Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1482 and Psyr_1483 are separated by 509 nucleotides Psyr_1483 and Psyr_1484 are separated by 42 nucleotides Psyr_1484 and Psyr_1485 are separated by 80 nucleotides Psyr_1485 and Psyr_1486 overlap by 4 nucleotides
Psyr_1482: Psyr_1482 - enolase, at 1,670,787 to 1,672,070
_1482
Psyr_1483: Psyr_1483 - hypothetical protein, at 1,672,580 to 1,672,846
_1483
Psyr_1484: Psyr_1484 - conserved hypothetical protein, at 1,672,889 to 1,673,212
_1484
Psyr_1485: Psyr_1485 - hypothetical protein, at 1,673,293 to 1,673,658
_1485
Psyr_1486: Psyr_1486 - Cl- channel, voltage gated, at 1,673,655 to 1,675,001
_1486
Position (kb) 1672
1673 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1671.624 kb on - strand, within Psyr_1482 at 1671.671 kb on - strand, within Psyr_1482 at 1671.671 kb on - strand, within Psyr_1482 at 1671.782 kb on + strand, within Psyr_1482 at 1671.782 kb on + strand, within Psyr_1482 at 1671.783 kb on - strand, within Psyr_1482 at 1671.843 kb on + strand, within Psyr_1482 at 1671.843 kb on + strand, within Psyr_1482 at 1671.843 kb on + strand, within Psyr_1482 at 1671.843 kb on + strand, within Psyr_1482 at 1671.844 kb on - strand, within Psyr_1482 at 1671.844 kb on - strand, within Psyr_1482 at 1671.874 kb on - strand, within Psyr_1482 at 1671.902 kb on + strand, within Psyr_1482 at 1671.903 kb on - strand, within Psyr_1482 at 1671.903 kb on - strand, within Psyr_1482 at 1671.941 kb on + strand, within Psyr_1482 at 1672.010 kb on + strand at 1672.133 kb on + strand at 1672.133 kb on + strand at 1672.133 kb on + strand at 1672.133 kb on + strand at 1672.133 kb on + strand at 1672.133 kb on + strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.134 kb on - strand at 1672.160 kb on - strand at 1672.215 kb on + strand at 1672.215 kb on + strand at 1672.219 kb on + strand at 1672.219 kb on + strand at 1672.219 kb on + strand at 1672.220 kb on - strand at 1672.220 kb on - strand at 1672.220 kb on - strand at 1672.307 kb on - strand at 1672.336 kb on + strand at 1672.336 kb on + strand at 1672.337 kb on - strand at 1672.357 kb on + strand at 1672.358 kb on - strand at 1672.358 kb on - strand at 1672.393 kb on + strand at 1672.418 kb on + strand at 1672.419 kb on - strand at 1672.419 kb on - strand at 1672.419 kb on - strand at 1672.419 kb on - strand at 1672.459 kb on + strand at 1672.503 kb on + strand at 1672.628 kb on + strand, within Psyr_1483 at 1672.629 kb on - strand, within Psyr_1483 at 1672.660 kb on + strand, within Psyr_1483 at 1672.661 kb on - strand, within Psyr_1483 at 1672.661 kb on - strand, within Psyr_1483 at 1672.674 kb on - strand, within Psyr_1483 at 1672.701 kb on + strand, within Psyr_1483 at 1672.702 kb on - strand, within Psyr_1483 at 1672.702 kb on - strand, within Psyr_1483 at 1672.773 kb on - strand, within Psyr_1483 at 1672.826 kb on + strand at 1672.826 kb on + strand at 1672.826 kb on + strand at 1672.826 