Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1412 and BT1413 are separated by 266 nucleotides BT1413 and BT1414 are separated by 89 nucleotides
BT1412: BT1412 - iron-sulfur flavoprotein (NCBI ptt file), at 1,745,411 to 1,745,950
BT1412
BT1413: BT1413 - putative transcription regulator (NCBI ptt file), at 1,746,217 to 1,746,906
BT1413
BT1414: BT1414 - hypothetical protein (NCBI ptt file), at 1,746,996 to 1,748,282
BT1414
Position (kb)
1746
1747 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1745.282 kb on + strand at 1745.282 kb on + strand at 1745.285 kb on - strand at 1745.298 kb on + strand at 1745.321 kb on - strand at 1745.338 kb on - strand at 1745.339 kb on + strand at 1745.340 kb on - strand at 1745.342 kb on - strand at 1745.351 kb on - strand at 1745.377 kb on + strand at 1745.378 kb on - strand at 1745.395 kb on + strand at 1745.433 kb on + strand at 1745.463 kb on - strand at 1745.486 kb on + strand, within BT1412 at 1745.656 kb on + strand, within BT1412 at 1745.683 kb on - strand, within BT1412 at 1745.779 kb on - strand, within BT1412 at 1745.794 kb on + strand, within BT1412 at 1745.795 kb on - strand, within BT1412 at 1745.893 kb on + strand, within BT1412 at 1746.024 kb on + strand at 1746.060 kb on - strand at 1746.177 kb on + strand at 1746.178 kb on - strand at 1746.185 kb on - strand at 1746.200 kb on - strand at 1746.210 kb on + strand at 1746.334 kb on + strand, within BT1413 at 1746.335 kb on - strand, within BT1413 at 1746.357 kb on - strand, within BT1413 at 1746.390 kb on - strand, within BT1413 at 1746.391 kb on + strand, within BT1413 at 1746.392 kb on - strand, within BT1413 at 1746.392 kb on - strand, within BT1413 at 1746.392 kb on - strand, within BT1413 at 1746.461 kb on - strand, within BT1413 at 1746.461 kb on - strand, within BT1413 at 1746.461 kb on - strand, within BT1413 at 1746.462 kb on + strand, within BT1413 at 1746.477 kb on + strand, within BT1413 at 1746.555 kb on - strand, within BT1413 at 1746.583 kb on - strand, within BT1413 at 1746.645 kb on + strand, within BT1413 at 1746.646 kb on - strand, within BT1413 at 1746.646 kb on - strand, within BT1413 at 1746.647 kb on + strand, within BT1413 at 1746.647 kb on + strand, within BT1413 at 1746.651 kb on + strand, within BT1413 at 1746.652 kb on - strand, within BT1413 at 1746.689 kb on + strand, within BT1413 at 1746.702 kb on + strand, within BT1413 at 1746.709 kb on + strand, within BT1413 at 1746.709 kb on + strand, within BT1413 at 1746.759 kb on + strand, within BT1413 at 1746.760 kb on - strand, within BT1413 at 1746.792 kb on - strand, within BT1413 at 1746.935 kb on - strand at 1746.959 kb on + strand at 1746.960 kb on - strand at 1746.982 kb on + strand at 1747.097 kb on + strand at 1747.212 kb on + strand, within BT1414 at 1747.253 kb on + strand, within BT1414 at 1747.254 kb on - strand, within BT1414 at 1747.258 kb on - strand, within BT1414 at 1747.300 kb on - strand, within BT1414 at 1747.340 kb on - strand, within BT1414 at 1747.340 kb on - strand, within BT1414 at 1747.407 kb on - strand, within BT1414 at 1747.436 kb on - strand, within BT1414 at 