0.5 608,298 - -1.8 608,298 - -0.1 608,541 + Psyr_0538 0.18 -2.4 608,541 + Psyr_0538 0.18 -2.2 608,542 - Psyr_0538 0.18 -1.9 608,589 + Psyr_0538 0.23 +0.7 608,590 - Psyr_0538 0.23 -1.4 608,590 - Psyr_0538 0.23 -0.2 608,590 - Psyr_0538 0.23 -0.4 608,590 - Psyr_0538 0.23 -3.4 608,631 + Psyr_0538 0.27 -2.2 608,632 - Psyr_0538 0.27 +0.6 608,632 - Psyr_0538 0.27 -0.9 608,749 - Psyr_0538 0.40 -1.2 608,784 + Psyr_0538 0.43 -2.3 608,784 + Psyr_0538 0.43 -1.4 608,784 + Psyr_0538 0.43 -2.6 608,784 + Psyr_0538 0.43 -1.6 608,784 + Psyr_0538 0.43 -2.6 608,784 + Psyr_0538 0.43 +2.0 608,784 + Psyr_0538 0.43 -2.9 608,785 - Psyr_0538 0.43 -2.4 608,785 - Psyr_0538 0.43 -1.2 608,785 - Psyr_0538 0.43 -1.4 609,027 + Psyr_0538 0.69 +0.0 609,028 - Psyr_0538 0.69 -1.8 609,241 - +1.8 609,241 - +1.1
Or see this region's nucleotide sequence