Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3581 and Psyr_3582 are separated by 184 nucleotides Psyr_3582 and Psyr_3583 are separated by 304 nucleotides
Psyr_3581: Psyr_3581 - ribosomal large subunit pseudouridine synthase B, at 4,264,625 to 4,265,854
_3581
Psyr_3582: Psyr_3582 - Protein of unknown function DUF1289, at 4,266,039 to 4,266,230
_3582
Psyr_3583: Psyr_3583 - condensin subunit ScpB, at 4,266,535 to 4,267,302
_3583
Position (kb)
4266
4267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4265.228 kb on + strand, within Psyr_3581 at 4265.228 kb on + strand, within Psyr_3581 at 4265.228 kb on + strand, within Psyr_3581 at 4265.229 kb on - strand, within Psyr_3581 at 4265.229 kb on - strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.604 kb on + strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.605 kb on - strand, within Psyr_3581 at 4265.944 kb on - strand at 4265.944 kb on - strand at 4265.944 kb on - strand at 4265.944 kb on - strand at 4265.944 kb on - strand at 4266.355 kb on + strand at 4266.355 kb on + strand at 4266.356 kb on - strand at 4266.383 kb on + strand at 4266.383 kb on + strand at 4266.384 kb on - strand at 4266.384 kb on - strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.387 kb on + strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.388 kb on - strand at 4266.444 kb on - strand at 4266.498 kb on - strand at 4266.498 kb on - strand at 4266.498 kb on - strand at 4266.529 kb on - strand at 4267.214 kb on + strand, within Psyr_3583 at 4267.214 kb on + strand, within Psyr_3583 at 4267.214 kb on + strand, within Psyr_3583 at 4267.214 kb on + strand, within Psyr_3583 at 4267.214 kb on + strand, within Psyr_3583 at 4267.215 kb on - strand, within Psyr_3583 at 4267.215 kb on - strand, within Psyr_3583
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 4,265,228 + Psyr_3581 0.49 -1.0 4,265,228 + Psyr_3581 0.49 -0.0 4,265,228 + Psyr_3581 0.49 -1.3 4,265,229 - Psyr_3581 0.49 -0.2 4,265,229 - Psyr_3581 0.49 -2.1 4,265,604 + Psyr_3581 0.80 -0.9 4,265,604 + Psyr_3581 0.80 -1.4 4,265,604 + Psyr_3581 0.80 -0.7 4,265,604 + Psyr_3581 0.80 -2.3 4,265,604 + Psyr_3581 0.80 -1.4 4,265,604 + Psyr_3581 0.80 -0.2 4,265,604 + Psyr_3581 0.80 -0.6 4,265,604 + Psyr_3581 0.80 -2.0 4,265,604 + Psyr_3581 0.80 -1.9 4,265,605 - Psyr_3581 0.80 -2.9 4,265,605 - Psyr_3581 0.80 -1.7 4,265,605 - Psyr_3581 0.80 -1.3 4,265,605 - Psyr_3581 0.80 -1.2 4,265,605 - Psyr_3581 0.80 -0.7 4,265,605 - Psyr_3581 0.80 -0.7 4,265,605 - Psyr_3581 0.80 -3.1 4,265,605 - Psyr_3581 0.80 -1.2 4,265,605 - Psyr_3581 0.80 -0.3 4,265,944 - +0.8 4,265,944 - +1.3 4,265,944 - -0.5 4,265,944 - +0.8 4,265,944 - +0.8 4,266,355 + +1.1 4,266,355 + +0.1 4,266,356 - +1.3 4,266,383 + +0.8 4,266,383 + +0.1 4,266,384 - +0.6 4,266,384 - +0.8 4,266,387 + +0.6 4,266,387 + -2.2 4,266,387 + -1.6 4,266,387 + -0.2 4,266,387 + -1.5 4,266,387 + -0.7 4,266,387 + +0.5 4,266,387 + -0.2 4,266,387 + -0.6 4,266,387 + +1.7 4,266,388 - +1.2 4,266,388 - +0.3 4,266,388 - +0.5 4,266,388 - +0.5 4,266,388 - +0.8 4,266,388 - +0.1 4,266,388 - -0.2 4,266,388 - +1.3 4,266,388 - +1.6 4,266,388 - +0.6 4,266,388 - +1.0 4,266,388 - +0.7 4,266,388 - -0.2 4,266,388 - +0.3 4,266,388 - -1.1 4,266,388 - +0.1 4,266,388 - +0.6 4,266,388 - +0.1 4,266,388 - +1.2 4,266,388 - +0.4 4,266,388 - -0.0 4,266,444 - +0.4 4,266,498 - +0.6 4,266,498 - -0.5 4,266,498 - +0.4 4,266,529 - +1.8 4,267,214 + Psyr_3583 0.88 +0.8 4,267,214 + Psyr_3583 0.88 +0.7 4,267,214 + Psyr_3583 0.88 -0.8 4,267,214 + Psyr_3583 0.88 +0.3 4,267,214 + Psyr_3583 0.88 +1.3 4,267,215 - Psyr_3583 0.89 +1.0 4,267,215 - Psyr_3583 0.89 +4.0
Or see this region's nucleotide sequence