Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1370

Experiment: KB with Avocatin B 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1368 and Psyr_1369 are separated by 53 nucleotidesPsyr_1369 and Psyr_1370 overlap by 4 nucleotidesPsyr_1370 and Psyr_1371 overlap by 13 nucleotidesPsyr_1371 and Psyr_1372 overlap by 1 nucleotides Psyr_1368: Psyr_1368 - Carboxylesterase, at 1,551,681 to 1,552,526 _1368 Psyr_1369: Psyr_1369 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,552,580 to 1,553,053 _1369 Psyr_1370: Psyr_1370 - tRNA pseudouridine synthase D, TruD, at 1,553,050 to 1,554,108 _1370 Psyr_1371: Psyr_1371 - 5'-nucleotidase / exopolyphosphatase / 3'-nucleotidase, at 1,554,096 to 1,554,845 _1371 Psyr_1372: Psyr_1372 - Protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 1,554,845 to 1,555,522 _1372 Position (kb) 1553 1554 1555Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1552.053 kb on + strand, within Psyr_1368at 1552.053 kb on + strand, within Psyr_1368at 1552.053 kb on + strand, within Psyr_1368at 1552.053 kb on + strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.054 kb on - strand, within Psyr_1368at 1552.461 kb on + strandat 1552.461 kb on + strandat 1552.461 kb on + strandat 1552.461 kb on + strandat 1552.462 kb on - strandat 1552.462 kb on - strandat 1552.462 kb on - strandat 1552.462 kb on - strandat 1552.524 kb on + strandat 1552.543 kb on + strandat 1552.543 kb on + strandat 1553.083 kb on + strandat 1553.531 kb on - strand, within Psyr_1370at 1553.531 kb on - strand, within Psyr_1370at 1553.657 kb on - strand, within Psyr_1370at 1553.877 kb on + strand, within Psyr_1370at 1553.877 kb on + strand, within Psyr_1370at 1553.877 kb on + strand, within Psyr_1370at 1553.877 kb on + strand, within Psyr_1370at 1553.878 kb on - strand, within Psyr_1370at 1554.101 kb on + strandat 1554.102 kb on - strandat 1554.328 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.394 kb on + strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.395 kb on - strand, within Psyr_1371at 1554.400 kb on - strand, within Psyr_1371at 1554.486 kb on - strand, within Psyr_1371at 1554.508 kb on - strand, within Psyr_1371at 1554.575 kb on + strand, within Psyr_1371at 1554.575 kb on + strand, within Psyr_1371at 1554.752 kb on - strand, within Psyr_1371at 1554.752 kb on - strand, within Psyr_1371at 1554.759 kb on + strand, within Psyr_1371at 1554.759 kb on + strand, within Psyr_1371at 1554.759 kb on + strand, within Psyr_1371at 1554.760 kb on - strand, within Psyr_1371at 1554.760 kb on - strand, within Psyr_1371at 1554.763 kb on + strand, within Psyr_1371at 1554.867 kb on + strandat 1554.868 kb on - strandat 1555.073 kb on + strand, within Psyr_1372at 1555.073 kb on + strand, within Psyr_1372at 1555.074 kb on - strand, within Psyr_1372

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM
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1,552,053 + Psyr_1368 0.44 -1.0
1,552,053 + Psyr_1368 0.44 -1.0
1,552,053 + Psyr_1368 0.44 -1.3
1,552,053 + Psyr_1368 0.44 -0.3
1,552,054 - Psyr_1368 0.44 +0.0
1,552,054 - Psyr_1368 0.44 +1.0
1,552,054 - Psyr_1368 0.44 +0.3
1,552,054 - Psyr_1368 0.44 -0.0
1,552,054 - Psyr_1368 0.44 +0.7
1,552,054 - Psyr_1368 0.44 -1.6
1,552,461 + +0.3
1,552,461 + +0.0
1,552,461 + +2.0
1,552,461 + +0.0
1,552,462 - -1.8
1,552,462 - -0.0
1,552,462 - +1.2
1,552,462 - -1.0
1,552,524 + +0.4
1,552,543 + +1.2
1,552,543 + +0.8
1,553,083 + +1.6
1,553,531 - Psyr_1370 0.45 +0.1
1,553,531 - Psyr_1370 0.45 +2.4
1,553,657 - Psyr_1370 0.57 -0.1
1,553,877 + Psyr_1370 0.78 +0.5
1,553,877 + Psyr_1370 0.78 -0.4
1,553,877 + Psyr_1370 0.78 -0.9
1,553,877 + Psyr_1370 0.78 -0.2
1,553,878 - Psyr_1370 0.78 +0.0
1,554,101 + -0.2
1,554,102 - -3.6
1,554,328 + Psyr_1371 0.31 +0.5
1,554,394 + Psyr_1371 0.40 -0.8
1,554,394 + Psyr_1371 0.40 -0.8
1,554,394 + Psyr_1371 0.40 -3.2
1,554,394 + Psyr_1371 0.40 +0.0
1,554,394 + Psyr_1371 0.40 -0.2
1,554,394 + Psyr_1371 0.40 +0.7
1,554,394 + Psyr_1371 0.40 -0.6
1,554,394 + Psyr_1371 0.40 -1.4
1,554,394 + Psyr_1371 0.40 -1.3
1,554,394 + Psyr_1371 0.40 -1.6
1,554,394 + Psyr_1371 0.40 -1.5
1,554,394 + Psyr_1371 0.40 +0.2
1,554,394 + Psyr_1371 0.40 -2.6
1,554,395 - Psyr_1371 0.40 -2.0
1,554,395 - Psyr_1371 0.40 +0.0
1,554,395 - Psyr_1371 0.40 -1.0
1,554,395 - Psyr_1371 0.40 -3.2
1,554,395 - Psyr_1371 0.40 -2.2
1,554,395 - Psyr_1371 0.40 -0.8
1,554,395 - Psyr_1371 0.40 -2.6
1,554,395 - Psyr_1371 0.40 -0.3
1,554,395 - Psyr_1371 0.40 -0.7
1,554,395 - Psyr_1371 0.40 -1.6
1,554,400 - Psyr_1371 0.41 -1.4
1,554,486 - Psyr_1371 0.52 -2.2
1,554,508 - Psyr_1371 0.55 -1.2
1,554,575 + Psyr_1371 0.64 -0.7
1,554,575 + Psyr_1371 0.64 -1.2
1,554,752 - Psyr_1371 0.87 -2.9
1,554,752 - Psyr_1371 0.87 -1.3
1,554,759 + Psyr_1371 0.88 -0.9
1,554,759 + Psyr_1371 0.88 -1.6
1,554,759 + Psyr_1371 0.88 -0.9
1,554,760 - Psyr_1371 0.89 -3.1
1,554,760 - Psyr_1371 0.89 -1.9
1,554,763 + Psyr_1371 0.89 -0.8
1,554,867 + +1.4
1,554,868 - +0.2
1,555,073 + Psyr_1372 0.34 -0.5
1,555,073 + Psyr_1372 0.34 -0.6
1,555,074 - Psyr_1372 0.34 -0.8

Or see this region's nucleotide sequence