Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1686 and Psyr_1687 are separated by 2 nucleotides Psyr_1687 and Psyr_1688 are separated by 28 nucleotides Psyr_1688 and Psyr_1689 are separated by 29 nucleotides Psyr_1689 and Psyr_1690 are separated by 12 nucleotides
Psyr_1686: Psyr_1686 - Metallophosphoesterase, at 1,894,769 to 1,895,734
_1686
Psyr_1687: Psyr_1687 - Rhomboid-like protein, at 1,895,737 to 1,896,609
_1687
Psyr_1688: Psyr_1688 - Protein of unknown function DUF1315, at 1,896,638 to 1,896,898
_1688
Psyr_1689: Psyr_1689 - conserved hypothetical protein, at 1,896,928 to 1,897,728
_1689
Psyr_1690: Psyr_1690 - Peptidase M1, membrane alanine aminopeptidase, at 1,897,741 to 1,900,407
_1690
Position (kb)
1896
1897 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1895.747 kb on + strand at 1895.748 kb on - strand at 1895.748 kb on - strand at 1895.748 kb on - strand at 1895.748 kb on - strand at 1895.902 kb on + strand, within Psyr_1687 at 1895.965 kb on + strand, within Psyr_1687 at 1895.965 kb on + strand, within Psyr_1687 at 1895.965 kb on + strand, within Psyr_1687 at 1895.965 kb on + strand, within Psyr_1687 at 1895.965 kb on + strand, within Psyr_1687 at 1895.966 kb on - strand, within Psyr_1687 at 1895.993 kb on + strand, within Psyr_1687 at 1896.332 kb on - strand, within Psyr_1687 at 1896.379 kb on + strand, within Psyr_1687 at 1896.379 kb on + strand, within Psyr_1687 at 1896.379 kb on + strand, within Psyr_1687 at 1896.607 kb on + strand at 1896.614 kb on - strand at 1896.650 kb on - strand at 1896.650 kb on - strand at 1896.650 kb on - strand at 1896.652 kb on - strand at 1896.684 kb on - strand, within Psyr_1688 at 1896.684 kb on - strand, within Psyr_1688 at 1896.684 kb on - strand, within Psyr_1688 at 1896.684 kb on - strand, within Psyr_1688 at 1896.964 kb on + strand at 1897.084 kb on + strand, within Psyr_1689 at 1897.096 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.231 kb on + strand, within Psyr_1689 at 1897.535 kb on + strand, within Psyr_1689 at 1897.535 kb on + strand, within Psyr_1689 at 1897.536 kb on - strand, within Psyr_1689 at 1897.536 kb on - strand, within Psyr_1689 at 1897.658 kb on - strand at 1897.658 kb on - strand at 1897.696 kb on + strand at 1897.697 kb on - strand at 1897.697 kb on - strand at 1897.706 kb on - strand at 1897.706 kb on - strand at 1897.706 kb on - strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.726 kb on + strand at 1897.727 kb on - strand at 1897.727 kb on - strand at 1897.768 kb on + strand at 1897.780 kb on + strand at 1897.781 kb on - strand at 1897.796 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 1,895,747 + +0.2 1,895,748 - -0.9 1,895,748 - +0.7 1,895,748 - +0.7 1,895,748 - -0.3 1,895,902 + Psyr_1687 0.19 -0.6 1,895,965 + Psyr_1687 0.26 +1.3 1,895,965 + Psyr_1687 0.26 +1.3 1,895,965 + Psyr_1687 0.26 +0.3 1,895,965 + Psyr_1687 0.26 +3.3 1,895,965 + Psyr_1687 0.26 -1.9 1,895,966 - Psyr_1687 0.26 -0.7 1,895,993 + Psyr_1687 0.29 +0.9 1,896,332 - Psyr_1687 0.68 +1.3 1,896,379 + Psyr_1687 0.74 -1.0 1,896,379 + Psyr_1687 0.74 +0.3 1,896,379 + Psyr_1687 0.74 -1.2 1,896,607 + -0.2 1,896,614 - -0.3 1,896,650 - -0.5 1,896,650 - -0.5 1,896,650 - -1.7 1,896,652 - +1.2 1,896,684 - Psyr_1688 0.18 -1.1 1,896,684 - Psyr_1688 0.18 -0.1 1,896,684 - Psyr_1688 0.18 -0.5 1,896,684 - Psyr_1688 0.18 -2.7 1,896,964 + +0.5 1,897,084 + Psyr_1689 0.19 -0.3 1,897,096 + Psyr_1689 0.21 +1.0 1,897,231 + Psyr_1689 0.38 +1.7 1,897,231 + Psyr_1689 0.38 -1.0 1,897,231 + Psyr_1689 0.38 +0.4 1,897,535 + Psyr_1689 0.76 +0.6 1,897,535 + Psyr_1689 0.76 +3.1 1,897,536 - Psyr_1689 0.76 -0.1 1,897,536 - Psyr_1689 0.76 +0.3 1,897,658 - +1.3 1,897,658 - -0.8 1,897,696 + +0.9 1,897,697 - +0.1 1,897,697 - -1.3 1,897,706 - +1.1 1,897,706 - +0.2 1,897,706 - +0.4 1,897,726 + +0.3 1,897,726 + +0.7 1,897,726 + -0.9 1,897,726 + +0.9 1,897,726 + -1.9 1,897,727 - +0.3 1,897,727 - +0.1 1,897,768 + -1.2 1,897,780 + +0.8 1,897,781 - -0.1 1,897,796 - -0.9
Or see this region's nucleotide sequence