Experiment: BHIS with 2-Mercaptopyridine N-oxide 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0007 and BT0008 are separated by 108 nucleotides BT0008 and BT0009 are separated by 128 nucleotides BT0009 and BT0010 are separated by 54 nucleotides
BT0007: BT0007 - hypothetical protein (NCBI ptt file), at 4,457 to 4,849
BT0007
BT0008: BT0008 - conserved hypothetical protein (NCBI ptt file), at 4,958 to 5,503
BT0008
BT0009: BT0009 - phosphate-binding periplasmic protein precursor, putative ABC transporter (NCBI ptt file), at 5,632 to 6,741
BT0009
BT0010: BT0010 - hypothetical protein (NCBI ptt file), at 6,796 to 7,869
BT0010
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4.714 kb on - strand, within BT0007 at 4.714 kb on - strand, within BT0007 at 4.747 kb on + strand, within BT0007 at 4.969 kb on - strand at 5.078 kb on + strand, within BT0008 at 5.101 kb on + strand, within BT0008 at 5.105 kb on + strand, within BT0008 at 5.280 kb on + strand, within BT0008 at 5.296 kb on + strand, within BT0008 at 5.297 kb on - strand, within BT0008 at 5.350 kb on + strand, within BT0008 at 5.368 kb on + strand, within BT0008 at 5.475 kb on + strand at 5.480 kb on + strand at 5.519 kb on + strand at 5.520 kb on - strand at 5.631 kb on + strand at 5.643 kb on + strand at 5.676 kb on - strand at 5.676 kb on - strand at 5.721 kb on - strand at 5.725 kb on - strand at 5.868 kb on + strand, within BT0009 at 5.925 kb on - strand, within BT0009 at 5.925 kb on - strand, within BT0009 at 5.989 kb on - strand, within BT0009 at 6.001 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.218 kb on - strand, within BT0009 at 6.516 kb on + strand, within BT0009 at 6.540 kb on - strand, within BT0009 at 6.545 kb on - strand, within BT0009 at 6.546 kb on + strand, within BT0009 at 6.716 kb on - strand at 6.716 kb on - strand at 6.744 kb on + strand at 6.750 kb on + strand at 6.751 kb on - strand at 6.758 kb on + strand at 6.759 kb on - strand at 6.834 kb on + strand at 6.858 kb on - strand at 6.858 kb on - strand at 6.925 kb on - strand, within BT0010 at 7.277 kb on + strand, within BT0010 at 7.277 kb on + strand, within BT0010 at 7.348 kb on - strand, within BT0010 at 7.348 kb on - strand, within BT0010 at 7.353 kb on - strand, within BT0010 at 7.614 kb on - strand, within BT0010 at 7.627 kb on - strand, within BT0010
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 2-Mercaptopyridine N-oxide 0.2 mM remove 4,714 - BT0007 0.65 +1.1 4,714 - BT0007 0.65 +0.1 4,747 + BT0007 0.74 -0.9 4,969 - -0.9 5,078 + BT0008 0.22 -0.8 5,101 + BT0008 0.26 +0.2 5,105 + BT0008 0.27 -1.5 5,280 + BT0008 0.59 -2.5 5,296 + BT0008 0.62 -2.6 5,297 - BT0008 0.62 +1.1 5,350 + BT0008 0.72 -1.5 5,368 + BT0008 0.75 -0.9 5,475 + -0.5 5,480 + +0.1 5,519 + -1.1 5,520 - -0.9 5,631 + +0.1 5,643 + -2.2 5,676 - -1.2 5,676 - -1.2 5,721 - -1.1 5,725 - -0.3 5,868 + BT0009 0.21 -1.0 5,925 - BT0009 0.26 -1.6 5,925 - BT0009 0.26 +0.7 5,989 - BT0009 0.32 +0.1 6,001 - BT0009 0.33 -2.4 6,086 - BT0009 0.41 -0.9 6,086 - BT0009 0.41 -0.9 6,086 - BT0009 0.41 +0.1 6,218 - BT0009 0.53 -0.8 6,516 + BT0009 0.80 -2.2 6,540 - BT0009 0.82 -1.6 6,545 - BT0009 0.82 -1.1 6,546 + BT0009 0.82 -0.9 6,716 - -0.1 6,716 - -0.9 6,744 + +0.1 6,750 + +0.9 6,751 - +2.1 6,758 + -3.4 6,759 - -1.0 6,834 + +1.1 6,858 - +1.1 6,858 - -0.7 6,925 - BT0010 0.12 +0.1 7,277 + BT0010 0.45 +0.1 7,277 + BT0010 0.45 +0.1 7,348 - BT0010 0.51 +0.1 7,348 - BT0010 0.51 +0.1 7,353 - BT0010 0.52 -1.5 7,614 - BT0010 0.76 +2.4 7,627 - BT0010 0.77 -2.5
Or see this region's nucleotide sequence