Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3015 and Psyr_3016 are separated by 3 nucleotides Psyr_3016 and Psyr_3017 are separated by 715 nucleotides Psyr_3017 and Psyr_3018 are separated by 10 nucleotides
Psyr_3015: Psyr_3015 - cobaltochelatase CobN subunit, at 3,608,538 to 3,612,302
_3015
Psyr_3016: Psyr_3016 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 3,612,306 to 3,613,370
_3016
Psyr_3017: Psyr_3017 - conserved hypothetical protein, at 3,614,086 to 3,614,268
_3017
Psyr_3018: Psyr_3018 - membrane protein, putative, at 3,614,279 to 3,615,001
_3018
Position (kb)
3612
3613
3614 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3611.767 kb on + strand, within Psyr_3015 at 3611.767 kb on + strand, within Psyr_3015 at 3611.767 kb on + strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.845 kb on + strand, within Psyr_3015 at 3611.845 kb on + strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3612.091 kb on + strand at 3612.092 kb on - strand at 3612.092 kb on - strand at 3612.092 kb on - strand at 3612.152 kb on - strand at 3612.254 kb on + strand at 3612.254 kb on + strand at 3612.255 kb on - strand at 3612.294 kb on + strand at 3613.163 kb on - strand, within Psyr_3016 at 3613.163 kb on - strand, within Psyr_3016 at 3613.464 kb on + strand at 3613.550 kb on - strand at 3613.550 kb on - strand at 3613.661 kb on - strand at 3613.876 kb on + strand at 3613.876 kb on + strand at 3613.877 kb on - strand at 3613.877 kb on - strand at 3613.877 kb on - strand at 3613.974 kb on - strand at 3614.185 kb on + strand, within Psyr_3017 at 3614.185 kb on + strand, within Psyr_3017 at 3614.186 kb on - strand, within Psyr_3017 at 3614.297 kb on + strand at 3614.297 kb on + strand at 3614.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 3,611,767 + Psyr_3015 0.86 -2.1 3,611,767 + Psyr_3015 0.86 +0.6 3,611,767 + Psyr_3015 0.86 -0.7 3,611,768 - Psyr_3015 0.86 -0.6 3,611,768 - Psyr_3015 0.86 +2.5 3,611,768 - Psyr_3015 0.86 -0.2 3,611,845 + Psyr_3015 0.88 -0.5 3,611,845 + Psyr_3015 0.88 -1.2 3,611,846 - Psyr_3015 0.88 +0.4 3,611,846 - Psyr_3015 0.88 +0.7 3,611,846 - Psyr_3015 0.88 -1.0 3,611,846 - Psyr_3015 0.88 -0.4 3,612,091 + -0.1 3,612,092 - +0.9 3,612,092 - -1.6 3,612,092 - +2.9 3,612,152 - +0.4 3,612,254 + -1.7 3,612,254 + +0.2 3,612,255 - -0.6 3,612,294 + -0.6 3,613,163 - Psyr_3016 0.80 -0.3 3,613,163 - Psyr_3016 0.80 -2.9 3,613,464 + -1.3 3,613,550 - -0.9 3,613,550 - -0.1 3,613,661 - -0.4 3,613,876 + -1.1 3,613,876 + -0.6 3,613,877 - -1.3 3,613,877 - -0.2 3,613,877 - -0.5 3,613,974 - +0.7 3,614,185 + Psyr_3017 0.54 -0.5 3,614,185 + Psyr_3017 0.54 +1.2 3,614,186 - Psyr_3017 0.55 -0.3 3,614,297 + -0.7 3,614,297 + -0.1 3,614,298 - +0.6
Or see this region's nucleotide sequence