Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0578 and Psyr_RNA2 are separated by 106 nucleotides Psyr_RNA2 and Psyr_RNA3 are separated by 160 nucleotides Psyr_RNA3 and Psyr_0579 are separated by 225 nucleotides Psyr_0579 and Psyr_0580 overlap by 4 nucleotides
Psyr_0578: Psyr_0578 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 657,338 to 659,272
_0578
Psyr_RNA2: Psyr_RNA2 - tRNA-Leu, at 659,379 to 659,465
_RNA2
Psyr_RNA3: Psyr_RNA3 - tRNA-Leu, at 659,626 to 659,712
_RNA3
Psyr_0579: Psyr_0579 - RNAse R, at 659,938 to 662,571
_0579
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Position (kb) 659
660
661
662
663 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 659.019 kb on + strand, within Psyr_0578 at 659.020 kb on - strand, within Psyr_0578 at 659.482 kb on - strand at 659.502 kb on + strand at 659.502 kb on + strand at 659.504 kb on + strand at 659.505 kb on - strand at 659.505 kb on - strand at 659.525 kb on + strand at 659.543 kb on + strand at 659.543 kb on + strand at 659.543 kb on + strand at 659.544 kb on - strand at 659.547 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.615 kb on - strand at 659.615 kb on - strand at 659.615 kb on - strand at 659.750 kb on - strand at 659.751 kb on + strand at 659.751 kb on + strand at 659.752 kb on - strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.765 kb on + strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.766 kb on - strand at 659.895 kb on + strand at 659.895 kb on + strand at 659.895 kb on + strand at 659.896 kb on - strand at 659.896 kb on - strand at 659.906 kb on - strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.913 kb on - strand at 659.913 kb on - strand at 659.913 kb on - strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.936 kb on + strand at 659.936 kb on + strand at 659.936 kb on + strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.158 kb on - strand at 660.175 kb on + strand at 660.175 kb on + strand at 660.176 kb on - strand at 660.176 kb on - strand at 660.176 kb on - strand at 660.404 kb on + strand, within Psyr_0579 at 660.405 kb on - strand, within Psyr_0579 at 660.415 kb on + strand, within Psyr_0579 at 660.415 kb on + strand, within Psyr_0579 at 660.415 kb on + strand, within Psyr_0579 at 660.415 kb on + strand, within Psyr_0579 at 660.416 kb on - strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.636 kb on + strand, within Psyr_0579 at 660.636 kb on + strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.640 kb on + strand, within Psyr_0579 at 660.854 kb on - strand, within Psyr_0579 at 660.854 kb on - strand, within Psyr_0579 at 660.873 kb on - strand, within Psyr_0579 at 660.880 kb on + strand, within Psyr_0579 at 660.881 kb on - strand, within Psyr_0579 