Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4337 and BT4338 are separated by 52 nucleotides BT4338 and BT4339 are separated by 117 nucleotides BT4339 and BT4340 are separated by 182 nucleotides BT4340 and BT4341 are separated by 428 nucleotides
BT4337: BT4337 - beta-hexosaminidase precursor (NCBI ptt file), at 5,711,295 to 5,713,829
BT4337
BT4338: BT4338 - CRP family transcriptional regulator with cAMP-binding domain (NCBI ptt file), at 5,713,882 to 5,714,583
BT4338
BT4339: BT4339 - glutamine synthetase (NCBI ptt file), at 5,714,701 to 5,716,890
BT4339
BT4340: BT4340 - hypothetical protein (NCBI ptt file), at 5,717,073 to 5,717,273
BT4340
BT4341: BT4341 - hypothetical protein (NCBI ptt file), at 5,717,702 to 5,719,861
BT4341
Position (kb)
5714
5715
5716
5717 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 5713.751 kb on + strand at 5713.752 kb on - strand at 5713.770 kb on + strand at 5713.785 kb on + strand at 5713.785 kb on + strand at 5713.799 kb on - strand at 5713.800 kb on + strand at 5713.800 kb on + strand at 5713.870 kb on - strand at 5713.870 kb on - strand at 5713.870 kb on - strand at 5713.870 kb on - strand at 5713.881 kb on - strand at 5713.881 kb on - strand at 5714.848 kb on - strand at 5715.573 kb on + strand, within BT4339 at 5715.864 kb on + strand, within BT4339 at 5715.914 kb on - strand, within BT4339 at 5716.213 kb on - strand, within BT4339 at 5716.215 kb on - strand, within BT4339 at 5716.322 kb on - strand, within BT4339 at 5716.418 kb on - strand, within BT4339 at 5716.473 kb on + strand, within BT4339 at 5716.631 kb on + strand, within BT4339 at 5716.761 kb on - strand at 5717.064 kb on + strand at 5717.067 kb on - strand at 5717.154 kb on + strand, within BT4340 at 5717.169 kb on - strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.171 kb on - strand, within BT4340 at 5717.211 kb on - strand, within BT4340 at 5717.241 kb on + strand, within BT4340 at 5717.380 kb on + strand at 5717.381 kb on - strand at 5717.425 kb on - strand at 5717.458 kb on + strand at 5717.461 kb on + strand at 5717.462 kb on - strand at 5717.527 kb on + strand at 5717.535 kb on - strand at 5717.668 kb on - strand at 5717.683 kb on + strand at 5717.688 kb on + strand at 5717.702 kb on - strand at 5717.702 kb on - strand at 5717.753 kb on - strand at 5717.761 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.858 kb on + strand at 5717.860 kb on + strand at 5717.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 5,713,751 + +1.7 5,713,752 - -1.9 5,713,770 + -2.5 5,713,785 + -1.3 5,713,785 + +1.1 5,713,799 - -1.9 5,713,800 + +0.1 5,713,800 + -0.9 5,713,870 - -1.1 5,713,870 - +2.1 5,713,870 - -1.4 5,713,870 - -2.5 5,713,881 - -1.5 5,713,881 - -1.9 5,714,848 - +0.1 5,715,573 + BT4339 0.40 +6.5 5,715,864 + BT4339 0.53 +0.1 5,715,914 - BT4339 0.55 -1.9 5,716,213 - BT4339 0.69 +2.1 5,716,215 - BT4339 0.69 -0.9 5,716,322 - BT4339 0.74 -0.9 5,716,418 - BT4339 0.78 +1.4 5,716,473 + BT4339 0.81 +0.8 5,716,631 + BT4339 0.88 +0.7 5,716,761 - -0.5 5,717,064 + -0.9 5,717,067 - -1.9 5,717,154 + BT4340 0.40 -2.1 5,717,169 - BT4340 0.48 -2.1 5,717,170 + BT4340 0.48 +0.1 5,717,170 + BT4340 0.48 +0.1 5,717,171 - BT4340 0.49 -1.7 5,717,211 - BT4340 0.69 -2.2 5,717,241 + BT4340 0.84 -1.2 5,717,380 + -1.9 5,717,381 - +1.1 5,717,425 - -3.7 5,717,458 + +1.7 5,717,461 + -1.5 5,717,462 - -2.3 5,717,527 + -1.5 5,717,535 - -1.5 5,717,668 - -1.6 5,717,683 + +0.3 5,717,688 + -1.9 5,717,702 - -2.6 5,717,702 - -2.7 5,717,753 - -0.7 5,717,761 + -0.7 5,717,856 + -2.9 5,717,856 + -2.7 5,717,856 + +1.1 5,717,858 + +0.6 5,717,860 + -1.7 5,717,863 - -2.2
Or see this region's nucleotide sequence