Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1838 and Psyr_1839 are separated by 28 nucleotides Psyr_1839 and Psyr_1840 are separated by 116 nucleotides Psyr_1840 and Psyr_1841 are separated by 82 nucleotides
Psyr_1838: Psyr_1838 - Peptidase U61, LD-carboxypeptidase A, at 2,118,451 to 2,119,386
_1838
Psyr_1839: Psyr_1839 - Benzoate membrane transport protein, at 2,119,415 to 2,120,608
_1839
Psyr_1840: Psyr_1840 - transcriptional regulator, MarR family, at 2,120,725 to 2,121,195
_1840
Psyr_1841: Psyr_1841 - Aminotransferase, class I and II, at 2,121,278 to 2,122,444
_1841
Position (kb)
2119
2120
2121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2118.416 kb on + strand at 2118.416 kb on + strand at 2118.722 kb on - strand, within Psyr_1838 at 2119.488 kb on - strand at 2119.717 kb on + strand, within Psyr_1839 at 2119.717 kb on + strand, within Psyr_1839 at 2119.717 kb on + strand, within Psyr_1839 at 2119.718 kb on - strand, within Psyr_1839 at 2119.719 kb on + strand, within Psyr_1839 at 2119.720 kb on - strand, within Psyr_1839 at 2119.855 kb on + strand, within Psyr_1839 at 2120.109 kb on + strand, within Psyr_1839 at 2120.109 kb on + strand, within Psyr_1839 at 2120.110 kb on - strand, within Psyr_1839 at 2120.110 kb on - strand, within Psyr_1839 at 2120.635 kb on + strand at 2120.655 kb on - strand at 2120.681 kb on + strand at 2120.682 kb on - strand at 2121.076 kb on + strand, within Psyr_1840 at 2121.076 kb on + strand, within Psyr_1840 at 2121.271 kb on - strand at 2121.271 kb on - strand at 2121.271 kb on - strand at 2121.578 kb on + strand, within Psyr_1841 at 2121.578 kb on + strand, within Psyr_1841 at 2121.578 kb on + strand, within Psyr_1841 at 2121.578 kb on + strand, within Psyr_1841 at 2121.579 kb on - strand, within Psyr_1841
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 2,118,416 + +0.5 2,118,416 + +1.6 2,118,722 - Psyr_1838 0.29 -3.5 2,119,488 - -0.1 2,119,717 + Psyr_1839 0.25 +0.3 2,119,717 + Psyr_1839 0.25 +0.2 2,119,717 + Psyr_1839 0.25 -1.3 2,119,718 - Psyr_1839 0.25 +1.5 2,119,719 + Psyr_1839 0.25 -0.3 2,119,720 - Psyr_1839 0.26 -0.6 2,119,855 + Psyr_1839 0.37 +1.3 2,120,109 + Psyr_1839 0.58 +2.5 2,120,109 + Psyr_1839 0.58 +0.6 2,120,110 - Psyr_1839 0.58 -0.6 2,120,110 - Psyr_1839 0.58 -0.5 2,120,635 + +0.5 2,120,655 - +0.9 2,120,681 + -0.8 2,120,682 - +0.1 2,121,076 + Psyr_1840 0.75 -0.2 2,121,076 + Psyr_1840 0.75 +0.2 2,121,271 - +1.6 2,121,271 - +0.9 2,121,271 - +0.9 2,121,578 + Psyr_1841 0.26 +0.6 2,121,578 + Psyr_1841 0.26 +0.3 2,121,578 + Psyr_1841 0.26 -0.1 2,121,578 + Psyr_1841 0.26 -1.4 2,121,579 - Psyr_1841 0.26 -1.7
Or see this region's nucleotide sequence