Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4248

Experiment: BHIS with Cefoxitin 0.025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4247 and BT4248 are separated by 50 nucleotidesBT4248 and BT4249 are separated by 266 nucleotidesBT4249 and BT4250 are separated by 129 nucleotides BT4247: BT4247 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,596,039 to 5,599,392 BT4247 BT4248: BT4248 - putative anti-sigma factor (NCBI ptt file), at 5,599,443 to 5,600,096 BT4248 BT4249: BT4249 - hypothetical protein (NCBI ptt file), at 5,600,363 to 5,600,596 BT4249 BT4250: BT4250 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,600,726 to 5,601,271 BT4250 Position (kb) 5599 5600 5601Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5598.443 kb on - strand, within BT4247at 5598.467 kb on + strand, within BT4247at 5598.467 kb on + strand, within BT4247at 5598.492 kb on + strand, within BT4247at 5598.534 kb on + strand, within BT4247at 5598.535 kb on - strand, within BT4247at 5598.551 kb on - strand, within BT4247at 5598.551 kb on - strand, within BT4247at 5598.556 kb on + strand, within BT4247at 5598.556 kb on + strand, within BT4247at 5598.557 kb on - strand, within BT4247at 5598.735 kb on + strand, within BT4247at 5598.744 kb on + strand, within BT4247at 5598.744 kb on + strand, within BT4247at 5598.745 kb on - strand, within BT4247at 5598.873 kb on + strand, within BT4247at 5598.885 kb on - strand, within BT4247at 5598.987 kb on + strand, within BT4247at 5598.987 kb on + strand, within BT4247at 5599.022 kb on - strand, within BT4247at 5599.024 kb on - strandat 5599.112 kb on + strandat 5599.113 kb on - strandat 5599.117 kb on - strandat 5599.159 kb on - strandat 5599.214 kb on + strandat 5599.215 kb on - strandat 5599.215 kb on - strandat 5599.245 kb on + strandat 5599.263 kb on + strandat 5599.263 kb on + strandat 5599.267 kb on - strandat 5599.292 kb on - strandat 5599.292 kb on - strandat 5599.292 kb on - strandat 5599.466 kb on + strandat 5599.509 kb on - strand, within BT4248at 5599.509 kb on - strand, within BT4248at 5599.512 kb on + strand, within BT4248at 5599.698 kb on + strand, within BT4248at 5599.723 kb on + strand, within BT4248at 5599.723 kb on + strand, within BT4248at 5599.724 kb on - strand, within BT4248at 5599.728 kb on + strand, within BT4248at 5599.729 kb on - strand, within BT4248at 5599.766 kb on + strand, within BT4248at 5599.774 kb on - strand, within BT4248at 5599.866 kb on - strand, within BT4248at 5599.877 kb on + strand, within BT4248at 5599.910 kb on + strand, within BT4248at 5599.912 kb on + strand, within BT4248at 5599.944 kb on + strand, within BT4248at 5599.989 kb on - strand, within BT4248at 5600.013 kb on - strand, within BT4248at 5600.013 kb on - strand, within BT4248at 5600.109 kb on + strandat 5600.170 kb on - strandat 5600.236 kb on - strandat 5600.322 kb on + strandat 5600.338 kb on + strandat 5600.355 kb on - strandat 5600.359 kb on + strandat 5600.360 kb on - strandat 5600.392 kb on + strand, within BT4249at 5600.438 kb on + strand, within BT4249at 5600.476 kb on - strand, within BT4249at 5600.608 kb on - strandat 5600.652 kb on - strandat 5600.709 kb on - strandat 5600.755 kb on - strandat 5600.789 kb on + strandat 5600.790 kb on - strand, within BT4250at 5600.814 kb on - strand, within BT4250at 5600.832 kb on - strand, within BT4250at 5600.834 kb on - strand, within BT4250at 5600.863 kb on - strand, within BT4250at 5600.947 kb on + strand, within BT4250at 5600.953 kb on + strand, within BT4250at 5600.956 kb on + strand, within BT4250at 5600.960 kb on - strand, within BT4250at 5600.962 kb on - strand, within BT4250at 5600.962 kb on - strand, within BT4250at 5601.042 kb on + strand, within BT4250at 5601.043 kb on - strand, within BT4250at 5601.077 kb on + strand, within BT4250at 5601.077 kb on + strand, within BT4250at 5601.077 kb on + strand, within BT4250at 5601.077 kb on + strand, within BT4250at 5601.078 kb on - strand, within BT4250

