Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3287 and Psyr_3288 are separated by 113 nucleotides Psyr_3288 and Psyr_3289 are separated by 102 nucleotides Psyr_3289 and Psyr_3290 are separated by 4 nucleotides
Psyr_3287: Psyr_3287 - DNA topoisomerase I, at 3,932,531 to 3,935,143
_3287
Psyr_3288: Psyr_3288 - conserved hypothetical protein, at 3,935,257 to 3,935,493
_3288
Psyr_3289: Psyr_3289 - 3-ketoacyl-CoA thiolase, at 3,935,596 to 3,936,771
_3289
Psyr_3290: Psyr_3290 - 3-hydroxyacyl-CoA dehydrogenase / short chain enoyl-CoA hydratase, at 3,936,776 to 3,938,941
_3290
Position (kb)
3935
3936
3937 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3935.167 kb on - strand at 3935.192 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.204 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.206 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.208 kb on - strand at 3935.210 kb on - strand at 3935.210 kb on - strand at 3935.210 kb on - strand at 3935.210 kb on - strand at 3935.447 kb on + strand, within Psyr_3288 at 3935.462 kb on + strand, within Psyr_3288 at 3935.462 kb on + strand, within Psyr_3288 at 3935.462 kb on + strand, within Psyr_3288 at 3935.463 kb on - strand, within Psyr_3288 at 3935.471 kb on + strand at 3935.509 kb on + strand at 3935.509 kb on + strand at 3935.510 kb on - strand at 3935.510 kb on - strand at 3935.640 kb on + strand at 3935.640 kb on + strand at 3935.640 kb on + strand at 3935.640 kb on + strand at 3935.640 kb on + strand at 3935.865 kb on + strand, within Psyr_3289 at 3935.866 kb on - strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.762 kb on + strand at 3936.852 kb on + strand at 3936.852 kb on + strand at 3936.853 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3937.093 kb on - strand, within Psyr_3290 at 3937.173 kb on + strand, within Psyr_3290 at 3937.174 kb on - strand, within Psyr_3290 at 3937.423 kb on - strand, within Psyr_3290
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 3,935,167 - +2.1 3,935,192 - +1.0 3,935,204 - +0.6 3,935,204 - +0.1 3,935,204 - -0.4 3,935,204 - +0.3 3,935,204 - +0.3 3,935,204 - -1.5 3,935,204 - +1.2 3,935,206 - +0.7 3,935,206 - +2.0 3,935,206 - +0.4 3,935,206 - -0.1 3,935,206 - -1.0 3,935,206 - +0.5 3,935,206 - +0.8 3,935,206 - -0.9 3,935,206 - +1.1 3,935,206 - +1.3 3,935,206 - +0.0 3,935,206 - -0.1 3,935,208 - -0.7 3,935,208 - +2.5 3,935,208 - +0.6 3,935,208 - -1.2 3,935,208 - -0.3 3,935,208 - +0.5 3,935,208 - -1.5 3,935,208 - -0.5 3,935,208 - +0.1 3,935,208 - +0.9 3,935,208 - +2.2 3,935,208 - +0.9 3,935,208 - -0.8 3,935,208 - -0.7 3,935,208 - -0.6 3,935,208 - +0.2 3,935,208 - +0.3 3,935,208 - +0.6 3,935,208 - -1.2 3,935,208 - +0.1 3,935,210 - -0.7 3,935,210 - +1.6 3,935,210 - -1.4 3,935,210 - -0.9 3,935,447 + Psyr_3288 0.80 +0.6 3,935,462 + Psyr_3288 0.86 -0.2 3,935,462 + Psyr_3288 0.86 -0.4 3,935,462 + Psyr_3288 0.86 +1.7 3,935,463 - Psyr_3288 0.87 +0.9 3,935,471 + -0.4 3,935,509 + +0.0 3,935,509 + +1.9 3,935,510 - -0.3 3,935,510 - +0.9 3,935,640 + -1.2 3,935,640 + -0.1 3,935,640 + -0.5 3,935,640 + +0.9 3,935,640 + -0.3 3,935,865 + Psyr_3289 0.23 -1.3 3,935,866 - Psyr_3289 0.23 -2.1 3,936,061 + Psyr_3289 0.40 -0.3 3,936,061 + Psyr_3289 0.40 -1.1 3,936,061 + Psyr_3289 0.40 -1.7 3,936,672 + +0.2 3,936,672 + -1.3 3,936,672 + -0.4 3,936,672 + +0.9 3,936,672 + -2.0 3,936,672 + +0.9 3,936,673 - -1.1 3,936,673 - -1.4 3,936,673 - +2.5 3,936,673 - -1.5 3,936,762 + -1.3 3,936,852 + -0.1 3,936,852 + -0.4 3,936,853 - -1.3 3,936,964 - +0.9 3,936,964 - -1.5 3,936,964 - +0.9 3,936,964 - -3.0 3,937,093 - Psyr_3290 0.15 -3.5 3,937,173 + Psyr_3290 0.18 +0.9 3,937,174 - Psyr_3290 0.18 +0.1 3,937,423 - Psyr_3290 0.30 -2.6
Or see this region's nucleotide sequence