kb on + strand at 1672.826 kb on + strand at 1672.826 kb on + strand at 1672.827 kb on - strand at 1672.827 kb on - strand at 1672.827 kb on - strand at 1672.827 kb on - strand at 1672.827 kb on - strand at 1672.832 kb on - strand at 1672.833 kb on + strand at 1672.834 kb on - strand at 1672.842 kb on + strand at 1672.843 kb on - strand at 1672.918 kb on - strand at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.938 kb on + strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1672.939 kb on - strand, within Psyr_1484 at 1673.007 kb on - strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.008 kb on + strand, within Psyr_1484 at 1673.009 kb on - strand, within Psyr_1484 at 1673.009 kb on - strand, within Psyr_1484 at 1673.009 kb on - strand, within Psyr_1484 at 1673.009 kb on - strand, within Psyr_1484 at 1673.075 kb on + strand, within Psyr_1484 at 1673.076 kb on - strand, within Psyr_1484 at 1673.174 kb on - strand, within Psyr_1484 at 1673.174 kb on - strand, within Psyr_1484 at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.212 kb on + strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.213 kb on - strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.216 kb on + strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.217 kb on - strand at 1673.225 kb on + strand at 1673.226 kb on - strand at 1673.259 kb on + strand at 1673.259 kb on + strand at 1673.260 kb on - strand at 1673.294 kb on + strand at 1673.294 kb on + strand at 1673.295 kb on - strand at 1673.295 kb on - strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.323 kb on + strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.324 kb on - strand at 1673.375 kb on + strand, within Psyr_1485 at 1673.375 kb on + strand, within Psyr_1485 at 1673.375 kb on + strand, within Psyr_1485 at 1673.397 kb on + strand, within Psyr_1485 at 1673.397 kb on + strand, within Psyr_1485 at 1673.398 kb on - strand, within Psyr_1485 at 1673.398 kb on - strand, within Psyr_1485 at 1673.398 kb on - strand, within Psyr_1485 at 1673.398 kb on - strand, within Psyr_1485 at 1673.398 kb on - strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.401 kb on + strand, within Psyr_1485 at 1673.402 kb on - strand, within Psyr_1485 at 1673.402 kb on - strand, within Psyr_1485 at 1673.402 kb on - strand, within Psyr_1485 at 1673.402 kb on - strand, within Psyr_1485 at 1673.402 kb on - strand, within Psyr_1485 at 1673.477 kb on + strand, within Psyr_1485 at 1673.477 kb on + strand, within Psyr_1485 at 1673.535 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.566 kb on + strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.567 kb on - strand, within Psyr_1485 at 1673.615 kb on + strand, within Psyr_1485 at 1673.616 kb on - strand, within Psyr_1485 at 1673.726 kb on + strand at 1673.726 kb on + strand at 1673.794 kb on + strand, within Psyr_1486 at 1673.794 kb on + strand, within Psyr_1486 at 1673.795 kb on - strand, within Psyr_1486 at 1673.795 kb on - strand, within Psyr_1486 at 1673.795 kb on - strand, within Psyr_1486 at 1673.795 kb on - strand, within Psyr_1486 at 1673.821 kb on + strand, within Psyr_1486
