Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3353 and Psyr_3354 are separated by 61 nucleotides Psyr_3354 and Psyr_3355 are separated by 12 nucleotides Psyr_3355 and Psyr_3356 are separated by 369 nucleotides Psyr_3356 and Psyr_3357 are separated by 109 nucleotides
Psyr_3353: Psyr_3353 - Catalase, at 4,010,342 to 4,011,874
_3353
Psyr_3354: Psyr_3354 - Ankyrin, at 4,011,936 to 4,012,505
_3354
Psyr_3355: Psyr_3355 - PAS, at 4,012,518 to 4,014,458
_3355
Psyr_3356: Psyr_3356 - conserved hypothetical protein, at 4,014,828 to 4,015,052
_3356
Psyr_3357: Psyr_3357 - transcriptional regulator, LysR family, at 4,015,162 to 4,016,046
_3357
Position (kb)
4012
4013
4014
4015 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4011.545 kb on + strand, within Psyr_3353 at 4011.545 kb on + strand, within Psyr_3353 at 4011.546 kb on - strand, within Psyr_3353 at 4011.546 kb on - strand, within Psyr_3353 at 4011.774 kb on - strand at 4011.779 kb on + strand at 4011.779 kb on + strand at 4011.779 kb on + strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.797 kb on + strand at 4011.797 kb on + strand at 4011.951 kb on + strand at 4012.125 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.156 kb on - strand, within Psyr_3354 at 4012.156 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.369 kb on - strand, within Psyr_3354 at 4012.369 kb on - strand, within Psyr_3354 at 4012.543 kb on + strand at 4012.813 kb on + strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.951 kb on + strand, within Psyr_3355 at 4012.952 kb on - strand, within Psyr_3355 at 4012.966 kb on + strand, within Psyr_3355 at 4012.966 kb on + strand, within Psyr_3355 at 4013.002 kb on + strand, within Psyr_3355 at 4013.003 kb on - strand, within Psyr_3355 at 4013.003 kb on - strand, within Psyr_3355 at 4013.042 kb on - strand, within Psyr_3355 at 4013.167 kb on + strand, within Psyr_3355 at 4013.167 kb on + strand, within Psyr_3355 at 4013.168 kb on - strand, within Psyr_3355 at 4013.517 kb on + strand, within Psyr_3355 at 4013.725 kb on + strand, within Psyr_3355 at 4013.725 kb on + strand, within Psyr_3355 at 4013.737 kb on + strand, within Psyr_3355 at 4013.737 kb on + strand, within Psyr_3355 at 4013.779 kb on + strand, within Psyr_3355 at 4013.779 kb on + strand, within Psyr_3355 at 4013.784 kb on + strand, within Psyr_3355 at 4013.785 kb on - strand, within Psyr_3355 at 4013.785 kb on - strand, within Psyr_3355 at 4013.921 kb on + strand, within Psyr_3355 at 4013.921 kb on + strand, within Psyr_3355 at 4013.921 kb on + strand, within Psyr_3355 at 4013.921 kb on + strand, within Psyr_3355 at 4013.922 kb on - strand, within Psyr_3355 at 4013.922 kb on - strand, within Psyr_3355 at 4014.163 kb on - strand, within Psyr_3355 at 4014.163 kb on - strand, within Psyr_3355 at 4014.218 kb on - strand, within Psyr_3355 at 4014.364 kb on + strand at 4014.364 kb on + strand at 4014.365 kb on - strand at 4014.365 kb on - strand at 4014.365 kb on - strand at 4014.365 kb on - strand at 4014.370 kb on + strand at 4014.371 kb on - strand at 4014.397 kb on + strand at 4014.397 kb on + strand at 4014.397 kb on + strand at 4014.398 kb on - strand at 4014.398 kb on - strand at 4014.594 kb on + strand