Experiment: MoLS4 with L-Glycine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemA and DVU1462 are separated by 1 nucleotides DVU1462 and cysG-1 are separated by 85 nucleotides cysG-1 and DVU1464 are separated by 122 nucleotides
DVU1461: hemA - glutamyl-tRNA reductase (TIGR), at 1,540,047 to 1,541,369
hemA
DVU1462: DVU1462 - cytochrome c assembly protein, putative (TIGR), at 1,541,371 to 1,542,102
DVU1462
DVU1463: cysG-1 - siroheme synthase, N-terminal domain protein (TIGR), at 1,542,188 to 1,542,865
cysG-1
DVU1464: DVU1464 - heptosyltransferase family protein (TIGR), at 1,542,988 to 1,544,223
DVU1464
Position (kb)
1542
1543 Strain fitness (log2 ratio)
-1
0
1
2 at 1541.570 kb on + strand, within DVU1462 at 1541.635 kb on + strand, within DVU1462 at 1541.635 kb on + strand, within DVU1462 at 1542.152 kb on + strand at 1542.894 kb on + strand at 1542.903 kb on - strand at 1542.957 kb on + strand at 1542.973 kb on + strand at 1542.981 kb on - strand at 1543.052 kb on + strand at 1543.052 kb on + strand at 1543.052 kb on + strand at 1543.163 kb on + strand, within DVU1464 at 1543.163 kb on + strand, within DVU1464 at 1543.163 kb on + strand, within DVU1464 at 1543.301 kb on - strand, within DVU1464 at 1543.324 kb on - strand, within DVU1464 at 1543.389 kb on - strand, within DVU1464 at 1543.416 kb on + strand, within DVU1464 at 1543.424 kb on - strand, within DVU1464 at 1543.582 kb on + strand, within DVU1464 at 1543.614 kb on + strand, within DVU1464 at 1543.664 kb on - strand, within DVU1464 at 1543.703 kb on + strand, within DVU1464 at 1543.703 kb on + strand, within DVU1464 at 1543.782 kb on + strand, within DVU1464 at 1543.794 kb on + strand, within DVU1464 at 1543.803 kb on - strand, within DVU1464 at 1543.859 kb on + strand, within DVU1464
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine as nutrient 10mM remove 1,541,570 + DVU1462 0.27 -0.2 1,541,635 + DVU1462 0.36 -1.5 1,541,635 + DVU1462 0.36 -0.3 1,542,152 + +0.2 1,542,894 + -1.6 1,542,903 - +1.3 1,542,957 + +0.2 1,542,973 + -0.8 1,542,981 - -0.1 1,543,052 + -1.0 1,543,052 + -0.1 1,543,052 + +0.3 1,543,163 + DVU1464 0.14 +2.1 1,543,163 + DVU1464 0.14 +0.2 1,543,163 + DVU1464 0.14 +0.1 1,543,301 - DVU1464 0.25 -0.4 1,543,324 - DVU1464 0.27 -0.4 1,543,389 - DVU1464 0.32 -0.7 1,543,416 + DVU1464 0.35 -1.2 1,543,424 - DVU1464 0.35 -0.3 1,543,582 + DVU1464 0.48 +0.4 1,543,614 + DVU1464 0.51 +0.4 1,543,664 - DVU1464 0.55 -0.2 1,543,703 + DVU1464 0.58 +0.0 1,543,703 + DVU1464 0.58 +0.2 1,543,782 + DVU1464 0.64 +0.2 1,543,794 + DVU1464 0.65 +0.0 1,543,803 - DVU1464 0.66 +0.2 1,543,859 + DVU1464 0.70 -1.2
Or see this region's nucleotide sequence