Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4310 and BT4311 are separated by 144 nucleotides BT4311 and BT4312 are separated by 133 nucleotides
BT4310: BT4310 - NADPH-dependent glutamate synthase small chain (NCBI ptt file), at 5,679,083 to 5,681,374
BT4310
BT4311: BT4311 - glucose/galactose transporter (NCBI ptt file), at 5,681,519 to 5,682,679
BT4311
BT4312: BT4312 - seryl-tRNA synthetase (NCBI ptt file), at 5,682,813 to 5,684,087
BT4312
Position (kb)
5681
5682
5683 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5680.535 kb on + strand, within BT4310 at 5680.610 kb on - strand, within BT4310 at 5680.610 kb on - strand, within BT4310 at 5680.674 kb on - strand, within BT4310 at 5680.793 kb on + strand, within BT4310 at 5680.913 kb on - strand, within BT4310 at 5680.913 kb on - strand, within BT4310 at 5680.972 kb on + strand, within BT4310 at 5681.029 kb on + strand, within BT4310 at 5681.051 kb on + strand, within BT4310 at 5681.098 kb on + strand, within BT4310 at 5681.117 kb on + strand, within BT4310 at 5681.118 kb on - strand, within BT4310 at 5681.134 kb on - strand, within BT4310 at 5681.243 kb on - strand at 5681.306 kb on + strand at 5681.306 kb on + strand at 5681.511 kb on - strand at 5681.592 kb on + strand at 5681.634 kb on - strand at 5681.645 kb on - strand, within BT4311 at 5681.745 kb on - strand, within BT4311 at 5681.779 kb on - strand, within BT4311 at 5681.779 kb on - strand, within BT4311 at 5681.786 kb on + strand, within BT4311 at 5681.786 kb on + strand, within BT4311 at 5681.787 kb on - strand, within BT4311 at 5681.789 kb on - strand, within BT4311 at 5681.807 kb on + strand, within BT4311 at 5681.810 kb on + strand at 5681.811 kb on - strand, within BT4311 at 5681.870 kb on - strand, within BT4311 at 5681.872 kb on - strand, within BT4311 at 5681.901 kb on + strand, within BT4311 at 5681.938 kb on - strand, within BT4311 at 5681.982 kb on - strand, within BT4311 at 5681.987 kb on - strand, within BT4311 at 5681.996 kb on + strand, within BT4311 at 5682.005 kb on + strand, within BT4311 at 5682.012 kb on - strand, within BT4311 at 5682.012 kb on - strand, within BT4311 at 5682.015 kb on + strand, within BT4311 at 5682.016 kb on - strand, within BT4311 at 5682.018 kb on - strand, within BT4311 at 5682.038 kb on + strand, within BT4311 at 5682.039 kb on - strand, within BT4311 at 5682.041 kb on + strand, within BT4311 at 5682.042 kb on - strand, within BT4311 at 5682.044 kb on + strand, within BT4311 at 5682.084 kb on + strand, within BT4311 at 5682.157 kb on - strand, within BT4311 at 5682.166 kb on + strand, within BT4311 at 5682.199 kb on - strand, within BT4311 at 5682.199 kb on - strand, within BT4311 at 5682.221 kb on + strand, within BT4311 at 5682.230 kb on - strand, within BT4311 at 