Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4462 and Psyr_4463 are separated by 41 nucleotides Psyr_4463 and Psyr_4464 are separated by 143 nucleotides Psyr_4464 and Psyr_4465 overlap by 4 nucleotides Psyr_4465 and Psyr_4466 are separated by 269 nucleotides
Psyr_4462: Psyr_4462 - diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase, at 5,324,768 to 5,325,868
_4462
Psyr_4463: Psyr_4463 - Protein of unknown function DUF193, at 5,325,910 to 5,326,407
_4463
Psyr_4464: Psyr_4464 - lipoprotein, putative, at 5,326,551 to 5,327,000
_4464
Psyr_4465: Psyr_4465 - conserved domain protein, at 5,326,997 to 5,327,650
_4465
Psyr_4466: Psyr_4466 - Thioredoxin, at 5,327,920 to 5,328,792
_4466
Position (kb) 5326
5327
5328 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.871 kb on - strand at 5325.912 kb on - strand at 5325.912 kb on - strand at 5325.912 kb on - strand at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.005 kb on - strand, within Psyr_4463 at 5326.301 kb on - strand, within Psyr_4463 at 5326.301 kb on - strand, within Psyr_4463 at 5326.450 kb on + strand at 5326.450 kb on + strand at 5326.450 kb on + strand at 5326.451 kb on - strand at 5326.451 kb on - strand at 5326.451 kb on - strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.452 kb on + strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.453 kb on - strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.454 kb on + strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.455 kb on - strand at 5326.465 kb on + strand at 5326.465 kb on + strand at 5326.497 kb on + strand at 5326.497 kb on + strand at 5326.498 kb on - strand at 5326.498 kb on - strand at 5326.498 kb on - strand at 5326.559 kb on - strand at 5326.574 kb on - strand at 5326.693 kb on - strand, within Psyr_4464 at 5327.169 kb on - strand, within Psyr_4465 at 5327.393 kb on + strand, within Psyr_4465 at 5327.659 kb on + strand at 5327.659 kb on + strand at 5327.659 kb on + strand at 5327.659 kb on + strand at 5327.660 kb on - strand at 5327.660 kb on - strand at 5327.660 kb on - strand at 5327.660 kb on - strand at 5327.660 kb on - strand at 5327.682 kb on - strand at 5327.694 kb on + strand at 5327.695 kb on - strand at 5327.817 kb on + strand at 5327.817 kb on + strand at 5327.818 kb on - strand at 5327.818 kb on - strand at 5327.848 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.886 kb on + strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.887 kb on - strand at 5327.938 kb on + strand at 5327.938 kb on + strand at 5327.938 kb on + strand at 5327.938 kb on + strand at 5327.939 kb on - strand at 5327.939 kb on - strand at 5327.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 5,325,871 - -0.5 5,325,871 - -0.3 5,325,871 - -0.5 5,325,871 - +1.5 5,325,912 - +1.6 5,325,912 - -0.7 5,325,912 - +0.4 5,326,005 - Psyr_4463 0.19 +0.9 5,326,005 - Psyr_4463 0.19 +1.0 5,326,005 - Psyr_4463 0.19 +2.4 5,326,005 - Psyr_4463 0.19 +0.4 5,326,005 - Psyr_4463 0.19 -0.0 5,326,005 - Psyr_4463 0.19 -1.9 5,326,301 - Psyr_4463 0.79 -0.3 5,326,301 - Psyr_4463 0.79 +0.1 5,326,450 + +2.2 5,326,450 + +0.6 5,326,450 + +0.3 5,326,451 - -0.5 5,326,451 - -1.2 5,326,451 - -1.6 5,326,452 + +0.6 5,326,452 + +0.4 5,326,452 + +0.4 5,326,452 + -1.0 5,326,452 + +1.0 5,326,452 + -0.1 5,326,452 + -1.8 5,326,452 + +0.5 5,326,453 - -0.3 5,326,453 - -0.2 5,326,453 - -0.1 5,326,453 - -1.8 5,326,453 - -1.0 5,326,453 - -0.2 5,326,454 + -0.4 5,326,454 + -0.7 5,326,454 + -1.7 5,326,454 + +2.3 5,326,454 + -0.4 5,326,454 + -0.5 5,326,454 + -0.8 5,326,454 + +1.1 5,326,454 + +1.3 5,326,455 - +1.0 5,326,455 - -1.1 5,326,455 - +1.3 5,326,455 - +1.0 5,326,455 - +0.1 5,326,455 - +0.5 5,326,455 - +0.4 5,326,465 + +0.1 5,326,465 + -0.8 5,326,497 + -0.8 5,326,497 + -0.8 5,326,498 - -0.5 5,326,498 - -0.7 5,326,498 - +0.2 5,326,559 - -0.3 5,326,574 - +0.2 5,326,693 - Psyr_4464 0.32 -0.9 5,327,169 - Psyr_4465 0.26 -0.3 5,327,393 + Psyr_4465 0.61 -0.6 5,327,659 + -2.2 5,327,659 + -0.6 5,327,659 + +1.2 5,327,659 + +0.6 5,327,660 - -0.5 5,327,660 - -1.2 5,327,660 - -0.3 5,327,660 - +0.0 5,327,660 - -0.0 5,327,682 - -0.2 5,327,694 + +0.2 5,327,695 - +0.8 5,327,817 + +1.0 5,327,817 + -0.2 5,327,818 - +0.7 5,327,818 - -1.8 5,327,848 + +0.6 5,327,886 + +1.0 5,327,886 + +1.0 5,327,886 + +2.6 5,327,886 + -0.3 5,327,886 + +0.5 5,327,886 + +0.8 5,327,886 + -1.8 5,327,887 - -1.1 5,327,887 - +1.0 5,327,887 - +1.1 5,327,887 - +0.2 5,327,887 - -0.0 5,327,887 - -1.1 5,327,887 - -0.6 5,327,938 + -0.5 5,327,938 + +0.2 5,327,938 + -0.6 5,327,938 + +2.4 5,327,939 - -1.1 5,327,939 - +0.7 5,327,939 - +0.2
Or see this region's nucleotide sequence