Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Ser and BT2056 are separated by 120 nucleotides BT2056 and BT2057 overlap by 7 nucleotides BT2057 and BT2058 are separated by 15 nucleotides
BT_t34: tRNA-Ser - tRNA-Ser (RefSeq), at 2,575,512 to 2,575,599
tRNA-Ser
BT2056: BT2056 - conserved hypothetical protein (NCBI ptt file), at 2,575,720 to 2,576,496
BT2056
BT2057: BT2057 - hypothetical protein (NCBI ptt file), at 2,576,490 to 2,577,206
BT2057
BT2058: BT2058 - putative isoprenyl synthetase (NCBI ptt file), at 2,577,222 to 2,578,196
BT2058
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2575.553 kb on - strand, within tRNA-Ser at 2575.681 kb on - strand at 2575.681 kb on - strand at 2575.699 kb on + strand at 2575.818 kb on + strand, within BT2056 at 2575.819 kb on - strand, within BT2056 at 2576.079 kb on + strand, within BT2056 at 2576.082 kb on - strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.085 kb on + strand, within BT2056 at 2576.087 kb on + strand, within BT2056 at 2576.127 kb on + strand, within BT2056 at 2576.127 kb on + strand, within BT2056 at 2576.227 kb on + strand, within BT2056 at 2576.235 kb on + strand, within BT2056 at 2576.236 kb on - strand, within BT2056 at 2576.301 kb on + strand, within BT2056 at 2576.394 kb on + strand, within BT2056 at 2576.400 kb on + strand, within BT2056 at 2576.484 kb on + strand at 2576.484 kb on + strand at 2576.488 kb on + strand at 2576.489 kb on - strand at 2576.539 kb on + strand at 2576.543 kb on - strand at 2576.719 kb on + strand, within BT2057 at 2576.719 kb on + strand, within BT2057 at 2576.740 kb on + strand, within BT2057 at 2576.752 kb on - strand, within BT2057 at 2576.773 kb on + strand, within BT2057 at 2576.893 kb on + strand, within BT2057 at 2576.947 kb on - strand, within BT2057 at 2576.947 kb on - strand, within BT2057 at 2576.966 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.969 kb on - strand, within BT2057 at 2577.038 kb on + strand, within BT2057 at 2577.125 kb on + strand, within BT2057 at 2577.128 kb on - strand, within BT2057 at 2577.165 kb on + strand at 2577.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 2,575,553 - tRNA-Ser BT_t34 0.47 -0.9 2,575,681 - -0.9 2,575,681 - +1.1 2,575,699 + +6.2 2,575,818 + BT2056 0.13 -1.2 2,575,819 - BT2056 0.13 -1.9 2,576,079 + BT2056 0.46 -2.5 2,576,082 - BT2056 0.47 +0.1 2,576,083 + BT2056 0.47 -2.3 2,576,083 + BT2056 0.47 -1.6 2,576,083 + BT2056 0.47 -1.5 2,576,085 + BT2056 0.47 -0.7 2,576,087 + BT2056 0.47 -1.3 2,576,127 + BT2056 0.52 -1.7 2,576,127 + BT2056 0.52 -2.2 2,576,227 + BT2056 0.65 -2.1 2,576,235 + BT2056 0.66 +1.6 2,576,236 - BT2056 0.66 -1.4 2,576,301 + BT2056 0.75 +0.1 2,576,394 + BT2056 0.87 -0.5 2,576,400 + BT2056 0.88 +2.1 2,576,484 + +0.6 2,576,484 + -1.9 2,576,488 + -2.5 2,576,489 - +0.1 2,576,539 + -0.4 2,576,543 - -3.2 2,576,719 + BT2057 0.32 +0.1 2,576,719 + BT2057 0.32 -0.4 2,576,740 + BT2057 0.35 -0.7 2,576,752 - BT2057 0.37 -0.5 2,576,773 + BT2057 0.39 +0.6 2,576,893 + BT2057 0.56 -2.4 2,576,947 - BT2057 0.64 +1.1 2,576,947 - BT2057 0.64 -1.5 2,576,966 + BT2057 0.66 -3.3 2,576,968 + BT2057 0.67 -0.7 2,576,968 + BT2057 0.67 -2.5 2,576,968 + BT2057 0.67 +1.1 2,576,969 - BT2057 0.67 +0.1 2,577,038 + BT2057 0.76 -0.9 2,577,125 + BT2057 0.89 +2.4 2,577,128 - BT2057 0.89 +1.6 2,577,165 + +1.1 2,577,200 - +0.1
Or see this region's nucleotide sequence