Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0474 and Psyr_0475 are separated by 266 nucleotides Psyr_0475 and Psyr_0476 are separated by 25 nucleotides Psyr_0476 and Psyr_0477 are separated by 21 nucleotides
Psyr_0474: Psyr_0474 - homoserine O-acetyltransferase, at 512,859 to 513,998
_0474
Psyr_0475: Psyr_0475 - Protein of unknown function YGGT, at 514,265 to 514,855
_0475
Psyr_0476: Psyr_0476 - pyrroline-5-carboxylate reductase, at 514,881 to 515,699
_0476
Psyr_0477: Psyr_0477 - Protein of unknown function UPF0001, at 515,721 to 516,407
_0477
Position (kb) 514
515 Strain fitness (log2 ratio) -2
-1
0
1
2 at 513.298 kb on + strand, within Psyr_0474 at 513.298 kb on + strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.723 kb on - strand, within Psyr_0474 at 513.874 kb on + strand, within Psyr_0474 at 513.874 kb on + strand, within Psyr_0474 at 513.875 kb on - strand, within Psyr_0474 at 513.875 kb on - strand, within Psyr_0474 at 513.883 kb on + strand, within Psyr_0474 at 513.883 kb on + strand, within Psyr_0474 at 513.895 kb on + strand at 513.895 kb on + strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.988 kb on - strand at 514.015 kb on + strand at 514.016 kb on - strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.058 kb on - strand at 514.064 kb on + strand at 514.064 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.093 kb on - strand at 514.093 kb on - strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.097 kb on - strand at 514.097 kb on - strand at 514.107 kb on + strand at 514.107 kb on + strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.146 kb on + strand at 514.147 kb on - strand at 514.147 kb on - strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.167 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.303 kb on + strand at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.788 kb on - strand, within Psyr_0475 at 514.800 kb on + strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 515.167 kb on + strand, within Psyr_0476 at 515.496 kb on + strand, within Psyr_0476 at 515.734 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 513,298 + Psyr_0474 0.39 -0.3 513,298 + Psyr_0474 0.39 +0.2 513,299 - Psyr_0474 0.39 -0.2 513,299 - Psyr_0474 0.39 -0.6 513,299 - Psyr_0474 0.39 +0.1 513,299 - Psyr_0474 0.39 -1.2 513,723 - Psyr_0474 0.76 -0.2 513,874 + Psyr_0474 0.89 -0.9 513,874 + Psyr_0474 0.89 +0.6 513,875 - Psyr_0474 0.89 -0.1 513,875 - Psyr_0474 0.89 +0.5 513,883 + Psyr_0474 0.90 -1.6 513,883 + Psyr_0474 0.90 +1.0 513,895 + -1.0 513,895 + -0.3 513,896 - +0.6 513,896 - -0.3 513,896 - +2.0 513,988 - +0.4 514,015 + +0.7 514,016 - -0.3 514,026 + +1.4 514,026 + +1.2 514,026 + +1.3 514,026 + -0.6 514,026 + -0.2 514,026 + -0.3 514,026 + +0.9 514,027 - +0.5 514,027 - +0.2 514,027 - +1.0 514,032 + +0.4 514,032 + +0.0 514,058 - -1.4 514,064 + -0.8 514,064 + -0.1 514,092 + +0.0 514,092 + +0.1 514,092 + +0.6 514,092 + -0.2 514,093 - -1.4 514,093 - -0.3 514,096 + -0.3 514,096 + +0.2 514,096 + +0.7 514,097 - -1.3 514,097 - -0.7 514,107 + +1.4 514,107 + -0.3 514,108 - -0.0 514,108 - -0.2 514,108 - +0.1 514,108 - +0.9 514,108 - -0.9 514,146 + +0.8 514,147 - -0.1 514,147 - -0.8 514,148 + +0.4 514,148 + -0.2 514,148 + +0.0 514,148 + +0.6 514,148 + -1.1 514,149 - -1.2 514,149 - +0.6 514,149 - -0.7 514,149 - -1.1 514,149 - +0.3 514,163 + -0.8 514,163 + +2.0 514,163 + +0.2 514,163 + +0.9 514,163 + +0.2 514,163 + -0.2 514,163 + +1.7 514,163 + +1.3 514,164 - +0.4 514,164 - +0.5 514,164 - -0.3 514,164 - -1.8 514,164 - +1.0 514,164 - +0.1 514,164 - -0.0 514,164 - +0.2 514,164 - +0.4 514,164 - +2.0 514,164 - -0.2 514,164 - -0.2 514,164 - +1.7 514,164 - -0.3 514,164 - -1.1 514,167 + -0.1 514,266 + +1.2 514,266 + -1.1 514,266 + -1.5 514,266 + +1.9 514,266 + -0.1 514,266 + +0.4 514,266 + +0.0 514,266 + +1.2 514,266 + -0.8 514,266 + -1.0 514,266 + +0.4 514,266 + +0.1 514,266 + +0.0 514,267 - +1.3 514,267 - -0.6 514,267 - +0.3 514,267 - -0.2 514,267 - -0.6 514,267 - +0.1 514,267 - -0.3 514,267 - +0.3 514,267 - -0.1 514,267 - -0.5 514,267 - +0.7 514,267 - -0.4 514,303 + -0.2 514,746 + Psyr_0475 0.81 +1.0 514,746 + Psyr_0475 0.81 -1.3 514,746 + Psyr_0475 0.81 -1.8 514,746 + Psyr_0475 0.81 -1.6 514,746 + Psyr_0475 0.81 -1.7 514,746 + Psyr_0475 0.81 +0.0 514,787 + Psyr_0475 0.88 -1.3 514,787 + Psyr_0475 0.88 -1.6 514,787 + Psyr_0475 0.88 -1.7 514,788 - Psyr_0475 0.88 +0.2 514,800 + +0.2 514,828 - -0.8 514,828 - -2.7 514,828 - -1.2 514,828 - -0.6 514,828 - -1.0 515,167 + Psyr_0476 0.35 +1.7 515,496 + Psyr_0476 0.75 -1.0 515,734 + -0.6
Or see this region's nucleotide sequence