at 660.937 kb on + strand, within Psyr_0579 at 660.938 kb on - strand, within Psyr_0579 at 661.070 kb on - strand, within Psyr_0579 at 661.078 kb on + strand, within Psyr_0579 at 661.079 kb on - strand, within Psyr_0579 at 661.114 kb on + strand, within Psyr_0579 at 661.115 kb on - strand, within Psyr_0579 at 661.115 kb on - strand, within Psyr_0579 at 661.213 kb on + strand, within Psyr_0579 at 661.213 kb on + strand, within Psyr_0579 at 661.213 kb on + strand, within Psyr_0579 at 661.214 kb on - strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.229 kb on + strand, within Psyr_0579 at 661.229 kb on + strand, within Psyr_0579 at 661.229 kb on + strand, within Psyr_0579 at 661.230 kb on - strand, within Psyr_0579 at 661.339 kb on - strand, within Psyr_0579 at 661.429 kb on + strand, within Psyr_0579 at 661.430 kb on - strand, within Psyr_0579 at 661.579 kb on + strand, within Psyr_0579 at 661.580 kb on - strand, within Psyr_0579 at 661.628 kb on - strand, within Psyr_0579 at 661.663 kb on + strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.811 kb on - strand, within Psyr_0579 at 661.895 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.968 kb on - strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.047 kb on + strand, within Psyr_0579 at 662.048 kb on - strand, within Psyr_0579 at 662.051 kb on - strand, within Psyr_0579 at 662.227 kb on - strand, within Psyr_0579 at 662.421 kb on + strand at 662.422 kb on - strand at 662.550 kb on + strand at 662.605 kb on + strand at 662.605 kb on + strand at 662.606 kb on - strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.615 kb on - strand at 662.915 kb on + strand, within Psyr_0580 at 662.915 kb on + strand, within Psyr_0580 at 663.051 kb on - strand, within Psyr_0580 at 663.121 kb on - strand, within Psyr_0580 at 663.152 kb on + strand, within Psyr_0580 at 663.207 kb on + strand, within Psyr_0580 at 663.208 kb on - strand, within Psyr_0580 at 663.321 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.422 kb on - strand at 663.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 659,019 + Psyr_0578 0.87 -0.0 659,020 - Psyr_0578 0.87 +1.0 659,482 - -0.2 659,502 + +0.8 659,502 + +0.1 659,504 + -0.7 659,505 - -0.4 659,505 - +1.7 659,525 + +1.2 659,543 + +0.9 659,543 + -0.9 659,543 + -0.2 659,544 - -0.8 659,547 - +3.3 659,610 - -0.3 659,610 - -0.7 659,610 - +0.3 659,610 - +0.0 659,615 - -0.0 659,615 - +1.4 659,615 - -1.2 659,750 - +1.3 659,751 + +1.2 659,751 + +0.1 659,752 - -0.7 659,765 + +1.0 659,765 + +1.1 659,765 + -0.6 659,765 + -1.0 659,766 - +1.6 659,766 - -0.6 659,766 - -2.0 659,766 - +2.6 659,895 + +0.9 659,895 + +0.0 659,895 + +0.1 659,896 - -1.2 659,896 - -3.3 659,906 - -1.0 659,912 + +0.4 659,912 + -0.3 659,912 + -0.6 659,913 - -3.2 659,913 - +2.0 659,913 - -0.0 659,914 + +1.0 659,914 + +1.2 659,914 + +0.8 659,914 + +1.0 659,914 + +0.7 659,914 + +0.1 659,914 + -2.2 659,915 - -2.5 659,915 - -1.8 659,915 - -2.8 659,916 + +1.4 659,916 + -1.7 659,916 + +0.8 659,916 + +1.9 659,916 + +0.7 659,916 + +0.0 659,916 + +0.4 659,916 + -1.2 659,916 + -0.4 659,916 + +0.3 659,916 + -0.8 659,916 + -0.1 659,916 + +0.1 659,916 + +0.3 659,916 + -0.5 659,916 + +1.0 659,916 + +0.7 659,916 + +0.7 659,916 + -1.4 659,916 + -0.1 659,916 + -0.4 659,916 + +0.4 659,917 - -2.6 659,917 - -1.5 659,917 - +2.0 659,917 - -2.2 659,917 - +1.0 659,917 - -2.3 659,917 - -1.3 659,917 - -3.3 659,917 - +2.6 659,917 - -2.6 659,917 - +1.0 659,917 - -1.3 659,917 - -1.0 659,917 - -0.4 659,917 - -3.0 659,917 - -1.7 659,917 - -1.1 659,918 + +0.6 659,918 + -0.1 659,918 + +0.4 659,918 + +0.1 659,918 + -1.8 659,918 + -0.8 659,918 + -0.9 659,918 + +0.6 659,918 + -1.6 659,918 + -0.4 659,918 + -0.6 659,918 + -2.0 659,918 + +0.4 659,918 + -0.3 659,918 + +3.0 659,918 + +1.0 659,918 + -0.5 659,919 - -2.5 659,919 - -1.3 659,919 - -1.0 659,919 - -1.6 659,919 - -2.7 659,919 - -2.0 659,919 - -1.3 659,919 - -2.0 659,919 - -0.6 659,936 + +0.6 659,936 + -1.0 659,936 + -0.9 660,158 - -1.1 660,158 - -1.7 660,158 - -0.3 660,158 - -1.8 660,175 + -0.6 660,175 + -2.3 660,176 - +0.6 660,176 - +0.5 660,176 - -1.0 660,404 + Psyr_0579 0.18 -0.6 660,405 - Psyr_0579 0.18 +2.0 660,415 + Psyr_0579 0.18 -1.0 660,415 + Psyr_0579 0.18 -1.2 660,415 + Psyr_0579 0.18 -1.0 660,415 + Psyr_0579 0.18 -0.8 660,416 - Psyr_0579 0.18 -1.6 660,598 + Psyr_0579 0.25 +0.4 660,598 + Psyr_0579 0.25 -0.6 660,598 + Psyr_0579 0.25 -1.4 660,598 + Psyr_0579 0.25 -2.8 660,598 + Psyr_0579 0.25 -2.0 660,599 - Psyr_0579 0.25 -1.8 660,599 - Psyr_0579 0.25 +0.1 660,599 - Psyr_0579 0.25 -0.0 660,599 - Psyr_0579 0.25 -1.0 660,599 - Psyr_0579 0.25 -1.2 660,599 - Psyr_0579 0.25 -1.8 660,636 + Psyr_0579 0.26 -0.1 660,636 + Psyr_0579 0.26 +1.6 660,637 - Psyr_0579 0.27 -3.4 660,637 - Psyr_0579 0.27 -0.8 660,637 - Psyr_0579 0.27 -2.2 660,637 - Psyr_0579 0.27 +1.0 660,637 - Psyr_0579 0.27 -0.6 660,637 - Psyr_0579 0.27 -0.3 660,637 - Psyr_0579 0.27 -0.8 660,640 + Psyr_0579 0.27 -1.2 660,854 - Psyr_0579 0.35 -2.4 660,854 - Psyr_0579 0.35 -2.6 660,873 - Psyr_0579 0.35 -1.4 660,880 + Psyr_0579 0.36 -0.4 660,881 - Psyr_0579 0.36 -0.2 660,937 + Psyr_0579 0.38 -2.2 660,938 - Psyr_0579 0.38 -2.5 661,070 - Psyr_0579 0.43 -1.3 661,078 + Psyr_0579 0.43 -1.3 661,079 - Psyr_0579 0.43 -1.6 661,114 + Psyr_0579 0.45 -2.6 661,115 - Psyr_0579 0.45 -4.2 661,115 - Psyr_0579 0.45 +2.0 661,213 + Psyr_0579 0.48 -3.8 661,213 + Psyr_0579 0.48 -1.5 661,213 + Psyr_0579 0.48 -1.0 661,214 - Psyr_0579 0.48 -1.1 661,222 + Psyr_0579 0.49 -2.6 661,222 + Psyr_0579 0.49 -1.3 661,222 + Psyr_0579 0.49 -2.6 661,222 + Psyr_0579 0.49 +1.0 661,222 + Psyr_0579 0.49 +0.4 661,223 - Psyr_0579 0.49 -2.0 661,223 - Psyr_0579 0.49 +0.4 661,223 - Psyr_0579 0.49 -1.6 661,223 - Psyr_0579 0.49 +2.6 661,223 - Psyr_0579 0.49 -1.6 661,229 + Psyr_0579 0.49 -0.0 661,229 + Psyr_0579 0.49 -1.8 661,229 + Psyr_0579 0.49 -1.8 661,230 - Psyr_0579 0.49 -0.9 661,339 - Psyr_0579 0.53 -0.9 661,429 + Psyr_0579 0.57 +0.2 661,430 - Psyr_0579 0.57 -3.2 661,579 + Psyr_0579 0.62 -1.3 661,580 - Psyr_0579 0.62 -2.5 661,628 - Psyr_0579 0.64 -2.2 661,663 + Psyr_0579 0.65 -2.0 661,672 + Psyr_0579 0.66 -1.2 661,811 - Psyr_0579 0.71 -1.0 661,895 + Psyr_0579 0.74 -1.3 661,918 + Psyr_0579 0.75 -1.1 661,918 + Psyr_0579 0.75 -0.5 661,919 - Psyr_0579 0.75 -0.0 661,919 - Psyr_0579 0.75 -1.8 661,919 - Psyr_0579 0.75 -0.8 661,968 - Psyr_0579 0.77 -2.6 662,005 + Psyr_0579 0.78 -0.6 662,005 + Psyr_0579 0.78 -1.6 662,005 + Psyr_0579 0.78 -3.0 662,005 + Psyr_0579 0.78 -0.2 662,005 + Psyr_0579 0.78 -2.6 662,005 + Psyr_0579 0.78 -1.6 662,005 + Psyr_0579 0.78 -1.6 662,005 + Psyr_0579 0.78 +2.0 662,005 + Psyr_0579 0.78 -0.5 662,005 + Psyr_0579 0.78 -0.6 662,005 + Psyr_0579 0.78 -0.3 662,006 - Psyr_0579 0.79 -0.8 662,006 - Psyr_0579 0.79 -0.9 662,006 - Psyr_0579 0.79 -1.3 662,006 - Psyr_0579 0.79 -0.9 662,006 - Psyr_0579 0.79 +1.0 662,006 - Psyr_0579 0.79 -1.8 662,006 - Psyr_0579 0.79 -2.9 662,006 - Psyr_0579 0.79 -2.2 662,006 - Psyr_0579 0.79 -1.6 662,006 - Psyr_0579 0.79 -1.6 662,006 - Psyr_0579 0.79 -2.4 662,006 - Psyr_0579 0.79 -1.7 662,006 - Psyr_0579 0.79 +0.1 662,006 - Psyr_0579 0.79 -1.7 662,006 - Psyr_0579 0.79 -1.7 662,047 + Psyr_0579 0.80 -0.7 662,048 - Psyr_0579 0.80 +0.8 662,051 - Psyr_0579 0.80 -2.2 662,227 - Psyr_0579 0.87 +1.3 662,421 + +0.4 662,422 - +0.2 662,550 + +0.1 662,605 + -0.6 662,605 + -1.0 662,606 - +1.0 662,614 + +1.1 662,614 + +2.4 662,614 + -0.4 662,615 - -0.6 662,915 + Psyr_0580 0.46 -0.7 662,915 + Psyr_0580 0.46 -1.7 663,051 - Psyr_0580 0.64 -0.2 663,121 - Psyr_0580 0.73 -1.3 663,152 + Psyr_0580 0.77 -1.9 663,207 + Psyr_0580 0.85 -1.5 663,208 - Psyr_0580 0.85 +0.1 663,321 + -3.1 663,324 + -0.5 663,324 + +0.1 663,324 + +0.7 663,324 + +0.4 663,352 + +0.3 663,352 + -2.7 663,352 + +0.5 663,352 + -0.7 663,352 + -0.0 663,352 + -0.2 663,353 - -0.1 663,353 - -0.0 663,353 - -0.5 663,353 - +0.8 663,359 + -0.7 663,359 + -0.7 663,359 + +0.7 663,359 + -0.0 663,360 - +1.1 663,360 - -0.6 663,360 - +2.0 663,360 - +0.2 663,360 - -0.0 663,360 - -1.5 663,360 - +2.0 663,376 + -0.4 663,376 + -0.8 663,377 - +0.3 663,377 - +0.8 663,377 - -0.6 663,377 - -2.3 663,377 - +0.1 663,382 + +0.7 663,383 - -0.5 663,383 - -0.7 663,421 + +0.2 663,422 - +1.1 663,422 - +1.0
Or see this region's nucleotide sequence