1747.532 kb on + strand, within BT1414 at 1747.581 kb on - strand, within BT1414 at 1747.581 kb on - strand, within BT1414 at 1747.584 kb on + strand, within BT1414 at 1747.586 kb on + strand, within BT1414 at 1747.592 kb on - strand, within BT1414 at 1747.668 kb on - strand, within BT1414 at 1747.706 kb on + strand, within BT1414 at 1747.706 kb on + strand, within BT1414 at 1747.706 kb on + strand, within BT1414 at 1747.708 kb on + strand, within BT1414 at 1747.738 kb on + strand, within BT1414 at 1747.763 kb on - strand, within BT1414 at 1747.765 kb on + strand, within BT1414 at 1747.765 kb on + strand, within BT1414 at 1747.811 kb on + strand, within BT1414 at 1747.812 kb on - strand, within BT1414 at 1747.818 kb on + strand, within BT1414
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 1,745,282 + -4.9 1,745,282 + -2.7 1,745,285 - -0.9 1,745,298 + -0.9 1,745,321 - -0.9 1,745,338 - -0.3 1,745,339 + +0.1 1,745,340 - -0.5 1,745,342 - -1.5 1,745,351 - -2.2 1,745,377 + -0.9 1,745,378 - +0.1 1,745,395 + +0.1 1,745,433 + -1.6 1,745,463 - -3.6 1,745,486 + BT1412 0.14 -0.9 1,745,656 + BT1412 0.45 +0.1 1,745,683 - BT1412 0.50 -0.2 1,745,779 - BT1412 0.68 -2.6 1,745,794 + BT1412 0.71 +0.1 1,745,795 - BT1412 0.71 +1.7 1,745,893 + BT1412 0.89 -0.9 1,746,024 + +1.7 1,746,060 - +0.4 1,746,177 + -0.9 1,746,178 - +0.1 1,746,185 - -2.2 1,746,200 - +2.4 1,746,210 + -3.1 1,746,334 + BT1413 0.17 -2.8 1,746,335 - BT1413 0.17 -1.5 1,746,357 - BT1413 0.20 -2.2 1,746,390 - BT1413 0.25 +0.2 1,746,391 + BT1413 0.25 -2.7 1,746,392 - BT1413 0.25 -1.7 1,746,392 - BT1413 0.25 -3.2 1,746,392 - BT1413 0.25 +0.9 1,746,461 - BT1413 0.35 +1.7 1,746,461 - BT1413 0.35 -0.5 1,746,461 - BT1413 0.35 +0.2 1,746,462 + BT1413 0.36 -1.5 1,746,477 + BT1413 0.38 +1.7 1,746,555 - BT1413 0.49 +1.1 1,746,583 - BT1413 0.53 +0.1 1,746,645 + BT1413 0.62 +1.1 1,746,646 - BT1413 0.62 -0.5 1,746,646 - BT1413 0.62 +0.1 1,746,647 + BT1413 0.62 -3.1 1,746,647 + BT1413 0.62 -0.8 1,746,651 + BT1413 0.63 -1.6 1,746,652 - BT1413 0.63 -4.1 1,746,689 + BT1413 0.68 -0.9 1,746,702 + BT1413 0.70 -0.9 1,746,709 + BT1413 0.71 -0.7 1,746,709 + BT1413 0.71 -0.9 1,746,759 + BT1413 0.79 -1.5 1,746,760 - BT1413 0.79 -2.3 1,746,792 - BT1413 0.83 -2.5 1,746,935 - -0.3 1,746,959 + -0.5 1,746,960 - -1.6 1,746,982 + +0.1 1,747,097 + +0.1 1,747,212 + BT1414 0.17 -1.9 1,747,253 + BT1414 0.20 -2.2 1,747,254 - BT1414 0.20 +1.7 1,747,258 - BT1414 0.20 +1.1 1,747,300 - BT1414 0.24 -2.2 1,747,340 - BT1414 0.27 +0.1 1,747,340 - BT1414 0.27 -1.5 1,747,407 - BT1414 0.32 -1.7 1,747,436 - BT1414 0.34 -0.9 1,747,532 + BT1414 0.42 -2.2 1,747,581 - BT1414 0.45 -3.1 1,747,581 - BT1414 0.45 -2.5 1,747,584 + BT1414 0.46 -0.9 1,747,586 + BT1414 0.46 -1.9 1,747,592 - BT1414 0.46 -2.8 1,747,668 - BT1414 0.52 -2.0 1,747,706 + BT1414 0.55 +0.4 1,747,706 + BT1414 0.55 +0.7 1,747,706 + BT1414 0.55 -2.3 1,747,708 + BT1414 0.55 -2.7 1,747,738 + BT1414 0.58 -2.5 1,747,763 - BT1414 0.60 -2.2 1,747,765 + BT1414 0.60 -1.8 1,747,765 + BT1414 0.60 +0.1 1,747,811 + BT1414 0.63 -1.5 1,747,812 - BT1414 0.63 -1.9 1,747,818 + BT1414 0.64 +0.1
Or see this region's nucleotide sequence