Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5049 and Psyr_5050 are separated by 50 nucleotides Psyr_5050 and Psyr_5051 are separated by 267 nucleotides
Psyr_5049: Psyr_5049 - conserved hypothetical protein, at 5,990,200 to 5,990,814
_5049
Psyr_5050: Psyr_5050 - transcriptional regulator, LysR family, at 5,990,865 to 5,991,812
_5050
Psyr_5051: Psyr_5051 - Aspartate ammonia-lyase, at 5,992,080 to 5,993,504
_5051
Position (kb)
5990
5991
5992 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5989.918 kb on - strand at 5989.918 kb on - strand at 5989.958 kb on - strand at 5989.958 kb on - strand at 5989.958 kb on - strand at 5989.960 kb on - strand at 5989.979 kb on - strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.066 kb on + strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.067 kb on - strand at 5990.129 kb on + strand at 5990.129 kb on + strand at 5990.129 kb on + strand at 5990.129 kb on + strand at 5990.130 kb on - strand at 5990.130 kb on - strand at 5990.130 kb on - strand at 5990.130 kb on - strand at 5990.130 kb on - strand at 5990.130 kb on - strand at 5990.166 kb on - strand at 5990.169 kb on + strand at 5990.169 kb on + strand at 5990.169 kb on + strand at 5990.196 kb on - strand at 5990.196 kb on - strand at 5990.196 kb on - strand at 5990.246 kb on - strand at 5990.310 kb on + strand, within Psyr_5049 at 5990.310 kb on + strand, within Psyr_5049 at 5990.310 kb on + strand, within Psyr_5049 at 5990.310 kb on + strand, within Psyr_5049 at 5990.310 kb on + strand, within Psyr_5049 at 5990.311 kb on - strand, within Psyr_5049 at 5990.311 kb on - strand, within Psyr_5049 at 5990.344 kb on + strand, within Psyr_5049 at 5990.344 kb on + strand, within Psyr_5049 at 5990.344 kb on + strand, within Psyr_5049 at 5990.344 kb on + strand, within Psyr_5049 at 5990.344 kb on + strand, within Psyr_5049 at 5990.345 kb on - strand, within Psyr_5049 at 5990.345 kb on - strand, within Psyr_5049 at 5990.345 kb on - strand, within Psyr_5049 at 5990.345 kb on - strand, within Psyr_5049 at 5990.345 kb on - strand, within Psyr_5049 at 5990.470 kb on + strand, within Psyr_5049 at 5990.538 kb on + strand, within Psyr_5049 at 5990.538 kb on + strand, within Psyr_5049 at 5990.539 kb on - strand, within Psyr_5049 at 5990.539 kb on - strand, within Psyr_5049 at 5990.539 kb on - strand, within Psyr_5049 at 5990.539 kb on - strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.542 kb on + strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.543 kb on - strand, within Psyr_5049 at 5990.562 kb on + strand, within Psyr_5049 at 5990.579 kb on + strand, within Psyr_5049 at 5990.604 kb on + strand, within Psyr_5049 at 5990.632 kb on + strand, within Psyr_5049 at 5990.632 kb on + strand, within Psyr_5049 at 5990.633 kb on - strand, within Psyr_5049 at 5990.633 kb on - strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.730 