Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2171 and Psyr_2172 overlap by 4 nucleotides Psyr_2172 and Psyr_2173 are separated by 25 nucleotides Psyr_2173 and Psyr_2174 are separated by 0 nucleotides Psyr_2174 and Psyr_2175 are separated by 63 nucleotides
Psyr_2171: Psyr_2171 - HpcH/HpaI aldolase, at 2,524,631 to 2,525,446
_2171
Psyr_2172: Psyr_2172 - conserved hypothetical protein, at 2,525,443 to 2,526,288
_2172
Psyr_2173: Psyr_2173 - Electron transfer flavoprotein, alpha subunit, at 2,526,314 to 2,527,243
_2173
Psyr_2174: Psyr_2174 - Electron transfer flavoprotein beta-subunit, at 2,527,244 to 2,527,993
_2174
Psyr_2175: Psyr_2175 - L-carnitine dehydratase/bile acid-inducible protein F, at 2,528,057 to 2,529,280
_2175
Position (kb)
2526
2527
2528 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.708 kb on + strand, within Psyr_2172 at 2525.708 kb on + strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.720 kb on + strand, within Psyr_2172 at 2525.721 kb on - strand, within Psyr_2172 at 2525.727 kb on + strand, within Psyr_2172 at 2525.728 kb on - strand, within Psyr_2172 at 2525.728 kb on - strand, within Psyr_2172 at 2525.915 kb on - strand at 2525.929 kb on + strand, within Psyr_2172 at 2525.978 kb on - strand, within Psyr_2172 at 2526.004 kb on + strand, within Psyr_2172 at 2526.004 kb on + strand, within Psyr_2172 at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.470 kb on - strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.841 kb on + strand, within Psyr_2173 at 2526.841 kb on + strand, within Psyr_2173 at 2526.862 kb on - strand, within Psyr_2173 at 2526.862 kb on - strand, within Psyr_2173 at 2526.993 kb on + strand, within Psyr_2173 at 2527.014 kb on - strand, within Psyr_2173 at 2527.246 kb on - strand at 2527.251 kb on - strand at 2527.251 kb on - strand at 2527.441 kb on - strand, within Psyr_2174 at 2527.956 kb on + strand at 2527.956 kb on + strand at 2527.956 kb on + strand at 2527.956 kb on + strand at 2528.058 kb on + strand at 2528.098 kb on + strand at 2528.141 kb on + strand at 2528.161 kb on + strand at 2528.161 kb on + strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.162 kb on - strand at 2528.196 kb on - strand, within Psyr_2175 at 2528.196 kb on - strand, within Psyr_2175
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 2,525,671 + Psyr_2172 0.27 -0.0 2,525,671 + Psyr_2172 0.27 -1.3 2,525,671 + Psyr_2172 0.27 +0.1 2,525,671 + Psyr_2172 0.27 -0.3 2,525,672 - Psyr_2172 0.27 -0.5 2,525,672 - Psyr_2172 0.27 +1.0 2,525,672 - Psyr_2172 0.27 +0.3 2,525,672 - Psyr_2172 0.27 -0.8 2,525,708 + Psyr_2172 0.31 +0.1 2,525,708 + Psyr_2172 0.31 +2.0 2,525,709 - Psyr_2172 0.31 +1.0 2,525,709 - Psyr_2172 0.31 +0.9 2,525,709 - Psyr_2172 0.31 -0.3 2,525,720 + Psyr_2172 0.33 +0.5 2,525,721 - Psyr_2172 0.33 +0.3 2,525,727 + Psyr_2172 0.34 -0.2 2,525,728 - Psyr_2172 0.34 -0.2 2,525,728 - Psyr_2172 0.34 +0.4 2,525,915 - -0.6 2,525,929 + Psyr_2172 0.57 -0.8 2,525,978 - Psyr_2172 0.63 -1.9 2,526,004 + Psyr_2172 0.66 -0.4 2,526,004 + Psyr_2172 0.66 -0.7 2,526,288 + -1.0 2,526,288 + -0.6 2,526,288 + +1.0 2,526,288 + +1.9 2,526,470 - Psyr_2173 0.17 +1.3 2,526,483 + Psyr_2173 0.18 +0.1 2,526,483 + Psyr_2173 0.18 +0.2 2,526,483 + Psyr_2173 0.18 -0.6 2,526,483 + Psyr_2173 0.18 -0.2 2,526,483 + Psyr_2173 0.18 +2.6 2,526,483 + Psyr_2173 0.18 -0.5 2,526,484 - Psyr_2173 0.18 +0.2 2,526,484 - Psyr_2173 0.18 +0.2 2,526,484 - Psyr_2173 0.18 -1.7 2,526,484 - Psyr_2173 0.18 +0.7 2,526,484 - Psyr_2173 0.18 +0.1 2,526,484 - Psyr_2173 0.18 +1.0 2,526,484 - Psyr_2173 0.18 +0.8 2,526,841 + Psyr_2173 0.57 -1.4 2,526,841 + Psyr_2173 0.57 +0.2 2,526,862 - Psyr_2173 0.59 -1.9 2,526,862 - Psyr_2173 0.59 +0.2 2,526,993 + Psyr_2173 0.73 +0.5 2,527,014 - Psyr_2173 0.75 +2.1 2,527,246 - +0.4 2,527,251 - +1.0 2,527,251 - +0.4 2,527,441 - Psyr_2174 0.26 +1.4 2,527,956 + -0.0 2,527,956 + -0.1 2,527,956 + -1.8 2,527,956 + -1.7 2,528,058 + +1.9 2,528,098 + +0.1 2,528,141 + +0.3 2,528,161 + -0.5 2,528,161 + +1.0 2,528,162 - +0.1 2,528,162 - +0.0 2,528,162 - +2.0 2,528,162 - +1.2 2,528,162 - -0.9 2,528,162 - +0.0 2,528,162 - -0.4 2,528,162 - +0.1 2,528,196 - Psyr_2175 0.11 -0.1 2,528,196 - Psyr_2175 0.11 +0.4
Or see this region's nucleotide sequence