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 1,671,624 - Psyr_1482 0.65 +2.0 1,671,671 - Psyr_1482 0.69 +0.2 1,671,671 - Psyr_1482 0.69 -0.0 1,671,782 + Psyr_1482 0.77 -1.4 1,671,782 + Psyr_1482 0.77 +0.0 1,671,783 - Psyr_1482 0.78 -1.0 1,671,843 + Psyr_1482 0.82 -0.7 1,671,843 + Psyr_1482 0.82 -0.8 1,671,843 + Psyr_1482 0.82 +0.4 1,671,843 + Psyr_1482 0.82 +1.8 1,671,844 - Psyr_1482 0.82 +0.1 1,671,844 - Psyr_1482 0.82 -0.3 1,671,874 - Psyr_1482 0.85 +1.6 1,671,902 + Psyr_1482 0.87 -1.2 1,671,903 - Psyr_1482 0.87 -0.9 1,671,903 - Psyr_1482 0.87 -0.9 1,671,941 + Psyr_1482 0.90 -0.6 1,672,010 + +0.4 1,672,133 + -0.4 1,672,133 + +0.3 1,672,133 + +1.1 1,672,133 + +0.4 1,672,133 + -1.2 1,672,133 + -0.6 1,672,134 - -0.4 1,672,134 - -0.9 1,672,134 - +0.3 1,672,134 - -0.3 1,672,134 - -1.2 1,672,134 - -3.1 1,672,134 - +1.6 1,672,134 - +2.0 1,672,134 - -1.1 1,672,134 - +0.4 1,672,160 - +2.6 1,672,215 + +0.5 1,672,215 + -0.5 1,672,219 + +1.0 1,672,219 + -0.9 1,672,219 + +2.3 1,672,220 - +0.1 1,672,220 - +0.9 1,672,220 - +1.8 1,672,307 - +0.0 1,672,336 + +0.8 1,672,336 + -0.1 1,672,337 - +0.0 1,672,357 + +0.3 1,672,358 - +0.2 1,672,358 - +0.3 1,672,393 + +0.1 1,672,418 + -0.6 1,672,419 - -0.4 1,672,419 - +0.7 1,672,419 - -1.2 1,672,419 - -0.8 1,672,459 + -0.3 1,672,503 + -0.6 1,672,628 + Psyr_1483 0.18 -0.6 1,672,629 - Psyr_1483 0.18 -1.0 1,672,660 + Psyr_1483 0.30 -0.2 1,672,661 - Psyr_1483 0.30 -0.6 1,672,661 - Psyr_1483 0.30 -0.7 1,672,674 - Psyr_1483 0.35 -1.1 1,672,701 + Psyr_1483 0.45 -1.2 1,672,702 - Psyr_1483 0.46 +0.1 1,672,702 - Psyr_1483 0.46 -0.9 1,672,773 - Psyr_1483 0.72 -1.2 1,672,826 + +2.6 1,672,826 + -0.6 1,672,826 + -0.1 1,672,826 + -1.2 1,672,826 + -0.5 1,672,826 + -0.3 1,672,827 - -1.1 1,672,827 - +0.2 1,672,827 - +0.4 1,672,827 - +1.3 1,672,827 - -0.4 1,672,832 - -1.1 1,672,833 + -0.5 1,672,834 - -1.3 1,672,842 + +0.9 1,672,843 - -0.8 1,672,918 - -0.1 1,672,938 + Psyr_1484 0.15 -0.0 1,672,938 + Psyr_1484 0.15 -0.6 1,672,938 + Psyr_1484 0.15 -0.4 1,672,938 + Psyr_1484 0.15 -1.1 1,672,938 + Psyr_1484 0.15 -0.1 1,672,938 + Psyr_1484 0.15 +0.3 1,672,938 + Psyr_1484 0.15 +0.1 1,672,938 + Psyr_1484 0.15 +1.5 1,672,938 + Psyr_1484 0.15 -0.1 1,672,938 + Psyr_1484 0.15 -1.2 1,672,939 - Psyr_1484 0.15 -1.2 1,672,939 - Psyr_1484 0.15 -0.8 1,672,939 - Psyr_1484 0.15 -0.4 1,672,939 - Psyr_1484 0.15 -1.1 1,672,939 - Psyr_1484 0.15 +0.2 1,672,939 - Psyr_1484 0.15 +1.0 1,672,939 - Psyr_1484 0.15 -0.6 1,672,939 - Psyr_1484 0.15 +0.8 1,672,939 - Psyr_1484 0.15 -0.7 1,672,939 - Psyr_1484 0.15 +1.6 1,673,007 - Psyr_1484 0.36 -0.3 1,673,008 + Psyr_1484 0.37 -1.4 1,673,008 + Psyr_1484 0.37 -1.2 1,673,008 + Psyr_1484 0.37 -0.1 1,673,008 + Psyr_1484 0.37 +1.0 1,673,008 + Psyr_1484 0.37 -0.6 