Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04700 and MMP_RS04705 are separated by 14 nucleotides MMP_RS04705 and MMP_RS04710 are separated by 29 nucleotides MMP_RS04710 and MMP_RS04715 are separated by 185 nucleotides MMP_RS04715 and MMP_RS04720 are separated by 14 nucleotides
MMP_RS04700: MMP_RS04700 - hypothetical protein, at 899,384 to 899,962
_RS04700
MMP_RS04705: MMP_RS04705 - ribonuclease Z, at 899,977 to 900,903
_RS04705
MMP_RS04710: MMP_RS04710 - hypothetical protein, at 900,933 to 901,325
_RS04710
MMP_RS04715: MMP_RS04715 - CBS domain-containing protein, at 901,511 to 901,891
_RS04715
MMP_RS04720: MMP_RS04720 - DedA family protein, at 901,906 to 902,388
_RS04720
Position (kb) 900
901
902 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 900.074 kb on + strand at 900.265 kb on - strand at 900.298 kb on - strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.323 kb on + strand at 900.464 kb on - strand at 900.465 kb on + strand at 900.501 kb on - strand at 900.913 kb on - strand at 900.914 kb on + strand at 900.914 kb on + strand at 900.914 kb on + strand at 900.916 kb on + strand at 900.939 kb on - strand at 900.940 kb on + strand at 901.094 kb on - strand, within MMP_RS04710 at 901.169 kb on - strand, within MMP_RS04710 at 901.272 kb on + strand, within MMP_RS04710 at 901.286 kb on - strand at 901.304 kb on - strand at 901.307 kb on - strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.341 kb on - strand at 901.360 kb on - strand at 901.362 kb on - strand at 901.362 kb on - strand at 901.362 kb on - strand at 901.363 kb on + strand at 901.363 kb on + strand at 901.363 kb on + strand at 901.365 kb on + strand at 901.365 kb on + strand at 901.365 kb on + strand at 901.383 kb on - strand at 901.393 kb on - strand at 901.402 kb on + strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.404 kb on + strand at 901.404 kb on + strand at 901.404 kb on + strand at 901.407 kb on - strand at 901.407 kb on - strand at 901.408 kb on + strand at 901.423 kb on + strand at 901.453 kb on - strand at 901.470 kb on - strand at 901.470 kb on - strand at 901.474 kb on - strand at 901.474 kb on - strand at 901.475 kb on + strand at 901.476 kb on - strand at 901.476 kb on - strand at 901.477 kb on + strand at 901.477 kb on + strand at 901.478 kb on - strand at 901.478 kb on - strand at 901.479 kb on + strand at 901.479 kb on + strand at 901.481 kb on - strand at 901.511 kb on + strand at 901.582 kb on - strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.598 kb on + strand, within MMP_RS04715 at 901.620 kb on - strand, within MMP_RS04715 at 901.626 kb on - strand, within MMP_RS04715 at 901.627 kb on + strand, within MMP_RS04715 at 901.636 kb on - strand, within MMP_RS04715 at 901.648 kb on - strand, within MMP_RS04715 at 901.649 kb on + strand, within MMP_RS04715 at 901.650 kb on - strand, within MMP_RS04715 at 901.702 kb on + strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.740 kb on - strand, within MMP_RS04715 at 901.750 kb on + strand, within MMP_RS04715 at 901.931 kb on + strand at 902.011 kb on + strand, within MMP_RS04720 at 902.012 kb on - strand, within MMP_RS04720 at 902.016 kb on - strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 900,074 + -1.3 900,265 - -2.2 900,298 - -2.5 900,299 + -1.6 900,299 + -1.7 900,299 + -3.7 900,299 + -2.2 900,299 + -3.3 900,299 + -3.4 900,323 + -2.6 900,464 - -5.1 900,465 + -1.4 900,501 - -1.7 900,913 - -0.2 900,914 + -3.0 900,914 + -3.1 900,914 + -2.2 900,916 + -2.1 900,939 - -1.2 900,940 + -2.6 901,094 - MMP_RS04710 0.41 -3.7 901,169 - MMP_RS04710 0.60 -1.0 901,272 + MMP_RS04710 0.86 -1.5 901,286 - -2.2 901,304 - -0.7 901,307 - -2.2 901,308 + -2.6 901,308 + -2.3 901,308 + -2.9 901,308 + -2.4 901,341 - -3.6 901,360 - -0.6 901,362 - -2.1 901,362 - -1.1 901,362 - -0.4 901,363 + -1.2 901,363 + -1.6 901,363 + -1.7 901,365 + -1.1 901,365 + -0.6 901,365 + -1.0 901,383 - -2.7 901,393 - -2.6 901,402 + -2.9 901,403 - -3.1 901,403 - -2.7 901,403 - -1.8 901,404 + -2.0 901,404 + -2.2 901,404 + -2.4 901,407 - -3.7 901,407 - +0.0 901,408 + -1.5 901,423 + -2.1 901,453 - -3.6 901,470 - -1.5 901,470 - -1.2 901,474 - -2.2 901,474 - -0.2 901,475 + -2.4 901,476 - -2.0 901,476 - -1.0 901,477 + -2.6 901,477 + -4.0 901,478 - -0.5 901,478 - -2.9 901,479 + -1.2 901,479 + -1.3 901,481 - -1.0 901,511 + +0.4 901,582 - MMP_RS04715 0.19 -0.1 901,583 + MMP_RS04715 0.19 -0.9 901,583 + MMP_RS04715 0.19 -0.7 901,598 + MMP_RS04715 0.23 +0.5 901,620 - MMP_RS04715 0.29 -0.5 901,626 - MMP_RS04715 0.30 -0.4 901,627 + MMP_RS04715 0.30 -0.6 901,636 - MMP_RS04715 0.33 +0.8 901,648 - MMP_RS04715 0.36 -0.0 901,649 + MMP_RS04715 0.36 +0.2 901,650 - MMP_RS04715 0.36 +0.1 901,702 + MMP_RS04715 0.50 +0.1 901,710 - MMP_RS04715 0.52 +0.0 901,710 - MMP_RS04715 0.52 -0.9 901,729 - MMP_RS04715 0.57 -0.1 901,729 - MMP_RS04715 0.57 +0.3 901,740 - MMP_RS04715 0.60 +0.7 901,750 + MMP_RS04715 0.63 +0.5 901,931 + -2.5 902,011 + MMP_RS04720 0.22 -1.7 902,012 - MMP_RS04720 0.22 -3.9 902,016 - MMP_RS04720 0.23 -2.0 902,017 + MMP_RS04720 0.23 -3.7 902,017 + MMP_RS04720 0.23 -4.9
Or see this region's nucleotide sequence