Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3995 and BT3996 are separated by 107 nucleotides BT3996 and BT3997 are separated by 21 nucleotides BT3997 and BT3998 are separated by 1 nucleotides
BT3995: BT3995 - alanyl-tRNA synthetase (NCBI ptt file), at 5,205,400 to 5,208,018
BT3995
BT3996: BT3996 - putative peptidase (NCBI ptt file), at 5,208,126 to 5,209,094
BT3996
BT3997: BT3997 - transcriptional regulator (NCBI ptt file), at 5,209,116 to 5,209,457
BT3997
BT3998: BT3998 - GTP pyrophosphokinase (NCBI ptt file), at 5,209,459 to 5,211,702
BT3998
Position (kb)
5208
5209
5210 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5208.168 kb on + strand at 5208.168 kb on + strand at 5208.335 kb on + strand, within BT3996 at 5208.335 kb on + strand, within BT3996 at 5208.335 kb on + strand, within BT3996 at 5208.408 kb on + strand, within BT3996 at 5208.520 kb on + strand, within BT3996 at 5208.544 kb on + strand, within BT3996 at 5208.561 kb on + strand, within BT3996 at 5208.596 kb on + strand, within BT3996 at 5208.596 kb on + strand, within BT3996 at 5208.768 kb on + strand, within BT3996 at 5208.793 kb on + strand, within BT3996 at 5208.882 kb on + strand, within BT3996 at 5208.882 kb on + strand, within BT3996 at 5208.884 kb on + strand, within BT3996 at 5208.941 kb on + strand, within BT3996 at 5208.941 kb on + strand, within BT3996 at 5209.014 kb on + strand at 5209.014 kb on + strand at 5209.014 kb on + strand at 5209.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 5,208,168 + -0.1 5,208,168 + +2.4 5,208,335 + BT3996 0.22 -3.7 5,208,335 + BT3996 0.22 -3.1 5,208,335 + BT3996 0.22 -3.6 5,208,408 + BT3996 0.29 -0.6 5,208,520 + BT3996 0.41 -1.3 5,208,544 + BT3996 0.43 -0.1 5,208,561 + BT3996 0.45 -2.9 5,208,596 + BT3996 0.49 -3.4 5,208,596 + BT3996 0.49 -0.1 5,208,768 + BT3996 0.66 -2.9 5,208,793 + BT3996 0.69 -2.4 5,208,882 + BT3996 0.78 -3.8 5,208,882 + BT3996 0.78 -2.9 5,208,884 + BT3996 0.78 -2.2 5,208,941 + BT3996 0.84 -1.6 5,208,941 + BT3996 0.84 -2.6 5,209,014 + -0.1 5,209,014 + -1.1 5,209,014 + -2.5 5,209,044 + -4.0
Or see this region's nucleotide sequence