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM
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5,598,443 - BT4247 0.72 -1.9
5,598,467 + BT4247 0.72 -3.1
5,598,467 + BT4247 0.72 -1.3
5,598,492 + BT4247 0.73 -0.8
5,598,534 + BT4247 0.74 -0.9
5,598,535 - BT4247 0.74 -1.9
5,598,551 - BT4247 0.75 -2.2
5,598,551 - BT4247 0.75 +0.1
5,598,556 + BT4247 0.75 -3.6
5,598,556 + BT4247 0.75 -3.3
5,598,557 - BT4247 0.75 -4.1
5,598,735 + BT4247 0.80 -1.2
5,598,744 + BT4247 0.81 -1.4
5,598,744 + BT4247 0.81 +0.1
5,598,745 - BT4247 0.81 -1.4
5,598,873 + BT4247 0.84 -0.5
5,598,885 - BT4247 0.85 -1.4
5,598,987 + BT4247 0.88 +0.1
5,598,987 + BT4247 0.88 -1.3
5,599,022 - BT4247 0.89 -2.6
5,599,024 - -2.5
5,599,112 + +0.1
5,599,113 - +0.1
5,599,117 - -1.2
5,599,159 - -1.3
5,599,214 + -2.4
5,599,215 - +3.2
5,599,215 - -0.3
5,599,245 + -2.4
5,599,263 + +0.1
5,599,263 + +1.7
5,599,267 - -2.9
5,599,292 - -1.7
5,599,292 - +0.1
5,599,292 - -0.9
5,599,466 + -0.7
5,599,509 - BT4248 0.10 -1.4
5,599,509 - BT4248 0.10 -0.1
5,599,512 + BT4248 0.11 -0.9
5,599,698 + BT4248 0.39 +0.1
5,599,723 + BT4248 0.43 +2.2
5,599,723 + BT4248 0.43 +0.1
5,599,724 - BT4248 0.43 -1.4
5,599,728 + BT4248 0.44 +2.8
5,599,729 - BT4248 0.44 -1.3
5,599,766 + BT4248 0.49 -0.9
5,599,774 - BT4248 0.51 -0.9
5,599,866 - BT4248 0.65 +0.1
5,599,877 + BT4248 0.66 +0.9
5,599,910 + BT4248 0.71 -3.0
5,599,912 + BT4248 0.72 -2.8
5,599,944 + BT4248 0.77 -1.7
5,599,989 - BT4248 0.83 -2.3
5,600,013 - BT4248 0.87 -0.1
5,600,013 - BT4248 0.87 -1.5
5,600,109 + -2.5
5,600,170 - +1.1
5,600,236 - -0.9
5,600,322 + -0.9
5,600,338 + -0.7
5,600,355 - +0.2
5,600,359 + -1.7
5,600,360 - +1.1
5,600,392 + BT4249 0.12 +1.1
5,600,438 + BT4249 0.32 +0.1
5,600,476 - BT4249 0.48 -2.3
5,600,608 - -1.1
5,600,652 - -1.7
5,600,709 - -1.3
5,600,755 - -1.2
5,600,789 + +1.7
5,600,790 - BT4250 0.12 -0.9
5,600,814 - BT4250 0.16 -1.5
5,600,832 - BT4250 0.19 -1.5
5,600,834 - BT4250 0.20 +0.5
5,600,863 - BT4250 0.25 -2.0
5,600,947 + BT4250 0.40 +3.4
5,600,953 + BT4250 0.42 -1.5
5,600,956 + BT4250 0.42 -1.3
5,600,960 - BT4250 0.43 -3.0
5,600,962 - BT4250 0.43 -1.4
5,600,962 - BT4250 0.43 +0.2
5,601,042 + BT4250 0.58 -2.5
5,601,043 - BT4250 0.58 -2.9
5,601,077 + BT4250 0.64 -1.0
5,601,077 + BT4250 0.64 +2.2
5,601,077 + BT4250 0.64 -0.5
5,601,077 + BT4250 0.64 -1.5
5,601,078 - BT4250 0.64 -2.0

Or see this region's nucleotide sequence