kb on + strand, within Psyr_5049 at 5990.731 kb on - strand, within Psyr_5049 at 5990.731 kb on - strand, within Psyr_5049 at 5990.731 kb on - strand, within Psyr_5049 at 5990.731 kb on - strand, within Psyr_5049 at 5990.749 kb on + strand, within Psyr_5049 at 5990.750 kb on - strand, within Psyr_5049 at 5990.752 kb on + strand, within Psyr_5049 at 5990.752 kb on + strand, within Psyr_5049 at 5990.752 kb on + strand, within Psyr_5049 at 5990.752 kb on + strand, within Psyr_5049 at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.753 kb on - strand at 5990.788 kb on + strand at 5990.788 kb on + strand at 5990.788 kb on + strand at 5990.788 kb on + strand at 5990.789 kb on - strand at 5990.789 kb on - strand at 5990.789 kb on - strand at 5990.832 kb on - strand at 5990.832 kb on - strand at 5990.832 kb on - strand at 5991.091 kb on + strand, within Psyr_5050 at 5991.167 kb on - strand, within Psyr_5050 at 5991.167 kb on - strand, within Psyr_5050 at 5991.167 kb on - strand, within Psyr_5050 at 5991.167 kb on - strand, within Psyr_5050 at 5991.316 kb on + strand, within Psyr_5050 at 5991.317 kb on - strand, within Psyr_5050 at 5991.317 kb on - strand, within Psyr_5050 at 5991.869 kb on + strand at 5991.870 kb on - strand at 5991.870 kb on - strand at 5991.935 kb on - strand at 5991.969 kb on - strand at 5992.135 kb on + strand at 5992.135 kb on + strand at 5992.136 kb on - strand at 5992.149 kb on + strand at 5992.149 kb on + strand at 5992.149 kb on + strand at 5992.150 kb on - strand at 5992.150 kb on - strand at 5992.153 kb on - strand at 5992.228 kb on + strand, within Psyr_5051 at 5992.228 kb on + strand, within Psyr_5051 at 5992.229 kb on - strand, within Psyr_5051
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 5,989,918 - -0.3 5,989,918 - +1.1 5,989,958 - -0.4 5,989,958 - -0.6 5,989,958 - +0.1 5,989,960 - -0.3 5,989,979 - +0.4 5,990,066 + +0.1 5,990,066 + +0.3 5,990,066 + +1.5 5,990,066 + +0.4 5,990,066 + +0.8 5,990,066 + +0.8 5,990,066 + -2.5 5,990,066 + +3.6 5,990,066 + +1.1 5,990,066 + -0.9 5,990,066 + +1.9 5,990,066 + +0.4 5,990,066 + +0.3 5,990,066 + -1.0 5,990,067 - -0.5 5,990,067 - +0.2 5,990,067 - +0.2 5,990,067 - +0.1 5,990,067 - +2.2 5,990,067 - +0.6 5,990,067 - -0.3 5,990,067 - +0.6 5,990,067 - -0.5 5,990,067 - +0.6 5,990,067 - -0.1 5,990,067 - -1.3 5,990,067 - -0.7 5,990,067 - -0.3 5,990,067 - -0.7 5,990,129 + +0.3 5,990,129 + -0.5 5,990,129 + -0.2 5,990,129 + +0.4 5,990,130 - +1.2 5,990,130 - +0.1 5,990,130 - -0.1 5,990,130 - +0.7 5,990,130 - -0.7 5,990,130 - -0.5 5,990,166 - +1.1 5,990,169 + +0.1 5,990,169 + -1.5 5,990,169 + -0.1 5,990,196 - +0.4 5,990,196 - +1.8 5,990,196 - -0.2 5,990,246 - -0.2 5,990,310 + Psyr_5049 0.18 +0.4 5,990,310 + Psyr_5049 0.18 -0.1 5,990,310 + Psyr_5049 0.18 +2.8 5,990,310 + Psyr_5049 0.18 +0.4 5,990,310 + Psyr_5049 0.18 -0.2 5,990,311 - Psyr_5049 0.18 +0.6 