5682.299 kb on + strand, within BT4311 at 5682.299 kb on + strand, within BT4311 at 5682.300 kb on - strand, within BT4311 at 5682.358 kb on - strand, within BT4311 at 5682.438 kb on + strand, within BT4311 at 5682.452 kb on + strand, within BT4311 at 5682.453 kb on + strand, within BT4311 at 5682.481 kb on + strand, within BT4311 at 5682.481 kb on + strand, within BT4311 at 5682.482 kb on - strand, within BT4311 at 5682.530 kb on - strand, within BT4311 at 5682.636 kb on - strand at 5682.638 kb on + strand at 5682.650 kb on - strand at 5682.755 kb on + strand at 5682.784 kb on - strand at 5682.803 kb on - strand at 5682.815 kb on - strand at 5682.820 kb on - strand at 5682.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 5,680,535 + BT4310 0.63 -2.7 5,680,610 - BT4310 0.67 -1.7 5,680,610 - BT4310 0.67 -2.0 5,680,674 - BT4310 0.69 -2.9 5,680,793 + BT4310 0.75 -1.1 5,680,913 - BT4310 0.80 -2.3 5,680,913 - BT4310 0.80 -1.3 5,680,972 + BT4310 0.82 -2.4 5,681,029 + BT4310 0.85 -3.2 5,681,051 + BT4310 0.86 -0.7 5,681,098 + BT4310 0.88 -2.7 5,681,117 + BT4310 0.89 -1.9 5,681,118 - BT4310 0.89 -4.4 5,681,134 - BT4310 0.89 -2.2 5,681,243 - -2.4 5,681,306 + -1.5 5,681,306 + -1.8 5,681,511 - +0.7 5,681,592 + -1.5 5,681,634 - -1.9 5,681,645 - BT4311 0.11 -1.9 5,681,745 - BT4311 0.19 +0.1 5,681,779 - BT4311 0.22 -0.9 5,681,779 - BT4311 0.22 -0.5 5,681,786 + BT4311 0.23 -0.9 5,681,786 + BT4311 0.23 -2.4 5,681,787 - BT4311 0.23 +0.1 5,681,789 - BT4311 0.23 -1.8 5,681,807 + BT4311 0.25 +0.1 5,681,810 + -0.9 5,681,811 - BT4311 0.25 -2.1 5,681,870 - BT4311 0.30 +0.1 5,681,872 - BT4311 0.30 -0.9 5,681,901 + BT4311 0.33 -2.2 5,681,938 - BT4311 0.36 +0.7 5,681,982 - BT4311 0.40 +0.1 5,681,987 - BT4311 0.40 -2.3 5,681,996 + BT4311 0.41 -3.1 5,682,005 + BT4311 0.42 -1.5 5,682,012 - BT4311 0.42 -0.7 5,682,012 - BT4311 0.42 +1.3 5,682,015 + BT4311 0.43 +1.7 5,682,016 - BT4311 0.43 +0.8 5,682,018 - BT4311 0.43 +4.0 5,682,038 + BT4311 0.45 -2.7 5,682,039 - BT4311 0.45 -1.5 5,682,041 + BT4311 0.45 -0.2 5,682,042 - BT4311 0.45 +0.1 5,682,044 + BT4311 0.45 +3.1 5,682,084 + BT4311 0.49 -1.1 5,682,157 - BT4311 0.55 +0.5 5,682,166 + BT4311 0.56 -3.0 5,682,199 - BT4311 0.59 -3.1 5,682,199 - BT4311 0.59 -0.9 5,682,221 + BT4311 0.60 -1.7 5,682,230 - BT4311 0.61 -0.4 5,682,299 + BT4311 0.67 -4.2 5,682,299 + BT4311 0.67 -1.5 5,682,300 - BT4311 0.67 +1.1 5,682,358 - BT4311 0.72 -3.1 5,682,438 + BT4311 0.79 +0.3 5,682,452 + BT4311 0.80 +0.1 5,682,453 + BT4311 0.80 -1.5 5,682,481 + BT4311 0.83 +0.1 5,682,481 + BT4311 0.83 -1.2 5,682,482 - BT4311 0.83 -0.9 5,682,530 - BT4311 0.87 +0.1 5,682,636 - -1.5 5,682,638 + -1.7 5,682,650 - +1.9 5,682,755 + -1.9 5,682,784 - -1.8 5,682,803 - +0.1 5,682,815 - -2.4 5,682,820 - -0.6 5,682,820 - -2.6
Or see this region's nucleotide sequence