Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1016 and Psyr_1017 are separated by 113 nucleotides Psyr_1017 and Psyr_1018 overlap by 8 nucleotides Psyr_1018 and Psyr_1019 are separated by 43 nucleotides Psyr_1019 and Psyr_1020 overlap by 1 nucleotides
Psyr_1016: Psyr_1016 - Thioredoxin reductase, at 1,149,858 to 1,150,820
_1016
Psyr_1017: Psyr_1017 - type III effector HopJ1, at 1,150,934 to 1,151,275
_1017
Psyr_1018: Psyr_1018 - Protein of unknown function DUF1244, at 1,151,268 to 1,151,567
_1018
Psyr_1019: Psyr_1019 - Dihydroneopterin aldolase, at 1,151,611 to 1,151,982
_1019
Psyr_1020: Psyr_1020 - GTP cyclohydrolase I, at 1,151,982 to 1,152,545
_1020
Position (kb) 1150
1151
1152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1150.810 kb on + strand at 1150.889 kb on + strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1151.190 kb on + strand, within Psyr_1017 at 1151.190 kb on + strand, within Psyr_1017 at 1151.353 kb on + strand, within Psyr_1018 at 1151.366 kb on - strand, within Psyr_1018 at 1151.366 kb on - strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.603 kb on + strand at 1151.604 kb on - strand at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.805 kb on - strand, within Psyr_1019 at 1151.805 kb on - strand, within Psyr_1019 at 1151.856 kb on - strand, within Psyr_1019 at 1151.856 kb on - strand, within Psyr_1019 at 1151.983 kb on + strand at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.139 kb on - strand, within Psyr_1020 at 1152.139 kb on - strand, within Psyr_1020
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 1,150,810 + +2.6 1,150,889 + +0.6 1,150,890 - +1.2 1,150,890 - +0.4 1,150,890 - +0.1 1,150,890 - +0.5 1,150,891 + +0.8 1,150,891 + -0.6 1,150,891 + -0.6 1,150,891 + -0.5 1,150,891 + +1.0 1,150,891 + -0.1 1,150,891 + -0.1 1,150,891 + -3.1 1,150,891 + -1.1 1,150,892 - -1.6 1,150,892 - +0.6 1,150,892 - +2.6 1,150,892 - -0.0 1,150,892 - -1.2 1,150,892 - +1.6 1,151,190 + Psyr_1017 0.75 -0.4 1,151,190 + Psyr_1017 0.75 -1.6 1,151,353 + Psyr_1018 0.28 +0.3 1,151,366 - Psyr_1018 0.33 +0.7 1,151,366 - Psyr_1018 0.33 +0.0 1,151,428 + Psyr_1018 0.53 -0.3 1,151,428 + Psyr_1018 0.53 -0.4 1,151,428 + Psyr_1018 0.53 -0.3 1,151,429 - Psyr_1018 0.54 +0.6 1,151,429 - Psyr_1018 0.54 -1.1 1,151,429 - Psyr_1018 0.54 -0.2 1,151,603 + -0.3 1,151,604 - +1.0 1,151,690 + Psyr_1019 0.21 +0.1 1,151,690 + Psyr_1019 0.21 +0.3 1,151,690 + Psyr_1019 0.21 +0.2 1,151,690 + Psyr_1019 0.21 -0.2 1,151,690 + Psyr_1019 0.21 +0.2 1,151,690 + Psyr_1019 0.21 -0.9 1,151,691 - Psyr_1019 0.22 -0.4 1,151,691 - Psyr_1019 0.22 -0.8 1,151,691 - Psyr_1019 0.22 -0.2 1,151,691 - Psyr_1019 0.22 -1.5 1,151,805 - Psyr_1019 0.52 +0.2 1,151,805 - Psyr_1019 0.52 -1.2 1,151,856 - Psyr_1019 0.66 -0.9 1,151,856 - Psyr_1019 0.66 +2.3 1,151,983 + -0.0 1,152,065 + Psyr_1020 0.15 +1.3 1,152,065 + Psyr_1020 0.15 -0.1 1,152,065 + Psyr_1020 0.15 -1.6 1,152,065 + Psyr_1020 0.15 +0.6 1,152,065 + Psyr_1020 0.15 -1.5 1,152,065 + Psyr_1020 0.15 -0.8 1,152,065 + Psyr_1020 0.15 +2.0 1,152,066 - Psyr_1020 0.15 -1.4 1,152,066 - Psyr_1020 0.15 -0.0 1,152,066 - Psyr_1020 0.15 -1.6 1,152,066 - Psyr_1020 0.15 +0.4 1,152,066 - Psyr_1020 0.15 +2.0 1,152,066 - Psyr_1020 0.15 +2.0 1,152,066 - Psyr_1020 0.15 -1.7 1,152,066 - Psyr_1020 0.15 +0.5 1,152,066 - Psyr_1020 0.15 -0.8 1,152,066 - Psyr_1020 0.15 -0.6 1,152,066 - Psyr_1020 0.15 -0.5 1,152,066 - Psyr_1020 0.15 -0.0 1,152,139 - Psyr_1020 0.28 +1.2 1,152,139 - Psyr_1020 0.28 +0.0
Or see this region's nucleotide sequence