Experiment: MoLS4 with L-Glycine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3166 and DVU3167 are separated by 93 nucleotides DVU3167 and hemL are separated by 67 nucleotides hemL and cbiG are separated by 120 nucleotides
DVU3166: DVU3166 - alanyl-tRNA synthetase family protein, putative (TIGR), at 3,322,606 to 3,323,262
DVU3166
DVU3167: DVU3167 - heme biosynthesis protein, putative (TIGR), at 3,323,356 to 3,323,838
DVU3167
DVU3168: hemL - glutamate-1-semialdehyde-2,1-aminomutase (TIGR), at 3,323,906 to 3,325,177
hemL
DVU3169: cbiG - cobalamin biosynthesis protein CbiG (TIGR), at 3,325,298 to 3,326,494
cbiG
Position (kb)
3323
3324
3325
3326 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3323.033 kb on + strand, within DVU3166 at 3323.057 kb on + strand, within DVU3166 at 3323.065 kb on - strand, within DVU3166 at 3323.113 kb on - strand, within DVU3166 at 3323.113 kb on - strand, within DVU3166 at 3323.194 kb on - strand, within DVU3166 at 3323.281 kb on - strand at 3323.873 kb on + strand at 3325.179 kb on - strand at 3325.252 kb on + strand at 3325.252 kb on + strand at 3325.260 kb on - strand at 3325.296 kb on + strand at 3325.315 kb on + strand at 3325.375 kb on + strand at 3325.500 kb on + strand, within cbiG at 3325.526 kb on + strand, within cbiG at 3325.604 kb on + strand, within cbiG at 3325.620 kb on + strand, within cbiG at 3325.648 kb on + strand, within cbiG at 3325.675 kb on - strand, within cbiG at 3325.679 kb on + strand, within cbiG at 3325.687 kb on - strand, within cbiG at 3325.716 kb on - strand, within cbiG at 3325.716 kb on - strand, within cbiG at 3325.754 kb on - strand, within cbiG at 3325.754 kb on - strand, within cbiG at 3325.821 kb on + strand, within cbiG at 3325.821 kb on + strand, within cbiG at 3325.824 kb on - strand, within cbiG at 3325.892 kb on + strand, within cbiG at 3325.892 kb on + strand, within cbiG at 3325.892 kb on + strand, within cbiG at 3325.900 kb on - strand, within cbiG at 3325.905 kb on - strand, within cbiG at 3325.915 kb on - strand, within cbiG at 3325.978 kb on + strand, within cbiG at 3325.984 kb on - strand, within cbiG at 3325.997 kb on + strand, within cbiG at 3326.002 kb on + strand, within cbiG at 3326.039 kb on + strand, within cbiG at 3326.125 kb on - strand, within cbiG
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine as nutrient 10mM remove 3,323,033 + DVU3166 0.65 -0.3 3,323,057 + DVU3166 0.69 -0.0 3,323,065 - DVU3166 0.70 +0.2 3,323,113 - DVU3166 0.77 -0.1 3,323,113 - DVU3166 0.77 -0.1 3,323,194 - DVU3166 0.89 -0.1 3,323,281 - +1.1 3,323,873 + -0.1 3,325,179 - +0.3 3,325,252 + -2.2 3,325,252 + +1.1 3,325,260 - +0.0 3,325,296 + +0.6 3,325,315 + -0.3 3,325,375 + -2.4 3,325,500 + cbiG DVU3169 0.17 -4.2 3,325,526 + cbiG DVU3169 0.19 -1.9 3,325,604 + cbiG DVU3169 0.26 -2.1 3,325,620 + cbiG DVU3169 0.27 -3.5 3,325,648 + cbiG DVU3169 0.29 -1.1 3,325,675 - cbiG DVU3169 0.31 -3.2 3,325,679 + cbiG DVU3169 0.32 -2.8 3,325,687 - cbiG DVU3169 0.32 -2.2 3,325,716 - cbiG DVU3169 0.35 -3.4 3,325,716 - cbiG DVU3169 0.35 -1.6 3,325,754 - cbiG DVU3169 0.38 -5.0 3,325,754 - cbiG DVU3169 0.38 -4.5 3,325,821 + cbiG DVU3169 0.44 +0.9 3,325,821 + cbiG DVU3169 0.44 -3.4 3,325,824 - cbiG DVU3169 0.44 -2.5 3,325,892 + cbiG DVU3169 0.50 +0.7 3,325,892 + cbiG DVU3169 0.50 -0.5 3,325,892 + cbiG DVU3169 0.50 -1.3 3,325,900 - cbiG DVU3169 0.50 -4.0 3,325,905 - cbiG DVU3169 0.51 -4.6 3,325,915 - cbiG DVU3169 0.52 -2.8 3,325,978 + cbiG DVU3169 0.57 -1.5 3,325,984 - cbiG DVU3169 0.57 -1.0 3,325,997 + cbiG DVU3169 0.58 +0.7 3,326,002 + cbiG DVU3169 0.59 -2.0 3,326,039 + cbiG DVU3169 0.62 -4.1 3,326,125 - cbiG DVU3169 0.69 -0.7
Or see this region's nucleotide sequence