Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0432 and tRNA-Thr are separated by 66 nucleotides tRNA-Thr and BT0433 are separated by 129 nucleotides BT0433 and BT0434 are separated by 339 nucleotides
BT0432: BT0432 - putative endonuclease BB0411 (NCBI ptt file), at 519,272 to 520,150
BT0432
BT_t02: tRNA-Thr - tRNA-Thr (RefSeq), at 520,217 to 520,289
tRNA-Thr
BT0433: BT0433 - putative xylose repressor (NCBI ptt file), at 520,419 to 521,627
BT0433
BT0434: BT0434 - conserved hypothetical protein (NCBI ptt file), at 521,967 to 523,850
BT0434
Position (kb) 520
521
522 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 519.426 kb on + strand, within BT0432 at 519.428 kb on + strand, within BT0432 at 519.649 kb on + strand, within BT0432 at 519.989 kb on + strand, within BT0432 at 520.003 kb on + strand, within BT0432 at 520.004 kb on - strand, within BT0432 at 520.020 kb on + strand, within BT0432 at 520.032 kb on + strand, within BT0432 at 520.039 kb on - strand, within BT0432 at 520.060 kb on + strand, within BT0432 at 520.081 kb on + strand at 520.172 kb on + strand at 520.246 kb on + strand, within tRNA-Thr at 520.329 kb on - strand at 521.181 kb on - strand, within BT0433 at 522.048 kb on - strand at 522.053 kb on - strand at 522.062 kb on + strand at 522.065 kb on + strand at 522.290 kb on - strand, within BT0434 at 522.522 kb on + strand, within BT0434 at 522.587 kb on + strand, within BT0434 at 522.603 kb on - strand, within BT0434 at 522.620 kb on - strand, within BT0434
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 519,426 + BT0432 0.18 -1.0 519,428 + BT0432 0.18 -1.6 519,649 + BT0432 0.43 -0.8 519,989 + BT0432 0.82 -1.3 520,003 + BT0432 0.83 +0.0 520,004 - BT0432 0.83 -1.8 520,020 + BT0432 0.85 -2.6 520,032 + BT0432 0.86 -1.0 520,039 - BT0432 0.87 +0.0 520,060 + BT0432 0.90 +0.4 520,081 + +0.0 520,172 + -2.0 520,246 + tRNA-Thr BT_t02 0.40 -2.0 520,329 - -2.0 521,181 - BT0433 0.63 +0.0 522,048 - -2.7 522,053 - -2.4 522,062 + -1.6 522,065 + -3.8 522,290 - BT0434 0.17 +3.6 522,522 + BT0434 0.29 -2.7 522,587 + BT0434 0.33 -2.6 522,603 - BT0434 0.34 +0.6 522,620 - BT0434 0.35 -3.3
Or see this region's nucleotide sequence