Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0224 and Psyr_0225 are separated by 11 nucleotides Psyr_0225 and Psyr_0226 are separated by 81 nucleotides Psyr_0226 and Psyr_0227 are separated by 233 nucleotides Psyr_0227 and Psyr_0228 are separated by 242 nucleotides
Psyr_0224: Psyr_0224 - LSU ribosomal protein L28P, at 239,211 to 239,444
_0224
Psyr_0225: Psyr_0225 - LSU ribosomal protein L33P, at 239,456 to 239,611
_0225
Psyr_0226: Psyr_0226 - Protein of unknown function DUF861, at 239,693 to 240,061
_0226
Psyr_0227: Psyr_0227 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase, at 240,295 to 241,794
_0227
Psyr_0228: Psyr_0228 - Major facilitator superfamily, at 242,037 to 243,185
_0228
Position (kb) 240
241
242 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 239.690 kb on + strand at 239.691 kb on - strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.698 kb on + strand at 239.698 kb on + strand at 239.699 kb on - strand at 239.699 kb on - strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.758 kb on + strand, within Psyr_0226 at 239.758 kb on + strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.862 kb on + strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.863 kb on - strand, within Psyr_0226 at 239.877 kb on + strand, within Psyr_0226 at 239.877 kb on + strand, within Psyr_0226 at 239.878 kb on - strand, within Psyr_0226 at 239.882 kb on + strand, within Psyr_0226 at 239.882 kb on + strand, within Psyr_0226 at 239.883 kb on - strand, within Psyr_0226 at 239.946 kb on + strand, within Psyr_0226 at 239.946 kb on + strand, within Psyr_0226 at 239.947 kb on - strand, within Psyr_0226 at 239.947 kb on - strand, within Psyr_0226 at 239.955 kb on + strand, within Psyr_0226 at 239.956 kb on - strand, within Psyr_0226 at 239.956 kb on - strand, within Psyr_0226 at 240.007 kb on - strand, within Psyr_0226 at 240.146 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.157 kb on + strand at 240.158 kb on - strand at 240.158 kb on - strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.179 kb on + strand at 240.180 kb on - strand at 240.180 kb on - strand at 240.180 kb on - strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.188 kb on + strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.189 kb on - strand at 240.190 kb on + strand at 240.215 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.382 kb on + strand at 240.383 kb on - strand at 240.383 kb on - strand at 240.663 kb on - strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.715 kb on + strand, within Psyr_0227 at 240.716 kb on - strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.956 kb on + strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 240.957 kb on - strand, within Psyr_0227 at 241.002 kb on + strand, within Psyr_0227 at 241.003 kb on - strand, within Psyr_0227 at 241.063 kb on + strand, within Psyr_0227 at 241.063 kb on + strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.064 kb on - strand, within Psyr_0227 at 241.353 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.366 kb on + strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.367 kb on - strand, within Psyr_0227 at 241.368 kb on + strand, within Psyr_0227 at 241.368 kb on + strand, within Psyr_0227 at 241.383 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.444 kb on + strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.445 kb on - strand, within Psyr_0227 at 241.599 kb on - strand, within Psyr_0227 at 241.659 kb on - strand at 241.659 kb on - strand at 241.672 kb on + strand at 241.759 kb on + strand at 241.759 kb on + strand at 241.759 kb on + strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.760 kb on - strand at 241.792 kb on + strand at 241.792 kb on + strand at 241.792 kb on + strand at 241.793 kb on - strand at 241.793 kb on - strand at 241.793 kb on - strand at 241.991 kb on + strand at 241.991 kb on + strand at 241.992 kb on - strand at 241.992 kb on - strand at 242.049 kb on - strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.052 kb on + strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.053 kb on - strand at 242.307 kb on + strand, within Psyr_0228 at 242.310 kb on + strand, within Psyr_0228 at 242.433 kb on + strand, within Psyr_0228 at 242.445 kb on + strand, within Psyr_0228 at 242.445 kb on + strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.446 kb on - strand, within Psyr_0228 at 242.703 kb on + strand, within Psyr_0228 at 242.704 kb on - strand, within Psyr_0228 at 242.711 kb on - strand, within Psyr_0228 at 242.711 kb on - strand, within Psyr_0228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 239,690 + -0.2 239,691 - +1.7 239,694 + -1.0 239,694 + -0.6 239,694 + +0.6 239,694 + +0.3 239,694 + +2.5 239,694 + -0.1 239,695 - +1.0 239,695 - -0.2 239,695 - +0.8 239,695 - -1.9 239,695 - -0.4 239,695 - -0.9 239,698 + +0.1 239,698 + -0.8 239,699 - +0.3 239,699 - +0.6 239,724 + -1.4 239,724 + +1.1 239,724 + -0.7 239,725 - -3.6 239,725 - -0.2 239,725 - +0.9 239,725 - +0.5 239,725 - +0.5 239,725 - -0.1 239,758 + Psyr_0226 0.18 -0.2 239,758 + Psyr_0226 0.18 -0.0 239,759 - Psyr_0226 0.18 -0.6 239,759 - Psyr_0226 0.18 +0.2 239,759 - Psyr_0226 0.18 +1.3 239,862 + Psyr_0226 0.46 +0.7 239,862 + Psyr_0226 0.46 -1.3 239,862 + Psyr_0226 0.46 -0.1 239,863 - Psyr_0226 0.46 +1.4 239,863 - Psyr_0226 0.46 +1.0 239,863 - Psyr_0226 0.46 +0.2 239,863 - Psyr_0226 0.46 -1.0 239,877 + Psyr_0226 0.50 +0.9 239,877 + Psyr_0226 0.50 -1.8 239,878 - Psyr_0226 0.50 +3.0 239,882 + Psyr_0226 0.51 +0.8 239,882 + Psyr_0226 0.51 -1.4 239,883 - Psyr_0226 0.51 -0.3 239,946 + Psyr_0226 0.69 -2.5 239,946 + Psyr_0226 0.69 +0.4 239,947 - Psyr_0226 0.69 +2.9 239,947 - Psyr_0226 0.69 +0.1 239,955 + Psyr_0226 0.71 -0.7 239,956 - Psyr_0226 0.71 +0.1 239,956 - Psyr_0226 0.71 -1.7 240,007 - Psyr_0226 0.85 -0.1 240,146 + -2.7 240,157 + +0.6 240,157 + +2.0 240,157 + -0.2 240,157 + +0.1 240,157 + -1.4 240,157 + +0.6 240,158 - +0.1 240,158 - +1.2 240,179 + +0.3 240,179 + +0.3 240,179 + +0.0 240,179 + +0.1 240,180 - -0.2 240,180 - -1.6 240,180 - +0.3 240,188 + +1.3 240,188 + +1.2 240,188 + -0.5 240,188 + +1.3 240,188 + +1.1 240,189 - +1.2 240,189 - +0.9 240,189 - +1.2 240,189 - -0.8 240,190 + +1.4 240,215 + -3.5 240,382 + -2.1 240,382 + +0.2 240,382 + +0.8 240,382 + +1.2 240,382 + +0.9 240,383 - -4.3 240,383 - -1.4 240,663 - Psyr_0227 0.25 -1.4 240,715 + Psyr_0227 0.28 +1.1 240,715 + Psyr_0227 0.28 -1.9 240,715 + Psyr_0227 0.28 +0.4 240,715 + Psyr_0227 0.28 -0.7 240,715 + Psyr_0227 0.28 +1.6 240,715 + Psyr_0227 0.28 -3.4 240,715 + Psyr_0227 0.28 -1.9 240,716 - Psyr_0227 0.28 -0.7 240,956 + Psyr_0227 0.44 +1.4 240,956 + Psyr_0227 0.44 +3.5 240,956 + Psyr_0227 0.44 +0.7 240,956 + Psyr_0227 0.44 +1.6 240,956 + Psyr_0227 0.44 -1.0 240,957 - Psyr_0227 0.44 -0.5 240,957 - Psyr_0227 0.44 +0.6 240,957 - Psyr_0227 0.44 -3.6 240,957 - Psyr_0227 0.44 +1.0 241,002 + Psyr_0227 0.47 +0.6 241,003 - Psyr_0227 0.47 -1.0 241,063 + Psyr_0227 0.51 -0.5 241,063 + Psyr_0227 0.51 +0.1 241,064 - Psyr_0227 0.51 -0.2 241,064 - Psyr_0227 0.51 +0.3 241,064 - Psyr_0227 0.51 -1.4 241,064 - Psyr_0227 0.51 -1.9 241,353 + Psyr_0227 0.71 -0.2 241,366 + Psyr_0227 0.71 +0.8 241,366 + Psyr_0227 0.71 -0.1 241,366 + Psyr_0227 0.71 +0.6 241,366 + Psyr_0227 0.71 +0.1 241,366 + Psyr_0227 0.71 -0.5 241,366 + Psyr_0227 0.71 -0.2 241,366 + Psyr_0227 0.71 +1.1 241,366 + Psyr_0227 0.71 -2.2 241,366 + Psyr_0227 0.71 -0.6 241,366 + Psyr_0227 0.71 -1.9 241,366 + Psyr_0227 0.71 +1.0 241,367 - Psyr_0227 0.71 -2.4 241,367 - Psyr_0227 0.71 -0.2 241,367 - Psyr_0227 0.71 -1.6 241,367 - Psyr_0227 0.71 -2.4 241,367 - Psyr_0227 0.71 -0.6 241,367 - Psyr_0227 0.71 +0.2 241,367 - Psyr_0227 0.71 +0.5 241,367 - Psyr_0227 0.71 +0.1 241,368 + Psyr_0227 0.72 +0.1 241,368 + Psyr_0227 0.72 +1.5 241,383 + Psyr_0227 0.73 +0.2 241,444 + Psyr_0227 0.77 +1.8 241,444 + Psyr_0227 0.77 -3.2 241,444 + Psyr_0227 0.77 +1.3 241,444 + Psyr_0227 0.77 -0.8 241,444 + Psyr_0227 0.77 -1.4 241,444 + Psyr_0227 0.77 -2.0 241,444 + Psyr_0227 0.77 -0.2 241,444 + Psyr_0227 0.77 -0.9 241,444 + Psyr_0227 0.77 +0.1 241,445 - Psyr_0227 0.77 -1.2 241,445 - Psyr_0227 0.77 -0.1 241,445 - Psyr_0227 0.77 +2.8 241,445 - Psyr_0227 0.77 -1.3 241,445 - Psyr_0227 0.77 -0.1 241,445 - Psyr_0227 0.77 -1.5 241,445 - Psyr_0227 0.77 -0.3 241,599 - Psyr_0227 0.87 +0.7 241,659 - -1.1 241,659 - -1.4 241,672 + -1.6 241,759 + +0.4 241,759 + -1.8 241,759 + +0.0 241,760 - -1.3 241,760 - -0.4 241,760 - -1.7 241,760 - -0.8 241,760 - -2.0 241,792 + -1.2 241,792 + -0.0 241,792 + -0.8 241,793 - -1.2 241,793 - +0.4 241,793 - +0.0 241,991 + -2.9 241,991 + -2.2 241,992 - +2.0 241,992 - +0.8 242,049 - +0.5 242,052 + -0.1 242,052 + +2.2 242,052 + +1.9 242,052 + -0.3 242,052 + +1.7 242,052 + +0.3 242,053 - +0.8 242,053 - +0.6 242,053 - +0.8 242,053 - -0.0 242,053 - -1.4 242,307 + Psyr_0228 0.23 -0.6 242,310 + Psyr_0228 0.24 +1.2 242,433 + Psyr_0228 0.34 -1.0 242,445 + Psyr_0228 0.36 -3.5 242,445 + Psyr_0228 0.36 +1.0 242,446 - Psyr_0228 0.36 -1.5 242,446 - Psyr_0228 0.36 -2.6 242,446 - Psyr_0228 0.36 -0.0 242,446 - Psyr_0228 0.36 +0.3 242,703 + Psyr_0228 0.58 -0.4 242,704 - Psyr_0228 0.58 -3.2 242,711 - Psyr_0228 0.59 -0.8 242,711 - Psyr_0228 0.59 -2.3
Or see this region's nucleotide sequence