Experiment: MoLS4 with L-Glycine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt opuBB and proV overlap by 4 nucleotides proV and DVU2300 are separated by 135 nucleotides DVU2300 and DVU2301 are separated by 13 nucleotides DVU2301 and DVU2302 are separated by 11 nucleotides
DVU2298: opuBB - glycine/betaine/L-proline ABC transporter, permease protein (TIGR), at 2,392,146 to 2,392,997
opuBB
DVU2299: proV - glycine/betaine/L-proline ABC transporter, ATP binding protein (TIGR), at 2,392,994 to 2,394,187
proV
DVU2300: DVU2300 - hypothetical protein (TIGR), at 2,394,323 to 2,394,478
DVU2300
DVU2301: DVU2301 - lipoprotein, putative (TIGR), at 2,394,492 to 2,394,629
DVU2301
DVU2302: DVU2302 - glutathione-regulated potassium-efflux system protein KefB, putative (TIGR), at 2,394,641 to 2,396,659
DVU2302
Position (kb)
2392
2393
2394
2395 Strain fitness (log2 ratio)
-1
0
1
2 at 2392.002 kb on - strand at 2392.013 kb on + strand at 2392.029 kb on + strand at 2392.064 kb on - strand at 2392.247 kb on + strand, within opuBB at 2392.262 kb on - strand, within opuBB at 2392.299 kb on + strand, within opuBB at 2392.390 kb on + strand, within opuBB at 2392.390 kb on + strand, within opuBB at 2392.427 kb on + strand, within opuBB at 2392.450 kb on + strand, within opuBB at 2392.508 kb on + strand, within opuBB at 2392.592 kb on + strand, within opuBB at 2392.600 kb on - strand, within opuBB at 2392.653 kb on + strand, within opuBB at 2393.241 kb on - strand, within proV at 2393.241 kb on - strand, within proV at 2393.304 kb on + strand, within proV at 2393.314 kb on + strand, within proV at 2393.430 kb on + strand, within proV at 2393.438 kb on + strand, within proV at 2393.438 kb on + strand, within proV at 2393.440 kb on + strand, within proV at 2393.464 kb on - strand, within proV at 2393.469 kb on - strand, within proV at 2393.518 kb on + strand, within proV at 2393.533 kb on + strand, within proV at 2393.541 kb on - strand, within proV at 2393.620 kb on + strand, within proV at 2393.688 kb on + strand, within proV at 2393.696 kb on - strand, within proV at 2393.731 kb on + strand, within proV at 2393.739 kb on - strand, within proV at 2393.747 kb on - strand, within proV at 2393.793 kb on - strand, within proV at 2393.816 kb on + strand, within proV at 2393.816 kb on + strand, within proV at 2393.821 kb on + strand, within proV at 2393.824 kb on - strand, within proV at 2393.826 kb on + strand, within proV at 2393.830 kb on + strand, within proV at 2393.878 kb on + strand, within proV at 2394.201 kb on - strand at 2394.213 kb on + strand at 2394.221 kb on - strand at 2394.257 kb on - strand at 2394.294 kb on + strand at 2394.342 kb on + strand, within DVU2300 at 2394.618 kb on - strand at 2394.618 kb on - strand at 2394.693 kb on - strand at 2394.714 kb on + strand at 2394.794 kb on + strand at 2394.810 kb on - strand at 2394.838 kb on - strand at 2394.857 kb on + strand, within DVU2302 at 2394.860 kb on - strand, within DVU2302 at 2394.886 kb on + strand, within DVU2302 at 2394.914 kb on + strand, within DVU2302 at 2394.917 kb on - strand, within DVU2302 at 2394.923 kb on + strand, within DVU2302 at 2394.984 kb on - strand, within DVU2302 at 2395.028 kb on + strand, within DVU2302 at 2395.106 kb on - strand, within DVU2302
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine as nutrient 10mM remove 2,392,002 - -0.5 2,392,013 + +0.5 2,392,029 + +0.0 2,392,064 - +0.3 2,392,247 + opuBB DVU2298 0.12 -0.4 2,392,262 - opuBB DVU2298 0.14 +0.6 2,392,299 + opuBB DVU2298 0.18 +0.6 2,392,390 + opuBB DVU2298 0.29 +0.0 2,392,390 + opuBB DVU2298 0.29 -0.6 2,392,427 + opuBB DVU2298 0.33 -0.2 2,392,450 + opuBB DVU2298 0.36 -1.1 2,392,508 + opuBB DVU2298 0.42 -0.1 2,392,592 + opuBB DVU2298 0.52 +1.4 2,392,600 - opuBB DVU2298 0.53 -0.0 2,392,653 + opuBB DVU2298 0.60 +0.4 2,393,241 - proV DVU2299 0.21 +0.0 2,393,241 - proV DVU2299 0.21 -1.3 2,393,304 + proV DVU2299 0.26 +1.0 2,393,314 + proV DVU2299 0.27 +0.2 2,393,430 + proV DVU2299 0.37 -0.0 2,393,438 + proV DVU2299 0.37 -0.0 2,393,438 + proV DVU2299 0.37 +0.2 2,393,440 + proV DVU2299 0.37 -0.1 2,393,464 - proV DVU2299 0.39 -0.6 2,393,469 - proV DVU2299 0.40 -0.0 2,393,518 + proV DVU2299 0.44 -0.2 2,393,533 + proV DVU2299 0.45 -0.2 2,393,541 - proV DVU2299 0.46 +0.2 2,393,620 + proV DVU2299 0.52 +0.4 2,393,688 + proV DVU2299 0.58 -0.2 2,393,696 - proV DVU2299 0.59 -0.1 2,393,731 + proV DVU2299 0.62 +0.1 2,393,739 - proV DVU2299 0.62 +2.6 2,393,747 - proV DVU2299 0.63 +1.2 2,393,793 - proV DVU2299 0.67 -0.6 2,393,816 + proV DVU2299 0.69 +0.3 2,393,816 + proV DVU2299 0.69 -0.2 2,393,821 + proV DVU2299 0.69 +1.4 2,393,824 - proV DVU2299 0.70 +1.5 2,393,826 + proV DVU2299 0.70 -0.5 2,393,830 + proV DVU2299 0.70 +0.3 2,393,878 + proV DVU2299 0.74 +1.3 2,394,201 - +0.0 2,394,213 + -0.6 2,394,221 - -0.0 2,394,257 - +1.3 2,394,294 + -0.5 2,394,342 + DVU2300 0.12 +0.6 2,394,618 - -0.9 2,394,618 - +0.1 2,394,693 - +1.2 2,394,714 + -0.3 2,394,794 + +0.1 2,394,810 - -0.5 2,394,838 - -0.7 2,394,857 + DVU2302 0.11 +0.1 2,394,860 - DVU2302 0.11 +0.6 2,394,886 + DVU2302 0.12 +0.2 2,394,914 + DVU2302 0.14 -0.4 2,394,917 - DVU2302 0.14 -0.1 2,394,923 + DVU2302 0.14 -0.2 2,394,984 - DVU2302 0.17 -1.0 2,395,028 + DVU2302 0.19 -0.5 2,395,106 - DVU2302 0.23 -0.6
Or see this region's nucleotide sequence