Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3225 and Psyr_3226 are separated by 52 nucleotides Psyr_3226 and Psyr_3227 are separated by 85 nucleotides Psyr_3227 and Psyr_3228 overlap by 4 nucleotides
Psyr_3225: Psyr_3225 - Protein-tyrosine kinase, at 3,862,476 to 3,864,683
_3225
Psyr_3226: Psyr_3226 - Transcriptional activator RfaH, at 3,864,736 to 3,865,248
_3226
Psyr_3227: Psyr_3227 - Glycosyl transferase, family 2, at 3,865,334 to 3,866,335
_3227
Psyr_3228: Psyr_3228 - Glycosyl transferase, group 1, at 3,866,332 to 3,867,537
_3228
Position (kb)
3865
3866
3867 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3864.423 kb on + strand, within Psyr_3225 at 3864.424 kb on - strand, within Psyr_3225 at 3864.424 kb on - strand, within Psyr_3225 at 3864.424 kb on - strand, within Psyr_3225 at 3864.637 kb on - strand at 3864.657 kb on + strand at 3864.663 kb on + strand at 3864.663 kb on + strand at 3864.664 kb on - strand at 3864.664 kb on - strand at 3864.664 kb on - strand at 3864.681 kb on + strand at 3864.721 kb on + strand at 3864.722 kb on - strand at 3864.722 kb on - strand at 3864.731 kb on + strand at 3864.731 kb on + strand at 3864.732 kb on - strand at 3864.911 kb on - strand, within Psyr_3226 at 3865.179 kb on + strand, within Psyr_3226 at 3865.179 kb on + strand, within Psyr_3226 at 3865.179 kb on + strand, within Psyr_3226 at 3865.179 kb on + strand, within Psyr_3226 at 3865.180 kb on - strand, within Psyr_3226 at 3865.180 kb on - strand, within Psyr_3226 at 3865.180 kb on - strand, within Psyr_3226 at 3865.308 kb on + strand at 3865.309 kb on - strand at 3865.309 kb on - strand at 3865.309 kb on - strand at 3865.309 kb on - strand at 3865.309 kb on - strand at 3865.367 kb on + strand at 3865.367 kb on + strand at 3865.367 kb on + strand at 3865.367 kb on + strand at 3865.367 kb on + strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.368 kb on - strand at 3865.385 kb on + strand at 3865.385 kb on + strand at 3865.392 kb on - strand at 3865.555 kb on - strand, within Psyr_3227 at 3865.594 kb on - strand, within Psyr_3227 at 3865.594 kb on - strand, within Psyr_3227 at 3865.641 kb on + strand, within Psyr_3227 at 3865.641 kb on + strand, within Psyr_3227 at 3865.962 kb on - strand, within Psyr_3227 at 3865.962 kb on - strand, within Psyr_3227 at 3865.962 kb on - strand, within Psyr_3227 at 3865.962 kb on - strand, within Psyr_3227 at 3865.980 kb on - strand, within Psyr_3227 at 3866.088 kb on - strand, within Psyr_3227 at 3866.088 kb on - strand, within Psyr_3227 at 3866.097 kb on - strand, within Psyr_3227 at 3866.126 kb on + strand, within Psyr_3227 at 3866.127 kb on - strand, within Psyr_3227 at 3866.231 kb on + strand, within Psyr_3227 at 3866.231 kb on + strand, within Psyr_3227 at 3866.232 kb on - strand, within Psyr_3227 at 3866.359 kb on + strand at 3866.404 kb on - strand at 3866.593 kb on + strand, within Psyr_3228 at 3866.593 kb on + strand, within Psyr_3228 at 3866.614 kb on + strand, within Psyr_3228 at 3866.746 kb on + strand, within Psyr_3228 at 3866.746 kb on + strand, within Psyr_3228 at 3866.912 kb on - strand, within Psyr_3228 at 3866.912 kb on - strand, within Psyr_3228 at 3866.932 kb on + strand, within Psyr_3228 at 3866.933 kb on - strand, within Psyr_3228 at 3866.933 kb on - strand, within Psyr_3228 at 3867.019 kb on + strand, within Psyr_3228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 3,864,423 + Psyr_3225 0.88 +0.9 3,864,424 - Psyr_3225 0.88 +0.0 3,864,424 - Psyr_3225 0.88 +1.6 3,864,424 - Psyr_3225 0.88 +0.7 3,864,637 - -1.8 3,864,657 + +0.4 3,864,663 + +0.1 3,864,663 + +0.6 3,864,664 - +0.2 3,864,664 - -0.1 3,864,664 - +0.9 3,864,681 + -1.0 3,864,721 + -1.5 3,864,722 - +0.3 3,864,722 - -0.9 3,864,731 + +1.4 3,864,731 + -0.7 3,864,732 - +0.4 3,864,911 - Psyr_3226 0.34 +1.2 3,865,179 + Psyr_3226 0.86 +1.6 3,865,179 + Psyr_3226 0.86 +0.0 3,865,179 + Psyr_3226 0.86 -1.2 3,865,179 + Psyr_3226 0.86 +0.1 3,865,180 - Psyr_3226 0.87 -0.1 3,865,180 - Psyr_3226 0.87 +0.5 3,865,180 - Psyr_3226 0.87 +0.5 3,865,308 + -0.1 3,865,309 - +0.1 3,865,309 - +3.4 3,865,309 - +0.1 3,865,309 - -0.4 3,865,309 - +0.3 3,865,367 + -2.0 3,865,367 + -0.7 3,865,367 + -0.5 3,865,367 + -2.3 3,865,367 + +2.2 3,865,368 - +0.2 3,865,368 - -1.8 3,865,368 - -0.3 3,865,368 - +0.3 3,865,368 - +0.1 3,865,368 - +0.5 3,865,368 - +0.8 3,865,385 + +0.9 3,865,385 + -0.1 3,865,392 - -1.7 3,865,555 - Psyr_3227 0.22 +1.4 3,865,594 - Psyr_3227 0.26 +0.9 3,865,594 - Psyr_3227 0.26 -0.6 3,865,641 + Psyr_3227 0.31 -0.6 3,865,641 + Psyr_3227 0.31 -2.3 3,865,962 - Psyr_3227 0.63 +0.8 3,865,962 - Psyr_3227 0.63 -0.1 3,865,962 - Psyr_3227 0.63 +1.4 3,865,962 - Psyr_3227 0.63 -0.1 3,865,980 - Psyr_3227 0.64 +1.0 3,866,088 - Psyr_3227 0.75 +0.4 3,866,088 - Psyr_3227 0.75 +1.2 3,866,097 - Psyr_3227 0.76 +0.3 3,866,126 + Psyr_3227 0.79 +0.3 3,866,127 - Psyr_3227 0.79 +0.1 3,866,231 + Psyr_3227 0.90 +0.9 3,866,231 + Psyr_3227 0.90 +1.0 3,866,232 - Psyr_3227 0.90 +0.6 3,866,359 + -1.1 3,866,404 - +0.3 3,866,593 + Psyr_3228 0.22 +0.6 3,866,593 + Psyr_3228 0.22 +1.3 3,866,614 + Psyr_3228 0.23 +2.1 3,866,746 + Psyr_3228 0.34 +0.6 3,866,746 + Psyr_3228 0.34 +2.4 3,866,912 - Psyr_3228 0.48 -0.9 3,866,912 - Psyr_3228 0.48 -1.9 3,866,932 + Psyr_3228 0.50 +0.6 3,866,933 - Psyr_3228 0.50 -1.2 3,866,933 - Psyr_3228 0.50 +0.9 3,867,019 + Psyr_3228 0.57 +0.7
Or see this region's nucleotide sequence