Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07075 and MMP_RS07080 are separated by 62 nucleotides MMP_RS07080 and MMP_RS07085 are separated by 118 nucleotides MMP_RS07085 and MMP_RS07090 overlap by 1 nucleotides
MMP_RS07075: MMP_RS07075 - phosphomannomutase/phosphoglucomutase, at 1,353,065 to 1,354,378
_RS07075
MMP_RS07080: MMP_RS07080 - formate--phosphoribosylaminoimidazolecarboxamide ligase, at 1,354,441 to 1,355,526
_RS07080
MMP_RS07085: MMP_RS07085 - ribonuclease HII, at 1,355,645 to 1,356,373
_RS07085
MMP_RS07090: MMP_RS07090 - tRNA (adenine-N1)-methyltransferase, at 1,356,373 to 1,357,251
_RS07090
Position (kb)
1354
1355
1356 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1353.580 kb on - strand, within MMP_RS07075 at 1353.603 kb on - strand, within MMP_RS07075 at 1353.707 kb on + strand, within MMP_RS07075 at 1353.817 kb on + strand, within MMP_RS07075 at 1353.886 kb on - strand, within MMP_RS07075 at 1353.951 kb on + strand, within MMP_RS07075 at 1353.951 kb on + strand, within MMP_RS07075 at 1354.150 kb on + strand, within MMP_RS07075 at 1354.273 kb on - strand at 1354.273 kb on - strand at 1354.357 kb on + strand at 1354.366 kb on + strand at 1354.369 kb on - strand at 1354.438 kb on + strand at 1354.447 kb on - strand at 1354.449 kb on - strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.604 kb on + strand at 1354.669 kb on - strand at 1354.693 kb on - strand at 1354.761 kb on - strand at 1354.769 kb on - strand at 1354.769 kb on - strand at 1354.770 kb on + strand at 1354.780 kb on - strand at 1354.780 kb on - strand at 1354.889 kb on - strand at 1355.544 kb on + strand at 1355.555 kb on + strand at 1355.590 kb on - strand at 1355.591 kb on + strand at 1355.591 kb on + strand at 1355.635 kb on + strand at 1355.635 kb on + strand at 1355.642 kb on + strand at 1355.642 kb on + strand at 1355.642 kb on + strand at 1355.642 kb on + strand at 1355.644 kb on + strand at 1355.862 kb on + strand, within MMP_RS07085 at 1355.862 kb on + strand, within MMP_RS07085 at 1355.862 kb on + strand, within MMP_RS07085 at 1355.863 kb on - strand, within MMP_RS07085 at 1355.866 kb on + strand, within MMP_RS07085 at 1355.868 kb on - strand, within MMP_RS07085 at 1355.869 kb on + strand, within MMP_RS07085 at 1355.940 kb on - strand, within MMP_RS07085 at 1355.993 kb on + strand, within MMP_RS07085 at 1356.013 kb on + strand, within MMP_RS07085 at 1356.023 kb on + strand, within MMP_RS07085 at 1356.035 kb on + strand, within MMP_RS07085 at 1356.083 kb on + strand, within MMP_RS07085 at 1356.182 kb on + strand, within MMP_RS07085 at 1356.379 kb on + strand at 1356.393 kb on - strand at 1356.395 kb on - strand at 1356.395 kb on - strand at 1356.396 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 1,353,580 - MMP_RS07075 0.39 -2.3 1,353,603 - MMP_RS07075 0.41 -2.9 1,353,707 + MMP_RS07075 0.49 -5.5 1,353,817 + MMP_RS07075 0.57 -4.1 1,353,886 - MMP_RS07075 0.62 -4.5 1,353,951 + MMP_RS07075 0.67 -1.8 1,353,951 + MMP_RS07075 0.67 -5.2 1,354,150 + MMP_RS07075 0.83 -3.6 1,354,273 - -1.9 1,354,273 - -2.2 1,354,357 + -0.1 1,354,366 + -0.8 1,354,369 - +0.1 1,354,438 + +0.6 1,354,447 - -1.3 1,354,449 - -2.1 1,354,450 + -0.8 1,354,450 + -2.1 1,354,604 + -1.8 1,354,669 - -2.9 1,354,693 - -4.0 1,354,761 - -0.3 1,354,769 - -1.9 1,354,769 - -0.9 1,354,770 + -1.9 1,354,780 - -2.5 1,354,780 - -1.6 1,354,889 - -1.7 1,355,544 + -1.0 1,355,555 + -1.5 1,355,590 - -0.5 1,355,591 + -2.1 1,355,591 + -3.7 1,355,635 + +0.9 1,355,635 + +0.9 1,355,642 + +0.4 1,355,642 + -0.4 1,355,642 + +0.3 1,355,642 + +1.0 1,355,644 + +0.5 1,355,862 + MMP_RS07085 0.30 +0.6 1,355,862 + MMP_RS07085 0.30 +1.0 1,355,862 + MMP_RS07085 0.30 +1.0 1,355,863 - MMP_RS07085 0.30 +0.2 1,355,866 + MMP_RS07085 0.30 +0.8 1,355,868 - MMP_RS07085 0.31 +0.7 1,355,869 + MMP_RS07085 0.31 +0.9 1,355,940 - MMP_RS07085 0.40 +0.2 1,355,993 + MMP_RS07085 0.48 -0.4 1,356,013 + MMP_RS07085 0.50 -0.2 1,356,023 + MMP_RS07085 0.52 -0.9 1,356,035 + MMP_RS07085 0.53 -0.3 1,356,083 + MMP_RS07085 0.60 +0.4 1,356,182 + MMP_RS07085 0.74 +0.1 1,356,379 + -1.7 1,356,393 - -3.1 1,356,395 - -0.8 1,356,395 - -0.9 1,356,396 + -3.5
Or see this region's nucleotide sequence