1,673,008 + Psyr_1484 0.37 -0.4 1,673,008 + Psyr_1484 0.37 -0.4 1,673,009 - Psyr_1484 0.37 -1.2 1,673,009 - Psyr_1484 0.37 -0.3 1,673,009 - Psyr_1484 0.37 -1.6 1,673,009 - Psyr_1484 0.37 -0.6 1,673,075 + Psyr_1484 0.57 -0.9 1,673,076 - Psyr_1484 0.58 -1.0 1,673,174 - Psyr_1484 0.88 -1.5 1,673,174 - Psyr_1484 0.88 +0.4 1,673,212 + +0.4 1,673,212 + +0.0 1,673,212 + -1.3 1,673,212 + -0.7 1,673,212 + +1.5 1,673,212 + -0.1 1,673,212 + +1.4 1,673,213 - -3.7 1,673,213 - -0.7 1,673,213 - -1.3 1,673,213 - -0.8 1,673,213 - -0.6 1,673,213 - -0.4 1,673,213 - -0.6 1,673,216 + +0.5 1,673,216 + +0.5 1,673,216 + +0.2 1,673,216 + -0.7 1,673,216 + +0.2 1,673,216 + +0.0 1,673,216 + -1.2 1,673,217 - -2.0 1,673,217 - +2.0 1,673,217 - -0.5 1,673,217 - -0.4 1,673,217 - -1.3 1,673,217 - -1.5 1,673,217 - -0.3 1,673,217 - -0.7 1,673,217 - -0.8 1,673,217 - -0.1 1,673,217 - -2.4 1,673,217 - +0.0 1,673,217 - +0.5 1,673,217 - -0.5 1,673,217 - -0.3 1,673,217 - -1.1 1,673,217 - -1.9 1,673,217 - -0.1 1,673,217 - -0.7 1,673,217 - -0.2 1,673,225 + +0.4 1,673,226 - -0.8 1,673,259 + -1.6 1,673,259 + +2.0 1,673,260 - +0.0 1,673,294 + -3.1 1,673,294 + -2.0 1,673,295 - +0.5 1,673,295 - -1.5 1,673,323 + +1.0 1,673,323 + -1.1 1,673,323 + +1.0 1,673,323 + -1.0 1,673,323 + +0.8 1,673,323 + +0.0 1,673,323 + +0.8 1,673,323 + -0.6 1,673,324 - -0.0 1,673,324 - +0.0 1,673,324 - +0.1 1,673,324 - -0.9 1,673,324 - +2.5 1,673,324 - -1.3 1,673,324 - -2.4 1,673,375 + Psyr_1485 0.22 +0.6 1,673,375 + Psyr_1485 0.22 -0.9 1,673,375 + Psyr_1485 0.22 -0.4 1,673,397 + Psyr_1485 0.28 +1.4 1,673,397 + Psyr_1485 0.28 -0.8 1,673,398 - Psyr_1485 0.29 -1.3 1,673,398 - Psyr_1485 0.29 -1.0 1,673,398 - Psyr_1485 0.29 +0.6 1,673,398 - Psyr_1485 0.29 -1.1 1,673,398 - Psyr_1485 0.29 -0.9 1,673,401 + Psyr_1485 0.30 -0.8 1,673,401 + Psyr_1485 0.30 -1.3 1,673,401 + Psyr_1485 0.30 +1.9 1,673,401 + Psyr_1485 0.30 +0.9 1,673,401 + Psyr_1485 0.30 +0.4 1,673,401 + Psyr_1485 0.30 +1.2 1,673,402 - Psyr_1485 0.30 -1.7 1,673,402 - Psyr_1485 0.30 -2.0 1,673,402 - Psyr_1485 0.30 -0.7 1,673,402 - Psyr_1485 0.30 -3.4 1,673,402 - Psyr_1485 0.30 -0.8 1,673,477 + Psyr_1485 0.50 -0.3 1,673,477 + Psyr_1485 0.50 +1.0 1,673,535 + Psyr_1485 0.66 -0.3 1,673,566 + Psyr_1485 0.75 -2.1 1,673,566 + Psyr_1485 0.75 +0.0 1,673,566 + Psyr_1485 0.75 -0.2 1,673,566 + Psyr_1485 0.75 +2.1 1,673,566 + Psyr_1485 0.75 -1.6 1,673,566 + Psyr_1485 0.75 +0.7 1,673,567 - Psyr_1485 0.75 +0.8 1,673,567 - Psyr_1485 0.75 +1.0 1,673,567 - Psyr_1485 0.75 +0.0 1,673,567 - Psyr_1485 0.75 +3.2 1,673,567 - Psyr_1485 0.75 +0.0 1,673,567 - Psyr_1485 0.75 -0.0 1,673,615 + Psyr_1485 0.88 +0.2 1,673,616 - Psyr_1485 0.88 -0.6 1,673,726 + -0.6 1,673,726 + +0.4 1,673,794 + Psyr_1486 0.10 +1.6 1,673,794 + Psyr_1486 0.10 -0.3 1,673,795 - Psyr_1486 0.10 -1.7 1,673,795 - Psyr_1486 0.10 -0.1 1,673,795 - Psyr_1486 0.10 -1.6 1,673,795 - Psyr_1486 0.10 -0.2 1,673,821 + Psyr_1486 0.12 +0.2
Or see this region's nucleotide sequence