Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3017 and Psyr_3018 are separated by 10 nucleotides Psyr_3018 and Psyr_3019 overlap by 23 nucleotides Psyr_3019 and Psyr_3020 are separated by 32 nucleotides Psyr_3020 and Psyr_3021 are separated by 84 nucleotides
Psyr_3017: Psyr_3017 - conserved hypothetical protein, at 3,614,086 to 3,614,268
_3017
Psyr_3018: Psyr_3018 - membrane protein, putative, at 3,614,279 to 3,615,001
_3018
Psyr_3019: Psyr_3019 - Cobalamin (vitamin B12) biosynthesis CbiG protein, at 3,614,979 to 3,615,428
_3019
Psyr_3020: Psyr_3020 - precorrin-4 C11-methyltransferase, at 3,615,461 to 3,616,210
_3020
Psyr_3021: Psyr_3021 - Pyruvate decarboxylase:Pyruvate ferredoxin/flavodoxin oxidoreductase, at 3,616,295 to 3,619,777
_3021
Position (kb)
3614
3615
3616 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3614.185 kb on + strand, within Psyr_3017 at 3614.185 kb on + strand, within Psyr_3017 at 3614.186 kb on - strand, within Psyr_3017 at 3614.297 kb on + strand at 3614.297 kb on + strand at 3614.298 kb on - strand at 3614.455 kb on + strand, within Psyr_3018 at 3614.456 kb on - strand, within Psyr_3018 at 3614.456 kb on - strand, within Psyr_3018 at 3614.567 kb on + strand at 3614.568 kb on - strand, within Psyr_3018 at 3614.568 kb on - strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.710 kb on - strand, within Psyr_3018 at 3615.470 kb on + strand at 3615.471 kb on - strand at 3615.471 kb on - strand at 3615.471 kb on - strand at 3615.550 kb on + strand, within Psyr_3020 at 3615.554 kb on + strand, within Psyr_3020 at 3615.869 kb on + strand, within Psyr_3020 at 3616.311 kb on + strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.312 kb on - strand at 3616.404 kb on + strand at 3616.404 kb on + strand at 3616.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 3,614,185 + Psyr_3017 0.54 -0.5 3,614,185 + Psyr_3017 0.54 +1.2 3,614,186 - Psyr_3017 0.55 -0.3 3,614,297 + -0.7 3,614,297 + -0.1 3,614,298 - +0.6 3,614,455 + Psyr_3018 0.24 -3.2 3,614,456 - Psyr_3018 0.24 -0.3 3,614,456 - Psyr_3018 0.24 +0.9 3,614,567 + +0.5 3,614,568 - Psyr_3018 0.40 +0.7 3,614,568 - Psyr_3018 0.40 +1.1 3,614,709 + Psyr_3018 0.59 +2.5 3,614,709 + Psyr_3018 0.59 +0.6 3,614,709 + Psyr_3018 0.59 +2.3 3,614,710 - Psyr_3018 0.60 +0.3 3,615,470 + -0.1 3,615,471 - +0.7 3,615,471 - -1.7 3,615,471 - -0.4 3,615,550 + Psyr_3020 0.12 +0.0 3,615,554 + Psyr_3020 0.12 -1.7 3,615,869 + Psyr_3020 0.54 -1.5 3,616,311 + -1.1 3,616,312 - +0.7 3,616,312 - -0.0 3,616,312 - +0.9 3,616,312 - -0.9 3,616,312 - +2.2 3,616,312 - +0.8 3,616,312 - -0.5 3,616,404 + -0.8 3,616,404 + -0.5 3,616,405 - -0.4
Or see this region's nucleotide sequence