Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2245 and BT2246 are separated by 116 nucleotides BT2246 and BT2247 are separated by 3 nucleotides BT2247 and BT2248 are separated by 466 nucleotides
BT2245: BT2245 - hypothetical protein (NCBI ptt file), at 2,813,785 to 2,814,744
BT2245
BT2246: BT2246 - hypothetical protein (NCBI ptt file), at 2,814,861 to 2,816,909
BT2246
BT2247: BT2247 - putative ryanodine receptor (NCBI ptt file), at 2,816,913 to 2,817,215
BT2247
BT2248: BT2248 - putative integral membrane protein (NCBI ptt file), at 2,817,682 to 2,818,536
BT2248
Position (kb)
2814
2815
2816
2817 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 2813.878 kb on + strand at 2813.958 kb on + strand, within BT2245 at 2814.048 kb on - strand at 2814.141 kb on + strand, within BT2245 at 2814.141 kb on + strand, within BT2245 at 2814.142 kb on - strand, within BT2245 at 2814.209 kb on + strand, within BT2245 at 2814.316 kb on + strand, within BT2245 at 2814.466 kb on + strand, within BT2245 at 2814.544 kb on + strand, within BT2245 at 2814.545 kb on - strand, within BT2245 at 2814.601 kb on - strand, within BT2245 at 2814.601 kb on - strand, within BT2245 at 2814.726 kb on + strand at 2814.840 kb on + strand at 2815.016 kb on - strand at 2815.049 kb on + strand at 2815.050 kb on - strand at 2815.200 kb on - strand, within BT2246 at 2815.209 kb on - strand, within BT2246 at 2815.248 kb on + strand, within BT2246 at 2815.313 kb on - strand, within BT2246 at 2815.336 kb on + strand, within BT2246 at 2815.336 kb on + strand, within BT2246 at 2815.526 kb on - strand, within BT2246 at 2815.640 kb on + strand, within BT2246 at 2815.665 kb on + strand, within BT2246 at 2815.665 kb on + strand, within BT2246 at 2815.666 kb on - strand, within BT2246 at 2815.666 kb on - strand, within BT2246 at 2815.666 kb on - strand, within BT2246 at 2815.666 kb on - strand, within BT2246 at 2815.666 kb on - strand, within BT2246 at 2815.696 kb on + strand, within BT2246 at 2815.697 kb on - strand, within BT2246 at 2815.697 kb on - strand, within BT2246 at 2815.743 kb on + strand, within BT2246 at 2815.756 kb on + strand, within BT2246 at 2815.757 kb on - strand, within BT2246 at 2815.762 kb on - strand, within BT2246 at 2815.780 kb on + strand, within BT2246 at 2815.781 kb on - strand, within BT2246 at 2815.881 kb on + strand, within BT2246 at 2815.882 kb on - strand, within BT2246 at 2815.948 kb on + strand, within BT2246 at 2815.963 kb on + strand, within BT2246 at 2816.080 kb on + strand, within BT2246 at 2816.090 kb on + strand, within BT2246 at 2816.149 kb on - strand, within BT2246 at 2816.276 kb on + strand, within BT2246 at 2816.277 kb on - strand, within BT2246 at 2816.303 kb on - strand at 2816.389 kb on - strand, within BT2246 at 2816.461 kb on - strand, within BT2246 at 2816.521 kb on - strand, within BT2246 at 2816.526 kb on - strand, within BT2246 at 2816.631 kb on - strand, within BT2246 at 2816.661 kb on + strand, within BT2246 at 2816.662 kb on - strand, within BT2246 at 2816.693 kb on + strand, within BT2246 at 2816.700 kb on + strand, within BT2246 at 2816.786 kb on - strand at 2816.839 kb on - strand at 2816.841 kb on - strand at 2816.941 kb on - strand at 2817.047 kb on + strand, within BT2247 at 2817.216 kb on - strand at 2817.237 kb on + strand at 2817.242 kb on + strand at 2817.246 kb on - strand at 2817.318 kb on - strand at 2817.325 kb on - strand at 2817.326 kb on + strand at 2817.355 kb on + strand at 2817.402 kb on - strand at 2817.466 kb on - strand at 2817.476 kb on - strand at 2817.547 kb on - strand at 2817.548 kb on + strand at 2817.551 kb on + strand at 2817.551 kb on + strand at 2817.552 kb on - strand at 2817.552 kb on - strand at 2817.588 kb on + strand at 2817.677 kb on - strand at 2817.677 kb on - strand at 2817.709 kb on + strand at 2817.709 kb on + strand at 2817.709 kb on + strand at 2817.709 kb on + strand at 2817.771 kb on - strand, within BT2248 at 2817.839 kb on + strand, within BT2248 at 2817.896 kb on - strand, within BT2248 at 2817.897 kb on + strand, within BT2248 at 2817.897 kb on + strand, within BT2248 at 2817.898 kb on - strand, within BT2248
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 2,813,878 + +1.2 2,813,958 + BT2245 0.18 -3.1 2,814,048 - +0.0 2,814,141 + BT2245 0.37 -1.7 2,814,141 + BT2245 0.37 +2.3 2,814,142 - BT2245 0.37 -1.0 2,814,209 + BT2245 0.44 +2.6 2,814,316 + BT2245 0.55 -1.1 2,814,466 + BT2245 0.71 -3.0 2,814,544 + BT2245 0.79 +0.0 2,814,545 - BT2245 0.79 +0.5 2,814,601 - BT2245 0.85 -1.7 2,814,601 - BT2245 0.85 -2.6 2,814,726 + -0.4 2,814,840 + -1.8 2,815,016 - -1.6 2,815,049 + -0.2 2,815,050 - +0.0 2,815,200 - BT2246 0.17 -1.6 2,815,209 - BT2246 0.17 -1.6 2,815,248 + BT2246 0.19 -2.6 2,815,313 - BT2246 0.22 -0.0 2,815,336 + BT2246 0.23 -0.0 2,815,336 + BT2246 0.23 +5.8 2,815,526 - BT2246 0.32 -3.0 2,815,640 + BT2246 0.38 -2.0 2,815,665 + BT2246 0.39 -2.8 2,815,665 + BT2246 0.39 -1.2 2,815,666 - BT2246 0.39 -0.0 2,815,666 - BT2246 0.39 +2.0 2,815,666 - BT2246 0.39 -3.3 2,815,666 - BT2246 0.39 -4.3 2,815,666 - BT2246 0.39 -2.0 2,815,696 + BT2246 0.41 -3.9 2,815,697 - BT2246 0.41 -1.0 2,815,697 - BT2246 0.41 -1.0 2,815,743 + BT2246 0.43 -1.3 2,815,756 + BT2246 0.44 +1.0 2,815,757 - BT2246 0.44 +1.4 2,815,762 - BT2246 0.44 -2.0 2,815,780 + BT2246 0.45 -1.0 2,815,781 - BT2246 0.45 -0.6 2,815,881 + BT2246 0.50 -0.7 2,815,882 - BT2246 0.50 +1.8 2,815,948 + BT2246 0.53 -0.0 2,815,963 + BT2246 0.54 -1.6 2,816,080 + BT2246 0.59 -2.2 2,816,090 + BT2246 0.60 -3.7 2,816,149 - BT2246 0.63 -1.6 2,816,276 + BT2246 0.69 -0.8 2,816,277 - BT2246 0.69 +0.7 2,816,303 - -0.0 2,816,389 - BT2246 0.75 -1.8 2,816,461 - BT2246 0.78 -2.0 2,816,521 - BT2246 0.81 -0.5 2,816,526 - BT2246 0.81 -1.6 2,816,631 - BT2246 0.86 -0.4 2,816,661 + BT2246 0.88 -1.0 2,816,662 - BT2246 0.88 -0.0 2,816,693 + BT2246 0.89 -1.3 2,816,700 + BT2246 0.90 -0.2 2,816,786 - -1.4 2,816,839 - -0.0 2,816,841 - -0.9 2,816,941 - -0.6 2,817,047 + BT2247 0.44 -2.2 2,817,216 - +1.0 2,817,237 + +0.3 2,817,242 + -1.1 2,817,246 - -2.6 2,817,318 - -1.0 2,817,325 - -1.8 2,817,326 + +2.3 2,817,355 + -0.0 2,817,402 - +2.0 2,817,466 - -2.3 2,817,476 - -3.0 2,817,547 - +0.4 2,817,548 + -1.9 2,817,551 + -2.4 2,817,551 + -1.3 2,817,552 - -1.3 2,817,552 - -3.3 2,817,588 + -3.2 2,817,677 - +0.0 2,817,677 - +0.0 2,817,709 + -3.1 2,817,709 + +1.6 2,817,709 + -2.0 2,817,709 + -1.6 2,817,771 - BT2248 0.10 -2.8 2,817,839 + BT2248 0.18 -3.7 2,817,896 - BT2248 0.25 -2.7 2,817,897 + BT2248 0.25 -1.5 2,817,897 + BT2248 0.25 -2.3 2,817,898 - BT2248 0.25 +0.6
Or see this region's nucleotide sequence