at 4014.595 kb on - strand at 4014.595 kb on - strand at 4014.595 kb on - strand at 4014.676 kb on - strand at 4014.723 kb on + strand at 4014.724 kb on - strand at 4014.995 kb on - strand at 4015.090 kb on + strand at 4015.090 kb on + strand at 4015.091 kb on - strand at 4015.092 kb on + strand at 4015.376 kb on + strand, within Psyr_3357 at 4015.376 kb on + strand, within Psyr_3357 at 4015.376 kb on + strand, within Psyr_3357 at 4015.377 kb on - strand, within Psyr_3357 at 4015.450 kb on - strand, within Psyr_3357 at 4015.450 kb on - strand, within Psyr_3357
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 4,011,545 + Psyr_3353 0.78 -0.4 4,011,545 + Psyr_3353 0.78 -0.6 4,011,546 - Psyr_3353 0.79 -0.4 4,011,546 - Psyr_3353 0.79 +1.9 4,011,774 - +0.5 4,011,779 + +0.4 4,011,779 + +0.5 4,011,779 + -1.1 4,011,780 - -1.5 4,011,780 - -1.7 4,011,780 - +1.3 4,011,780 - +0.3 4,011,797 + +0.0 4,011,797 + +0.0 4,011,951 + +1.1 4,012,125 + Psyr_3354 0.33 -0.1 4,012,155 + Psyr_3354 0.38 +0.4 4,012,155 + Psyr_3354 0.38 -0.8 4,012,155 + Psyr_3354 0.38 -0.1 4,012,155 + Psyr_3354 0.38 +0.9 4,012,156 - Psyr_3354 0.39 -0.3 4,012,156 - Psyr_3354 0.39 -0.5 4,012,205 - Psyr_3354 0.47 -0.7 4,012,205 - Psyr_3354 0.47 -0.3 4,012,205 - Psyr_3354 0.47 -1.0 4,012,368 + Psyr_3354 0.76 -0.5 4,012,368 + Psyr_3354 0.76 +0.4 4,012,368 + Psyr_3354 0.76 -0.4 4,012,369 - Psyr_3354 0.76 +1.3 4,012,369 - Psyr_3354 0.76 -0.6 4,012,543 + +0.3 4,012,813 + Psyr_3355 0.15 -0.3 4,012,814 - Psyr_3355 0.15 +0.7 4,012,814 - Psyr_3355 0.15 -0.9 4,012,814 - Psyr_3355 0.15 +0.0 4,012,814 - Psyr_3355 0.15 -1.7 4,012,951 + Psyr_3355 0.22 -0.7 4,012,952 - Psyr_3355 0.22 -0.8 4,012,966 + Psyr_3355 0.23 -1.3 4,012,966 + Psyr_3355 0.23 +0.9 4,013,002 + Psyr_3355 0.25 +1.0 4,013,003 - Psyr_3355 0.25 +0.2 4,013,003 - Psyr_3355 0.25 -0.6 4,013,042 - Psyr_3355 0.27 -0.1 4,013,167 + Psyr_3355 0.33 +0.3 4,013,167 + Psyr_3355 0.33 -1.2 4,013,168 - Psyr_3355 0.33 +1.5 4,013,517 + Psyr_3355 0.51 -2.8 4,013,725 + Psyr_3355 0.62 +0.9 4,013,725 + Psyr_3355 0.62 -1.1 4,013,737 + Psyr_3355 0.63 -0.1 4,013,737 + Psyr_3355 0.63 -1.4 4,013,779 + Psyr_3355 0.65 +0.4 4,013,779 + Psyr_3355 0.65 -0.7 4,013,784 + Psyr_3355 0.65 -1.9 4,013,785 - Psyr_3355 0.65 -0.7 4,013,785 - Psyr_3355 0.65 +0.1 4,013,921 + Psyr_3355 0.72 -3.5 4,013,921 + Psyr_3355 0.72 +2.1 4,013,921 + Psyr_3355 0.72 -0.3 4,013,921 + Psyr_3355 0.72 +2.0 4,013,922 - Psyr_3355 0.72 -1.4 4,013,922 - Psyr_3355 0.72 +0.4 4,014,163 - Psyr_3355 0.85 -0.5 4,014,163 - Psyr_3355 0.85 -0.8 4,014,218 - Psyr_3355 0.88 +0.5 4,014,364 + -1.3 4,014,364 + -1.5 4,014,365 - +0.2 4,014,365 - -0.3 4,014,365 - +0.0 4,014,365 - +0.3 4,014,370 + +1.0 4,014,371 - +0.4 4,014,397 + +0.1 4,014,397 + +0.9 4,014,397 + +0.4 4,014,398 - -0.4 4,014,398 - -0.5 4,014,594 + -0.7 4,014,595 - +0.2 4,014,595 - +3.2 4,014,595 - +0.9 4,014,676 - +0.5 4,014,723 + +2.6 4,014,724 - +0.3 4,014,995 - -2.3 4,015,090 + -0.1 4,015,090 + -1.0 4,015,091 - -0.5 4,015,092 + -0.3 4,015,376 + Psyr_3357 0.24 -0.3 4,015,376 + Psyr_3357 0.24 +1.6 4,015,376 + Psyr_3357 0.24 +2.4 4,015,377 - Psyr_3357 0.24 -0.8 4,015,450 - Psyr_3357 0.33 -1.5 4,015,450 - Psyr_3357 0.33 -0.9
Or see this region's nucleotide sequence