Experiment: KB with Avocatin B 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0080 and Psyr_0081 are separated by 200 nucleotides Psyr_0081 and Psyr_0082 are separated by 2 nucleotides Psyr_0082 and Psyr_0083 are separated by 13 nucleotides
Psyr_0080: Psyr_0080 - Protein of unknown function DUF81, at 86,468 to 87,280
_0080
Psyr_0081: Psyr_0081 - ABC transporter, at 87,481 to 88,461
_0081
Psyr_0082: Psyr_0082 - Binding-protein-dependent transport systems inner membrane component, at 88,464 to 89,336
_0082
Psyr_0083: Psyr_0083 - Binding-protein-dependent transport systems inner membrane component, at 89,350 to 90,171
_0083
Position (kb) 87
88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 86.520 kb on + strand at 86.697 kb on - strand, within Psyr_0080 at 86.697 kb on - strand, within Psyr_0080 at 86.697 kb on - strand, within Psyr_0080 at 86.715 kb on + strand, within Psyr_0080 at 86.715 kb on + strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.798 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 87.121 kb on + strand, within Psyr_0080 at 87.121 kb on + strand, within Psyr_0080 at 87.122 kb on - strand, within Psyr_0080 at 87.341 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.459 kb on - strand at 87.483 kb on - strand at 87.483 kb on - strand at 87.788 kb on + strand, within Psyr_0081 at 87.789 kb on - strand, within Psyr_0081 at 88.465 kb on + strand at 88.465 kb on + strand at 88.466 kb on - strand at 88.482 kb on - strand at 88.588 kb on + strand, within Psyr_0082 at 88.588 kb on + strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.589 kb on - strand, within Psyr_0082 at 88.695 kb on - strand, within Psyr_0082 at 88.712 kb on - strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.963 kb on + strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 88.964 kb on - strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.044 kb on + strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.045 kb on - strand, within Psyr_0082 at 89.239 kb on - strand, within Psyr_0082 at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.329 kb on + strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.330 kb on - strand at 89.450 kb on + strand, within Psyr_0083 at 89.450 kb on + strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083 at 89.451 kb on - strand, within Psyr_0083
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM remove 86,520 + +2.1 86,697 - Psyr_0080 0.28 +0.4 86,697 - Psyr_0080 0.28 -1.6 86,697 - Psyr_0080 0.28 +1.2 86,715 + Psyr_0080 0.30 +0.3 86,715 + Psyr_0080 0.30 -0.1 86,716 - Psyr_0080 0.31 -0.4 86,716 - Psyr_0080 0.31 +1.1 86,716 - Psyr_0080 0.31 -0.5 86,798 + Psyr_0080 0.41 +1.8 86,812 + Psyr_0080 0.42 +0.5 86,812 + Psyr_0080 0.42 -1.7 86,812 + Psyr_0080 0.42 -0.6 86,812 + Psyr_0080 0.42 +0.3 86,812 + Psyr_0080 0.42 -1.1 86,812 + Psyr_0080 0.42 +1.0 86,812 + Psyr_0080 0.42 -0.1 86,812 + Psyr_0080 0.42 +1.2 86,812 + Psyr_0080 0.42 +0.4 86,812 + Psyr_0080 0.42 -0.8 86,812 + Psyr_0080 0.42 +0.7 86,812 + Psyr_0080 0.42 +0.9 86,812 + Psyr_0080 0.42 -0.3 86,812 + Psyr_0080 0.42 -1.0 86,812 + Psyr_0080 0.42 -0.7 86,812 + Psyr_0080 0.42 -1.0 86,812 + Psyr_0080 0.42 -1.2 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 +0.3 86,813 - Psyr_0080 0.42 -0.7 86,813 - Psyr_0080 0.42 +0.8 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 +0.9 86,813 - Psyr_0080 0.42 +0.2 86,813 - Psyr_0080 0.42 +0.5 86,813 - Psyr_0080 0.42 +0.1 86,813 - Psyr_0080 0.42 +1.8 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 -0.7 86,813 - Psyr_0080 0.42 -0.8 86,813 - Psyr_0080 0.42 -0.2 86,813 - Psyr_0080 0.42 +0.4 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 -0.2 86,813 - Psyr_0080 0.42 +0.8 86,813 - Psyr_0080 0.42 -0.8 86,813 - Psyr_0080 0.42 -0.2 86,813 - Psyr_0080 0.42 +2.7 86,813 - Psyr_0080 0.42 -0.2 86,813 - Psyr_0080 0.42 -0.0 86,813 - Psyr_0080 0.42 -0.1 86,813 - Psyr_0080 0.42 -0.0 86,813 - Psyr_0080 0.42 +0.5 86,813 - Psyr_0080 0.42 -1.0 86,871 + Psyr_0080 0.50 +0.7 86,871 + Psyr_0080 0.50 -0.5 86,871 + Psyr_0080 0.50 +1.3 86,871 + Psyr_0080 0.50 -0.5 86,871 + Psyr_0080 0.50 +0.1 87,121 + Psyr_0080 0.80 -0.3 87,121 + Psyr_0080 0.80 +0.5 87,122 - Psyr_0080 0.80 -1.3 87,341 + +0.3 87,456 + +0.9 87,456 + -0.7 87,456 + -1.5 87,456 + -1.0 87,456 + -0.3 87,456 + -0.6 87,456 + +0.1 87,456 + -0.2 87,456 + -0.5 87,456 + -1.4 87,457 - -0.1 87,457 - +0.3 87,457 - -1.6 87,457 - -0.2 87,457 - -2.1 87,457 - +0.2 87,457 - +0.3 87,459 - +0.7 87,483 - +0.9 87,483 - +0.1 87,788 + Psyr_0081 0.31 -1.5 87,789 - Psyr_0081 0.31 -0.6 88,465 + -0.1 88,465 + -0.7 88,466 - +1.2 88,482 - +1.0 88,588 + Psyr_0082 0.14 +0.3 88,588 + Psyr_0082 0.14 +0.6 88,589 - Psyr_0082 0.14 +0.5 88,589 - Psyr_0082 0.14 -0.1 88,589 - Psyr_0082 0.14 -0.5 88,589 - Psyr_0082 0.14 +0.1 88,695 - Psyr_0082 0.26 -0.9 88,712 - Psyr_0082 0.28 -0.1 88,963 + Psyr_0082 0.57 +0.1 88,963 + Psyr_0082 0.57 -0.6 88,963 + Psyr_0082 0.57 -3.2 88,964 - Psyr_0082 0.57 -0.1 88,964 - Psyr_0082 0.57 +0.3 88,964 - Psyr_0082 0.57 +0.6 88,964 - Psyr_0082 0.57 -0.6 89,044 + Psyr_0082 0.66 +0.3 89,044 + Psyr_0082 0.66 -1.2 89,045 - Psyr_0082 0.67 -0.1 89,045 - Psyr_0082 0.67 -0.5 89,239 - Psyr_0082 0.89 -0.2 89,329 + +0.0 89,329 + -0.4 89,329 + -1.7 89,329 + +0.4 89,330 - -0.0 89,330 - -0.0 89,330 - +0.4 89,450 + Psyr_0083 0.12 +3.4 89,450 + Psyr_0083 0.12 +0.6 89,451 - Psyr_0083 0.12 +1.4 89,451 - Psyr_0083 0.12 +2.1
Or see this region's nucleotide sequence