Experiment: MoLS4 with L-Glycine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1914 and DVU1915 are separated by 41 nucleotides DVU1915 and DVU1916 overlap by 23 nucleotides DVU1916 and hysB are separated by 93 nucleotides
DVU1914: DVU1914 - (R)-citramalate synthase (EC 2.3.3.21) (from data) , at 1,988,931 to 1,990,547
DVU1914
DVU1915: DVU1915 - hypothetical protein (TIGR), at 1,990,589 to 1,990,864
DVU1915
DVU1916: DVU1916 - hypothetical protein (TIGR), at 1,990,842 to 1,990,973
DVU1916
DVU1917: hysB - periplasmic [NiFeSe] hydrogenase, small subunit (TIGR), at 1,991,067 to 1,992,020
hysB
Position (kb)
1990
1991 Strain fitness (log2 ratio)
-2
-1
0
1 at 1989.843 kb on + strand, within DVU1914 at 1989.878 kb on - strand, within DVU1914 at 1989.913 kb on + strand, within DVU1914 at 1989.981 kb on - strand, within DVU1914 at 1989.989 kb on + strand, within DVU1914 at 1990.003 kb on - strand, within DVU1914 at 1990.003 kb on - strand, within DVU1914 at 1990.135 kb on + strand, within DVU1914 at 1990.205 kb on - strand, within DVU1914 at 1990.259 kb on + strand, within DVU1914 at 1990.266 kb on + strand, within DVU1914 at 1990.283 kb on + strand, within DVU1914 at 1990.325 kb on - strand, within DVU1914 at 1990.336 kb on + strand, within DVU1914 at 1990.540 kb on - strand at 1990.947 kb on - strand, within DVU1916 at 1991.079 kb on - strand at 1991.126 kb on - strand at 1991.251 kb on + strand, within hysB at 1991.251 kb on - strand, within hysB at 1991.402 kb on - strand, within hysB at 1991.735 kb on + strand, within hysB at 1991.779 kb on + strand, within hysB at 1991.834 kb on - strand, within hysB at 1991.834 kb on - strand, within hysB at 1991.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine as nutrient 10mM remove 1,989,843 + DVU1914 0.56 -1.1 1,989,878 - DVU1914 0.59 -1.6 1,989,913 + DVU1914 0.61 -0.2 1,989,981 - DVU1914 0.65 -0.4 1,989,989 + DVU1914 0.65 +0.5 1,990,003 - DVU1914 0.66 -2.3 1,990,003 - DVU1914 0.66 -1.4 1,990,135 + DVU1914 0.74 -2.0 1,990,205 - DVU1914 0.79 -0.1 1,990,259 + DVU1914 0.82 -1.0 1,990,266 + DVU1914 0.83 -1.5 1,990,283 + DVU1914 0.84 +1.6 1,990,325 - DVU1914 0.86 -1.2 1,990,336 + DVU1914 0.87 -1.7 1,990,540 - -0.3 1,990,947 - DVU1916 0.80 +0.0 1,991,079 - +1.1 1,991,126 - +1.6 1,991,251 + hysB DVU1917 0.19 -0.7 1,991,251 - hysB DVU1917 0.19 -0.4 1,991,402 - hysB DVU1917 0.35 -0.7 1,991,735 + hysB DVU1917 0.70 +0.1 1,991,779 + hysB DVU1917 0.75 -0.6 1,991,834 - hysB DVU1917 0.80 -0.0 1,991,834 - hysB DVU1917 0.80 -0.8 1,991,929 - -0.4
Or see this region's nucleotide sequence