5,990,311 - Psyr_5049 0.18 -0.2 5,990,344 + Psyr_5049 0.23 +0.4 5,990,344 + Psyr_5049 0.23 -0.5 5,990,344 + Psyr_5049 0.23 +1.1 5,990,344 + Psyr_5049 0.23 -1.3 5,990,344 + Psyr_5049 0.23 -0.6 5,990,345 - Psyr_5049 0.24 +2.4 5,990,345 - Psyr_5049 0.24 +0.8 5,990,345 - Psyr_5049 0.24 +0.7 5,990,345 - Psyr_5049 0.24 -0.0 5,990,345 - Psyr_5049 0.24 +0.4 5,990,470 + Psyr_5049 0.44 +0.2 5,990,538 + Psyr_5049 0.55 -0.1 5,990,538 + Psyr_5049 0.55 -0.1 5,990,539 - Psyr_5049 0.55 -0.5 5,990,539 - Psyr_5049 0.55 +0.2 5,990,539 - Psyr_5049 0.55 -0.4 5,990,539 - Psyr_5049 0.55 +0.0 5,990,542 + Psyr_5049 0.56 +0.9 5,990,542 + Psyr_5049 0.56 +2.2 5,990,542 + Psyr_5049 0.56 +1.2 5,990,542 + Psyr_5049 0.56 +0.2 5,990,542 + Psyr_5049 0.56 +0.9 5,990,542 + Psyr_5049 0.56 -0.2 5,990,542 + Psyr_5049 0.56 +0.2 5,990,542 + Psyr_5049 0.56 +1.0 5,990,543 - Psyr_5049 0.56 +1.3 5,990,543 - Psyr_5049 0.56 -0.1 5,990,543 - Psyr_5049 0.56 -0.5 5,990,543 - Psyr_5049 0.56 +1.5 5,990,543 - Psyr_5049 0.56 +0.4 5,990,543 - Psyr_5049 0.56 -0.8 5,990,543 - Psyr_5049 0.56 -0.5 5,990,543 - Psyr_5049 0.56 -1.2 5,990,543 - Psyr_5049 0.56 +0.2 5,990,543 - Psyr_5049 0.56 +0.8 5,990,543 - Psyr_5049 0.56 +0.4 5,990,543 - Psyr_5049 0.56 +0.9 5,990,562 + Psyr_5049 0.59 -0.8 5,990,579 + Psyr_5049 0.62 +0.6 5,990,604 + Psyr_5049 0.66 +0.7 5,990,632 + Psyr_5049 0.70 +0.1 5,990,632 + Psyr_5049 0.70 -0.2 5,990,633 - Psyr_5049 0.70 -1.2 5,990,633 - Psyr_5049 0.70 +0.2 5,990,730 + Psyr_5049 0.86 -1.7 5,990,730 + Psyr_5049 0.86 +0.1 5,990,730 + Psyr_5049 0.86 +0.5 5,990,730 + Psyr_5049 0.86 -0.0 5,990,730 + Psyr_5049 0.86 +1.9 5,990,730 + Psyr_5049 0.86 -0.8 5,990,731 - Psyr_5049 0.86 +0.1 5,990,731 - Psyr_5049 0.86 +1.1 5,990,731 - Psyr_5049 0.86 +0.5 5,990,731 - Psyr_5049 0.86 +1.0 5,990,749 + Psyr_5049 0.89 -0.1 5,990,750 - Psyr_5049 0.89 +0.3 5,990,752 + Psyr_5049 0.90 -0.3 5,990,752 + Psyr_5049 0.90 +0.1 5,990,752 + Psyr_5049 0.90 +0.2 5,990,752 + Psyr_5049 0.90 -0.7 5,990,753 - -0.3 5,990,753 - +0.3 5,990,753 - -0.2 5,990,753 - +0.2 5,990,753 - +0.1 5,990,753 - +0.5 5,990,753 - +0.2 5,990,788 + +0.5 5,990,788 + +0.1 5,990,788 + -0.6 5,990,788 + +0.5 5,990,789 - +0.1 5,990,789 - +0.0 5,990,789 - +1.1 5,990,832 - -0.3 5,990,832 - +0.5 5,990,832 - -0.9 5,991,091 + Psyr_5050 0.24 -0.0 5,991,167 - Psyr_5050 0.32 -1.3 5,991,167 - Psyr_5050 0.32 -0.5 5,991,167 - Psyr_5050 0.32 -2.1 5,991,167 - Psyr_5050 0.32 -1.5 5,991,316 + Psyr_5050 0.48 +1.8 5,991,317 - Psyr_5050 0.48 -0.5 5,991,317 - Psyr_5050 0.48 -1.8 5,991,869 + +0.5 5,991,870 - -0.5 5,991,870 - -0.9 5,991,935 - -1.0 5,991,969 - +0.1 5,992,135 + +0.5 5,992,135 + +0.2 5,992,136 - -1.1 5,992,149 + -1.1 5,992,149 + -0.4 5,992,149 + +0.7 5,992,150 - +0.2 5,992,150 - +0.5 5,992,153 - -0.1 5,992,228 + Psyr_5051 0.10 +0.9 5,992,228 + Psyr_5051 0.10 -0.1 5,992,229 - Psyr_5051 0.10 +0.1
Or see this region's nucleotide sequence