Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2226 and Psyr_2227 are separated by 297 nucleotides Psyr_2227 and Psyr_2228 are separated by 399 nucleotides
Psyr_2226: Psyr_2226 - formyltetrahydrofolate deformylase, at 2,587,752 to 2,588,618
_2226
Psyr_2227: Psyr_2227 - conserved hypothetical protein, at 2,588,916 to 2,589,371
_2227
Psyr_2228: Psyr_2228 - conserved hypothetical protein, at 2,589,771 to 2,590,499
_2228
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2587.942 kb on + strand at 2587.943 kb on - strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.189 kb on - strand, within Psyr_2226 at 2588.485 kb on + strand, within Psyr_2226 at 2588.486 kb on - strand at 2588.486 kb on - strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.967 kb on - strand, within Psyr_2227 at 2588.987 kb on + strand, within Psyr_2227 at 2589.282 kb on + strand, within Psyr_2227 at 2589.332 kb on + strand at 2589.332 kb on + strand at 2589.409 kb on + strand at 2589.410 kb on - strand at 2589.418 kb on + strand at 2589.443 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.454 kb on - strand at 2589.454 kb on - strand at 2589.457 kb on + strand at 2589.457 kb on + strand at 2589.474 kb on + strand at 2589.477 kb on - strand at 2589.521 kb on + strand at 2589.521 kb on + strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.728 kb on + strand at 2589.729 kb on - strand at 2589.729 kb on - strand at 2590.111 kb on + strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.127 kb on - strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.325 kb on - strand, within Psyr_2228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 2,587,942 + +0.0 2,587,943 - Psyr_2226 0.22 -0.4 2,588,188 + Psyr_2226 0.50 +0.1 2,588,188 + Psyr_2226 0.50 -0.0 2,588,189 - Psyr_2226 0.50 +0.0 2,588,485 + Psyr_2226 0.85 -0.8 2,588,486 - -1.6 2,588,486 - Psyr_2226 0.85 -1.1 2,588,518 + Psyr_2226 0.88 -0.3 2,588,519 - Psyr_2226 0.88 -0.7 2,588,519 - Psyr_2226 0.88 -0.2 2,588,736 + -0.2 2,588,736 + -1.4 2,588,736 + +1.5 2,588,736 + -0.2 2,588,737 - -0.3 2,588,737 - +0.1 2,588,737 - +0.7 2,588,889 - +0.6 2,588,889 - -1.0 2,588,918 - -0.5 2,588,918 - +1.6 2,588,918 - -0.4 2,588,967 - Psyr_2227 0.11 -0.4 2,588,987 + Psyr_2227 0.16 -1.6 2,589,282 + Psyr_2227 0.80 -0.7 2,589,332 + -0.8 2,589,332 + +0.2 2,589,409 + +0.3 2,589,410 - +0.8 2,589,418 + +0.3 2,589,443 + +0.0 2,589,453 + +0.2 2,589,453 + +0.8 2,589,453 + +1.0 2,589,453 + -1.6 2,589,454 - +1.8 2,589,454 - -0.5 2,589,457 + -1.0 2,589,457 + -0.6 2,589,474 + -1.1 2,589,477 - -0.3 2,589,521 + -0.6 2,589,521 + -0.9 2,589,522 - +0.4 2,589,522 - +0.0 2,589,522 - +0.6 2,589,728 + -1.0 2,589,729 - +0.7 2,589,729 - -0.5 2,590,111 + Psyr_2228 0.47 +1.9 2,590,112 - Psyr_2228 0.47 -0.4 2,590,112 - Psyr_2228 0.47 +1.6 2,590,112 - Psyr_2228 0.47 +0.1 2,590,112 - Psyr_2228 0.47 -1.2 2,590,126 + Psyr_2228 0.49 -0.4 2,590,126 + Psyr_2228 0.49 +1.6 2,590,127 - Psyr_2228 0.49 +0.9 2,590,136 + Psyr_2228 0.50 -0.4 2,590,136 + Psyr_2228 0.50 -0.7 2,590,137 - Psyr_2228 0.50 +3.5 2,590,137 - Psyr_2228 0.50 +1.0 2,590,325 - Psyr_2228 0.76 -0.2
Or see this region's nucleotide sequence