Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2299 and Psyr_2300 are separated by 53 nucleotides Psyr_2300 and Psyr_2301 overlap by 4 nucleotides Psyr_2301 and Psyr_2302 overlap by 4 nucleotides
Psyr_2299: Psyr_2299 - hypothetical protein, at 2,671,963 to 2,673,390
_2299
Psyr_2300: Psyr_2300 - Conserved hypothetical protein 374, at 2,673,444 to 2,674,412
_2300
Psyr_2301: Psyr_2301 - Short-chain dehydrogenase/reductase SDR, at 2,674,409 to 2,675,149
_2301
Psyr_2302: Psyr_2302 - FAD linked oxidase, N-terminal, at 2,675,146 to 2,676,462
_2302
Position (kb)
2673
2674
2675 Strain fitness (log2 ratio)
-1
0
1
2 at 2672.540 kb on - strand, within Psyr_2299 at 2672.711 kb on + strand, within Psyr_2299 at 2672.712 kb on - strand, within Psyr_2299 at 2672.712 kb on - strand, within Psyr_2299 at 2672.816 kb on - strand, within Psyr_2299 at 2672.816 kb on - strand, within Psyr_2299 at 2672.849 kb on + strand, within Psyr_2299 at 2672.849 kb on + strand, within Psyr_2299 at 2672.909 kb on - strand, within Psyr_2299 at 2672.963 kb on + strand, within Psyr_2299 at 2672.972 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.196 kb on - strand, within Psyr_2299 at 2673.361 kb on - strand at 2673.479 kb on + strand at 2673.537 kb on + strand at 2673.670 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.849 kb on - strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.337 kb on - strand at 2674.622 kb on + strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.623 kb on - strand, within Psyr_2301 at 2674.629 kb on - strand, within Psyr_2301 at 2674.704 kb on + strand, within Psyr_2301 at 2674.705 kb on - strand, within Psyr_2301 at 2674.983 kb on + strand, within Psyr_2301 at 2674.983 kb on + strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.984 kb on - strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.991 kb on + strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2674.992 kb on - strand, within Psyr_2301 at 2675.123 kb on - strand at 2675.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 2,672,540 - Psyr_2299 0.40 +1.3 2,672,711 + Psyr_2299 0.52 -1.5 2,672,712 - Psyr_2299 0.52 -0.3 2,672,712 - Psyr_2299 0.52 +0.4 2,672,816 - Psyr_2299 0.60 -1.3 2,672,816 - Psyr_2299 0.60 +1.1 2,672,849 + Psyr_2299 0.62 +1.0 2,672,849 + Psyr_2299 0.62 -0.1 2,672,909 - Psyr_2299 0.66 -0.8 2,672,963 + Psyr_2299 0.70 +0.8 2,672,972 + Psyr_2299 0.71 +0.7 2,673,005 + Psyr_2299 0.73 +1.3 2,673,005 + Psyr_2299 0.73 -0.8 2,673,005 + Psyr_2299 0.73 -0.2 2,673,196 - Psyr_2299 0.86 +0.1 2,673,361 - +0.4 2,673,479 + +1.8 2,673,537 + -0.8 2,673,670 + Psyr_2300 0.23 -0.5 2,673,674 + Psyr_2300 0.24 -1.4 2,673,674 + Psyr_2300 0.24 -0.5 2,673,678 + Psyr_2300 0.24 -0.2 2,673,678 + Psyr_2300 0.24 +0.0 2,673,849 - Psyr_2300 0.42 -1.6 2,674,150 + Psyr_2300 0.73 +2.0 2,674,150 + Psyr_2300 0.73 -0.8 2,674,201 + Psyr_2300 0.78 +0.2 2,674,201 + Psyr_2300 0.78 -0.4 2,674,337 - +1.3 2,674,622 + Psyr_2301 0.29 -0.2 2,674,623 - Psyr_2301 0.29 -0.2 2,674,623 - Psyr_2301 0.29 +1.9 2,674,623 - Psyr_2301 0.29 +0.4 2,674,623 - Psyr_2301 0.29 +0.0 2,674,629 - Psyr_2301 0.30 +0.3 2,674,704 + Psyr_2301 0.40 +1.5 2,674,705 - Psyr_2301 0.40 -0.9 2,674,983 + Psyr_2301 0.77 -0.5 2,674,983 + Psyr_2301 0.77 -1.2 2,674,984 - Psyr_2301 0.78 +0.0 2,674,984 - Psyr_2301 0.78 +0.0 2,674,984 - Psyr_2301 0.78 -0.4 2,674,991 + Psyr_2301 0.79 +1.4 2,674,991 + Psyr_2301 0.79 +1.0 2,674,991 + Psyr_2301 0.79 +2.0 2,674,992 - Psyr_2301 0.79 -0.6 2,674,992 - Psyr_2301 0.79 -0.4 2,674,992 - Psyr_2301 0.79 +1.8 2,674,992 - Psyr_2301 0.79 -0.6 2,675,123 - +1.2 2,675,123 - +0.9
Or see this region's nucleotide sequence