Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0262 and Psyr_0263 are separated by 197 nucleotides Psyr_0263 and Psyr_0264 are separated by 10 nucleotides
Psyr_0262: Psyr_0262 - Transport-associated protein, at 277,811 to 278,650
_0262
Psyr_0263: Psyr_0263 - Two-component response regulator AlgB, at 278,848 to 280,194
_0263
Psyr_0264: Psyr_0264 - Alginate biosynthesis sensor protein KinB, at 280,205 to 281,992
_0264
Position (kb) 278
279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 277.874 kb on - strand at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 278.650 kb on + strand at 278.651 kb on - strand at 278.706 kb on + strand at 278.706 kb on + strand at 278.736 kb on + strand at 278.737 kb on - strand at 278.753 kb on - strand at 278.753 kb on - strand at 278.814 kb on + strand at 278.814 kb on + strand at 278.840 kb on + strand at 278.944 kb on + strand at 278.945 kb on - strand at 278.945 kb on - strand at 278.945 kb on - strand at 279.002 kb on + strand, within Psyr_0263 at 279.003 kb on - strand, within Psyr_0263 at 279.163 kb on + strand, within Psyr_0263 at 279.164 kb on - strand, within Psyr_0263 at 279.650 kb on - strand, within Psyr_0263 at 279.763 kb on + strand, within Psyr_0263 at 279.764 kb on - strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.919 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.996 kb on - strand, within Psyr_0263 at 280.100 kb on + strand at 280.100 kb on + strand at 280.101 kb on - strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.191 kb on - strand at 280.191 kb on - strand at 280.197 kb on + strand at 280.197 kb on + strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.319 kb on - strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.538 kb on + strand, within Psyr_0264 at 280.539 kb on - strand, within Psyr_0264 at 280.568 kb on + strand, within Psyr_0264 at 280.569 kb on - strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 277,874 - +1.5 277,992 + Psyr_0262 0.22 -0.1 277,992 + Psyr_0262 0.22 +1.5 277,992 + Psyr_0262 0.22 +0.8 277,992 + Psyr_0262 0.22 +0.4 277,992 + Psyr_0262 0.22 -1.3 277,992 + Psyr_0262 0.22 +1.8 277,992 + Psyr_0262 0.22 -0.3 277,993 - Psyr_0262 0.22 -0.2 277,993 - Psyr_0262 0.22 +0.5 277,993 - Psyr_0262 0.22 -0.6 277,993 - Psyr_0262 0.22 -0.2 277,993 - Psyr_0262 0.22 -0.0 278,650 + -0.1 278,651 - +0.7 278,706 + -0.0 278,706 + -0.0 278,736 + -2.9 278,737 - -0.5 278,753 - +0.1 278,753 - +0.1 278,814 + +0.9 278,814 + -0.1 278,840 + +0.2 278,944 + -1.2 278,945 - -0.4 278,945 - -0.7 278,945 - +1.0 279,002 + Psyr_0263 0.11 -0.9 279,003 - Psyr_0263 0.12 -0.2 279,163 + Psyr_0263 0.23 -1.1 279,164 - Psyr_0263 0.23 -1.8 279,650 - Psyr_0263 0.60 +0.2 279,763 + Psyr_0263 0.68 -0.5 279,764 - Psyr_0263 0.68 -0.8 279,865 + Psyr_0263 0.76 -1.7 279,865 + Psyr_0263 0.76 -0.6 279,865 + Psyr_0263 0.76 +1.6 279,866 - Psyr_0263 0.76 -2.3 279,866 - Psyr_0263 0.76 -3.3 279,919 + Psyr_0263 0.80 +0.4 279,995 + Psyr_0263 0.85 -1.5 279,995 + Psyr_0263 0.85 +0.7 279,996 - Psyr_0263 0.85 -0.1 280,100 + -0.3 280,100 + +0.5 280,101 - -1.1 280,183 + -1.0 280,183 + -0.8 280,183 + -0.0 280,184 - -1.4 280,184 - -0.3 280,184 - -1.1 280,190 + -0.4 280,190 + -0.5 280,190 + -1.8 280,190 + +0.0 280,191 - +1.6 280,191 - +0.0 280,197 + -0.6 280,197 + -0.5 280,198 - +1.0 280,198 - -1.0 280,198 - -0.2 280,198 - +0.5 280,198 - -0.6 280,319 - +0.2 280,342 + +0.1 280,342 + +1.4 280,342 + +0.2 280,342 + +1.0 280,538 + Psyr_0264 0.19 +0.0 280,539 - Psyr_0264 0.19 -1.3 280,568 + Psyr_0264 0.20 -0.1 280,569 - Psyr_0264 0.20 -1.2 280,590 + Psyr_0264 0.22 -0.6 280,590 + Psyr_0264 0.22 -1.2 280,591 - Psyr_0264 0.22 -0.5 280,591 - Psyr_0264 0.22 +0.0 280,591 - Psyr_0264 0.22 +1.8 281,003 - Psyr_0264 0.45 +1.6 281,003 - Psyr_0264 0.45 -0.7
Or see this region's nucleotide sequence