Experiment: BHIS with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0005 and BT0006 are separated by 91 nucleotides BT0006 and BT0007 overlap by 78 nucleotides BT0007 and BT0008 are separated by 108 nucleotides BT0008 and BT0009 are separated by 128 nucleotides
BT0005: BT0005 - 30S ribosomal protein S17 (NCBI ptt file), at 3,460 to 3,996
BT0005
BT0006: BT0006 - hypothetical protein (NCBI ptt file), at 4,088 to 4,534
BT0006
BT0007: BT0007 - hypothetical protein (NCBI ptt file), at 4,457 to 4,849
BT0007
BT0008: BT0008 - conserved hypothetical protein (NCBI ptt file), at 4,958 to 5,503
BT0008
BT0009: BT0009 - phosphate-binding periplasmic protein precursor, putative ABC transporter (NCBI ptt file), at 5,632 to 6,741
BT0009
Position (kb) 4
5 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 3.458 kb on - strand at 3.550 kb on - strand, within BT0005 at 3.557 kb on + strand, within BT0005 at 3.638 kb on - strand, within BT0005 at 3.694 kb on - strand, within BT0005 at 3.696 kb on + strand, within BT0005 at 3.697 kb on - strand, within BT0005 at 3.746 kb on + strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.785 kb on + strand, within BT0005 at 3.872 kb on - strand, within BT0005 at 3.917 kb on + strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 4.104 kb on + strand at 4.208 kb on - strand, within BT0006 at 4.258 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.291 kb on - strand, within BT0006 at 4.301 kb on - strand, within BT0006 at 4.304 kb on + strand, within BT0006 at 4.324 kb on + strand, within BT0006 at 4.335 kb on - strand, within BT0006 at 4.364 kb on - strand, within BT0006 at 4.367 kb on + strand, within BT0006 at 4.486 kb on + strand at 4.567 kb on - strand, within BT0007 at 4.569 kb on - strand, within BT0007 at 4.571 kb on - strand, within BT0007 at 4.714 kb on - strand, within BT0007 at 4.714 kb on - strand, within BT0007 at 4.747 kb on + strand, within BT0007 at 4.969 kb on - strand at 5.078 kb on + strand, within BT0008 at 5.101 kb on + strand, within BT0008 at 5.105 kb on + strand, within BT0008 at 5.280 kb on + strand, within BT0008 at 5.296 kb on + strand, within BT0008 at 5.297 kb on - strand, within BT0008 at 5.350 kb on + strand, within BT0008 at 5.368 kb on + strand, within BT0008 at 5.475 kb on + strand at 5.480 kb on + strand at 5.519 kb on + strand at 5.520 kb on - strand at 5.631 kb on + strand at 5.643 kb on + strand at 5.676 kb on - strand at 5.676 kb on - strand at 5.721 kb on - strand at 5.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.025 mM remove 3,458 - +0.6 3,550 - BT0005 0.17 -2.8 3,557 + BT0005 0.18 -3.6 3,638 - BT0005 0.33 -3.0 3,694 - BT0005 0.44 -2.4 3,696 + BT0005 0.44 +0.0 3,697 - BT0005 0.44 -3.0 3,746 + BT0005 0.53 -1.0 3,747 - BT0005 0.53 +0.5 3,747 - BT0005 0.53 -0.8 3,785 + BT0005 0.61 -3.8 3,872 - BT0005 0.77 -0.5 3,917 + BT0005 0.85 -1.2 3,918 - BT0005 0.85 -2.1 3,918 - BT0005 0.85 -1.2 4,104 + -2.0 4,208 - BT0006 0.27 -1.7 4,258 - BT0006 0.38 -2.5 4,278 - BT0006 0.43 -2.5 4,278 - BT0006 0.43 -2.9 4,280 - BT0006 0.43 -1.7 4,280 - BT0006 0.43 -2.1 4,291 - BT0006 0.45 +1.0 4,301 - BT0006 0.48 -0.4 4,304 + BT0006 0.48 -2.0 4,324 + BT0006 0.53 -2.3 4,335 - BT0006 0.55 +0.0 4,364 - BT0006 0.62 -2.0 4,367 + BT0006 0.62 -1.7 4,486 + -2.4 4,567 - BT0007 0.28 +2.4 4,569 - BT0007 0.28 -3.7 4,571 - BT0007 0.29 +1.6 4,714 - BT0007 0.65 +0.0 4,714 - BT0007 0.65 +3.5 4,747 + BT0007 0.74 +0.0 4,969 - -1.1 5,078 + BT0008 0.22 -0.8 5,101 + BT0008 0.26 -1.0 5,105 + BT0008 0.27 -3.5 5,280 + BT0008 0.59 -1.5 5,296 + BT0008 0.62 -2.1 5,297 - BT0008 0.62 +3.7 5,350 + BT0008 0.72 -1.5 5,368 + BT0008 0.75 -1.0 5,475 + -0.5 5,480 + +1.0 5,519 + -0.8 5,520 - +0.0 5,631 + +1.0 5,643 + -2.3 5,676 - -1.3 5,676 - -1.7 5,721 - -1.6 5,725 - +0.0
Or see this region's nucleotide sequence