Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3310 and BT3311 are separated by 14 nucleotides BT3311 and BT3312 are separated by 58 nucleotides
BT3310: BT3310 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,248,560 to 4,251,565
BT3310
BT3311: BT3311 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,251,580 to 4,253,100
BT3311
BT3312: BT3312 - glucosylceramidase precursor (NCBI ptt file), at 4,253,159 to 4,254,649
BT3312
Position (kb)
4251
4252
4253
4254 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4250.588 kb on - strand, within BT3310 at 4250.667 kb on - strand, within BT3310 at 4250.669 kb on + strand, within BT3310 at 4250.670 kb on - strand, within BT3310 at 4250.673 kb on - strand, within BT3310 at 4250.704 kb on - strand, within BT3310 at 4250.782 kb on + strand, within BT3310 at 4250.891 kb on + strand, within BT3310 at 4250.913 kb on + strand, within BT3310 at 4250.913 kb on + strand, within BT3310 at 4250.929 kb on - strand, within BT3310 at 4250.975 kb on - strand, within BT3310 at 4251.009 kb on + strand, within BT3310 at 4251.010 kb on - strand, within BT3310 at 4251.038 kb on - strand, within BT3310 at 4251.062 kb on + strand, within BT3310 at 4251.063 kb on - strand, within BT3310 at 4251.063 kb on - strand, within BT3310 at 4251.166 kb on + strand, within BT3310 at 4251.204 kb on - strand, within BT3310 at 4251.204 kb on - strand, within BT3310 at 4251.262 kb on - strand, within BT3310 at 4251.281 kb on - strand at 4251.302 kb on - strand at 4251.306 kb on - strand at 4251.357 kb on - strand at 4251.357 kb on - strand at 4251.415 kb on + strand at 4251.446 kb on + strand at 4251.514 kb on + strand at 4251.514 kb on + strand at 4251.515 kb on - strand at 4251.536 kb on + strand at 4251.537 kb on - strand at 4251.551 kb on - strand at 4251.587 kb on + strand at 4251.671 kb on - strand at 4251.756 kb on + strand, within BT3311 at 4251.761 kb on - strand, within BT3311 at 4251.761 kb on - strand, within BT3311 at 4251.761 kb on - strand, within BT3311 at 4251.761 kb on - strand, within BT3311 at 4251.793 kb on + strand, within BT3311 at 4251.864 kb on - strand, within BT3311 at 4251.967 kb on - strand, within BT3311 at 4251.987 kb on + strand, within BT3311 at 4252.018 kb on + strand, within BT3311 at 4252.019 kb on - strand, within BT3311 at 4252.019 kb on - strand, within BT3311 at 4252.037 kb on + strand, within BT3311 at 4252.038 kb on - strand, within BT3311 at 4252.068 kb on - strand, within BT3311 at 4252.095 kb on + strand, within BT3311 at 4252.096 kb on - strand, within BT3311 at 4252.096 kb on - strand, within BT3311 at 4252.096 kb on - strand, within BT3311 at 4252.103 kb on + strand, within BT3311 at 4252.116 kb on + strand, within BT3311 at 4252.117 kb on - strand, within BT3311 at 4252.128 kb on + strand, within BT3311 at 4252.438 kb on - strand, within BT3311 at 4252.622 kb on + strand, within BT3311 at 4252.622 kb on + strand, within BT3311 at 4252.627 kb on + strand, within BT3311 at 4252.630 kb on + strand, within BT3311 at 4252.631 kb on - strand, within BT3311 at 4252.701 kb on + strand, within BT3311 at 4252.713 kb on + strand, within BT3311 at 4252.739 kb on + strand, within BT3311 at 4252.794 kb on + strand, within BT3311 at 4252.956 kb on - strand at 4252.976 kb on + strand at 4252.976 kb on + strand at 4252.978 kb on + strand at 4253.124 kb on - strand at 4253.124 kb on - strand at 4253.126 kb on - strand at 4253.163 kb on + strand at 4253.176 kb on + strand at 4253.209 kb on - strand at 4253.227 kb on + strand at 4253.330 kb on - strand, within BT3312 at 4253.419 kb on + strand, within BT3312 at 4253.420 kb on - strand, within BT3312 at 4253.431 kb on + strand, within BT3312 at 4253.523 kb on - strand, within BT3312 at 4253.523 kb on - strand, within BT3312 at 4253.567 kb on + strand, within BT3312 at 4253.569 kb on + strand, within BT3312 at 4253.569 kb on + strand, within BT3312 at 4253.570 kb on - strand, within BT3312 at 4253.628 kb on - strand, within BT3312 at 4253.629 kb on + strand, within BT3312 at 4253.679 kb on + strand, within BT3312 at 4253.780 kb on + strand, within BT3312 at 4253.781 kb on - strand, within BT3312 at 4253.793 kb on - strand, within BT3312 at 4253.802 kb on - strand, within BT3312 at 4253.850 kb on - strand, within BT3312 at 4253.850 kb on - strand, within BT3312 at 4253.857 kb on - strand, within BT3312 at 4253.903 kb on + strand, within BT3312 at 4254.011 kb on + strand, within BT3312 at 4254.039 kb on - strand, within BT3312 at 4254.100 kb on + strand, within BT3312
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 4,250,588 - BT3310 0.67 +2.7 4,250,667 - BT3310 0.70 -2.4 4,250,669 + BT3310 0.70 -2.1 4,250,670 - BT3310 0.70 +1.5 4,250,673 - BT3310 0.70 -1.3 4,250,704 - BT3310 0.71 -1.7 4,250,782 + BT3310 0.74 -1.1 4,250,891 + BT3310 0.78 +2.2 4,250,913 + BT3310 0.78 -2.7 4,250,913 + BT3310 0.78 -2.2 4,250,929 - BT3310 0.79 -1.7 4,250,975 - BT3310 0.80 -2.1 4,251,009 + BT3310 0.81 -1.1 4,251,010 - BT3310 0.82 +0.5 4,251,038 - BT3310 0.82 -1.7 4,251,062 + BT3310 0.83 -0.1 4,251,063 - BT3310 0.83 -0.0 4,251,063 - BT3310 0.83 +2.9 4,251,166 + BT3310 0.87 -2.3 4,251,204 - BT3310 0.88 -0.9 4,251,204 - BT3310 0.88 -0.6 4,251,262 - BT3310 0.90 -2.7 4,251,281 - -1.7 4,251,302 - -1.3 4,251,306 - -1.1 4,251,357 - -1.7 4,251,357 - -1.1 4,251,415 + +1.0 4,251,446 + -3.4 4,251,514 + -0.3 4,251,514 + -1.1 4,251,515 - -0.6 4,251,536 + -3.6 4,251,537 - -0.1 4,251,551 - -0.1 4,251,587 + -4.1 4,251,671 - -1.5 4,251,756 + BT3311 0.12 -1.4 4,251,761 - BT3311 0.12 -1.7 4,251,761 - BT3311 0.12 -1.7 4,251,761 - BT3311 0.12 -2.4 4,251,761 - BT3311 0.12 -0.1 4,251,793 + BT3311 0.14 -0.1 4,251,864 - BT3311 0.19 -1.1 4,251,967 - BT3311 0.25 -0.6 4,251,987 + BT3311 0.27 -2.3 4,252,018 + BT3311 0.29 -2.5 4,252,019 - BT3311 0.29 -0.1 4,252,019 - BT3311 0.29 -2.7 4,252,037 + BT3311 0.30 -2.1 4,252,038 - BT3311 0.30 -2.9 4,252,068 - BT3311 0.32 -0.3 4,252,095 + BT3311 0.34 -1.1 4,252,096 - BT3311 0.34 -1.1 4,252,096 - BT3311 0.34 -1.7 4,252,096 - BT3311 0.34 -3.1 4,252,103 + BT3311 0.34 -2.3 4,252,116 + BT3311 0.35 -2.4 4,252,117 - BT3311 0.35 -1.8 4,252,128 + BT3311 0.36 -2.4 4,252,438 - BT3311 0.56 -1.7 4,252,622 + BT3311 0.69 +0.0 4,252,622 + BT3311 0.69 -2.1 4,252,627 + BT3311 0.69 -0.1 4,252,630 + BT3311 0.69 +0.9 4,252,631 - BT3311 0.69 -1.7 4,252,701 + BT3311 0.74 -2.1 4,252,713 + BT3311 0.74 -2.1 4,252,739 + BT3311 0.76 -1.1 4,252,794 + BT3311 0.80 -1.7 4,252,956 - -0.3 4,252,976 + -0.1 4,252,976 + -2.6 4,252,978 + -0.1 4,253,124 - -1.2 4,253,124 - -3.4 4,253,126 - +1.5 4,253,163 + +0.5 4,253,176 + -1.4 4,253,209 - +0.9 4,253,227 + -0.8 4,253,330 - BT3312 0.11 +0.6 4,253,419 + BT3312 0.17 -0.1 4,253,420 - BT3312 0.18 -0.8 4,253,431 + BT3312 0.18 -2.0 4,253,523 - BT3312 0.24 -1.1 4,253,523 - BT3312 0.24 -2.1 4,253,567 + BT3312 0.27 -1.7 4,253,569 + BT3312 0.27 -1.7 4,253,569 + BT3312 0.27 -0.1 4,253,570 - BT3312 0.28 -1.8 4,253,628 - BT3312 0.31 -2.0 4,253,629 + BT3312 0.32 -0.6 4,253,679 + BT3312 0.35 -0.1 4,253,780 + BT3312 0.42 -2.4 4,253,781 - BT3312 0.42 -3.2 4,253,793 - BT3312 0.43 -2.6 4,253,802 - BT3312 0.43 -0.1 4,253,850 - BT3312 0.46 +2.9 4,253,850 - BT3312 0.46 -0.4 4,253,857 - BT3312 0.47 -0.1 4,253,903 + BT3312 0.50 -3.0 4,254,011 + BT3312 0.57 -0.9 4,254,039 - BT3312 0.59 -2.9 4,254,100 + BT3312 0.63 -2.4
Or see this region's nucleotide sequence